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2 protocols using amphetamine d5

1

Comprehensive Reference Standards for Drug Analysis

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The reference standards were obtained from BD Biosciences (Franklin Lakes, NJ, United States), Cayman Chemicals (Ann Arbor, MI, United States), Cerilliant (Round Rock, TX, United States), Chiron AS (Trondheim, Norway), LGC Standards (Wesel, Germany), Lipomed AG (Arlesheim, Switzerland), Sigma-Aldrich (Schnelldorf, Germany), Toronto Research Chemicals (North York, ON, Canada), USP (Rockville, MD, United States), Wyeth Pharmaceuticals LLC (Collegeville, PA, United States), and standards donated by the Slovenian National Forensic Laboratory. The internal standards (ISs) amphetamine-d5, diazepam-d5, methadone-d3, mianserin-d3, and morphine-d6 were purchased from Cerilliant (Round Rock, TX, United States). Acetonitrile, ammonium acetate, ammonium formate, formic acid, and methanol were of LC-MS grade and obtained from Fisher Scientific (Loughborough, United Kingdom).
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2

Optimized LC-MS Workflow for DOA

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Optima LC/MS grade methanol (MeOH), LC/MS grade 0.1% formic acid in water, LC/MS grade formic acid ampules, optima grade dichloromethane (DCM), optima LC/MS grade isopropanol (IPA), hydrochloric acid HCl, tert-butyl methyl ether, (MTBE), and hexane were purchased from Fisher Scientific. Ammonium hydroxide (NH4OH was purchased from Sigma. DOA analytical standards: (+/-) amphetamine, (+/-) methamphetamine, cocaine, benzoylecgonine, morphine, phencyclidine, (-) delta9-THC, (+/-) amphetamine -D5, (+/-) methamphetamine -D5, cocaine -D3, benzoylecgonine -D3, morphine -D3, phencyclidine -D5, and (-) delta9-THC -D3 were obtained as 1mg/mL solutions from Cerilliant (Round Rock, TX, USA). Interfering substances: Diphenhydramine HCl, Alprazolam, Dihydrocodeine, S-Nicotine, Caffeine, Cortisol, -Cotinine, Dextromethorphan, Ibuprofen, Naproxen, Prednisone, R-Pseudoephedrine, Amobarbital, Propranolol, Nicotinamide, Carbamazepine, Clobazam, Clonazepam, Valproic Acid, Verapamil, and Sertraline were purchased from Cerilliant (Round Rock, TX, USA). Stabilization buffer was purchased from Immunalysis (Pomona, CA). The Raptor Bipheyl LC column; 2.7um, 100mm x 2.1mm was purchased from Restek (Bellefonte, PA). Isolute SLE+ columns were purchased from Biotage (Charlotte, NC). The PRESSURE+ 48 positive pressure manifold was obtained from Biotage (Charlotte, NC).
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