Sodium hydroxide (naoh)

Coconut palm petiole woody powder,provided byHainan Kunlun New Material Science & Technology Co.,Ltd.as source of cellulose in this study was cleaned with water, air dried, broken to the size of 2–3 mm × 6–7 mm with a L-905 shredder, grinded into powders with a FZ102 miniature plants grinder (TAISITE instrument Co., Tianjin), and sieved under 100 mesh (Zhang Xing Sand Screen Factory, Zhejiang province).Chemical composition of the coconut palm petiole powder was preliminarily investigated as showing in Table 1 [18 ]. Ethanol,toluene, hydrochloric acid, acidified sodium chlorite and sodium hydroxide pellets were purchased from Nanjing Chemical Reagent Co., Ltd. and all reagents were analytical grade.

Curcumin, ABTS (2,2′-azinobis-3-ethylbenzothiazolin-6-sulfonic acid), and DPPH (1,1′-diphenyl-2-picryhydrazyl) were obtained from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). β-CD (chemical purity) was purchased from Shangdong Xinda Biotechnology Co., Ltd. (Zibo, China). Epichlorohydrin (EP) and ethylene glycol were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). RPMI 1640 medium were purchased from Hyclone Company (South Logan, UT, USA). Fetal bovine serum (FBS) and the antibiotic mixture (penicillin-streptomycin) were purchased from Gibco Company (Grand Island, NY, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Company (Kumamoto, Japan). Caspase 3 assay kit (ab39401), Caspase 8 assay kit (ab39700), and Caspase 9 assay kit (ab65608) were purchased from Abcam Company (Cambridge, UK). Annexin V/PI apoptosis kit were purchased from MultiScience Company (Hangzhou, China). Sodium hydroxide and other reagents were of analytical grade and were purchased from Nanjing Chemical Reagent Co. Ltd. (Nanjing, China). Double distilled and sterilized water was used to prepare all solutions.

Sodium hydroxide (≥96.0%) and anhydrous ethanol (≥99.7%) were purchased from the Nanjing Chemical Reagent Co. Ltd., Nanjing, China. Sulfuric acid (98.0%) and lead nitrate (≥99.0%) were purchased from the Damao Chemical Reagent Co. Ltd., Tianjin, China. Hydrochloric acid (1%) was purchased from the Guangdong Minggu Chemical Technology Co. Ltd., Shanghai, China. Lysozyme and methylene blue (MB) were purchased from the Shanghai Boao Biotechnology Co. Ltd., Shanghai, China. Spent PS foams were collected, rinsed with ethanol and dried at 60 °C in a vacuum overnight.