The 4 T1 murine breast cancer cell line and LM8 murine osteosarcoma cell line were obtained from ATCC (Manassas, VA). Both cell lines were maintained in Dulbecco modified Eagle medium containing 10% fetal bovine serum (Life Technologies, Carlsbad, CA) at 37 °C and in an atmosphere containing 5% CO2.
4t1 murine breast cancer cell line
Manufactured by American Type Culture Collection
Sourced in United States
The 4T1 murine breast cancer cell line is a well-established model for studying triple-negative breast cancer. It is derived from a spontaneously arising mammary tumor in a BALB/c mouse. The 4T1 cell line is highly aggressive and metastatic, making it a valuable tool for cancer research.
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Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (7 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640, by Merck Group (2 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (2 mentions)
In situ cell death detection kit, by Roche (2 mentions)
Ct26 colon carcinoma cell line, by American Type Culture Collection (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin solution, by Corning (1 mentions)
Human mda mb 231 breast adenocarcinoma cell line, by American Type Culture Collection (1 mentions)
Ix81 epifluorescence microscope, by Olympus (1 mentions)
Penicillin streptomycin antibiotic, by Thermo Fisher Scientific (1 mentions)
Antibiotic antimycotic solution, by Welgene (1 mentions)
Dulbecco modified eagle medium (dmem), by Welgene (1 mentions)
1 2 distearoyl sn glycero 3 phosphatidylcholine dspc, by Avanti Polar Lipids (1 mentions)
Annexin 5 fitc apoptosis kit, by Abcam (1 mentions)
Ir 780 iodide, by Merck Group (1 mentions)
Mitosox red mitochondrial superoxide indicator, by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Sodium pyruvate, by Fujifilm (1 mentions)
17 protocols using 4t1 murine breast cancer cell line
1
Culturing Murine Breast and Osteosarcoma Cells
2
Murine Cancer Cell Line Propagation
3
Photothermal Therapy of Tumor Cells
4
Murine Cancer Cell Propagation Protocol
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The 4T1 murine breast-cancer cell line and CT26 murine colorectal cancer-cell line were originally obtained from American Type Culture Collection (ATCC) and cultured in the recommended medium and condition. FLuc-4T1 cancer-cell line was purchased from Hanyin Biotechnology Co., Ltd.
5
Establishing Murine Breast Cancer Models
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The 4T1 murine breast cancer cell line was obtained from the American Type Culture Collection. Firefly luciferase stably transfected 4T1 (4T1-fLuc) cells were generated using a previously described method 22 (link). Cells were maintained in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) at 37°C in humidified atmosphere containing 5% CO2.
All animal experiments were performed in accordance with the Guidelines of the Peking University Animal Care and Use Committee. To establish the 4T1 subcutaneous tumor model, 1 × 106 4T1 tumor cells were inoculated subcutaneously into the right hind legs of BALB/c mice. For the second tumor challenge studies, mice were injected subcutaneously with 1 × 106 4T1 tumor cells into the left hind legs, and six days later with 2 × 105 tumor cells into the right hind legs. For the lung metastatic tumor model, 5 × 105 4T1-fLuc cells were injected via tail vein into BALB/c mice. For the dual subcutaneous and lung metastatic tumor model, 4 × 105 4T1-fLuc tumor cells were inoculated subcutaneously into the right hind legs and 5 × 104 4T1-fLuc tumor cells were injected into the mice through the tail vein. The growth of subcutaneous tumors was measured using a caliper, and the tumor volume was calculated using the following formula: tumor volume = length × (width2)/2.
All animal experiments were performed in accordance with the Guidelines of the Peking University Animal Care and Use Committee. To establish the 4T1 subcutaneous tumor model, 1 × 106 4T1 tumor cells were inoculated subcutaneously into the right hind legs of BALB/c mice. For the second tumor challenge studies, mice were injected subcutaneously with 1 × 106 4T1 tumor cells into the left hind legs, and six days later with 2 × 105 tumor cells into the right hind legs. For the lung metastatic tumor model, 5 × 105 4T1-fLuc cells were injected via tail vein into BALB/c mice. For the dual subcutaneous and lung metastatic tumor model, 4 × 105 4T1-fLuc tumor cells were inoculated subcutaneously into the right hind legs and 5 × 104 4T1-fLuc tumor cells were injected into the mice through the tail vein. The growth of subcutaneous tumors was measured using a caliper, and the tumor volume was calculated using the following formula: tumor volume = length × (width2)/2.
6
Cytotoxicity Evaluation of MnO2-BSA and 131I
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The 4T1 murine breast cancer cell line and CT26 murine colon cell line were originally obtained from American Type Culture Collection (ATCC) and cultured under recommended conditions. The cells were cultured in RPMI-1640 medium plus 10% fetal bovine serum and incubated at 37 °C under 5% CO2 in a cell incubator. 4T1 cells and CT26 cells were seeded in 96-well plates with a density of 5000 cells per well overnight to allow cell adherence. MnO2-BSA, free 131I, 131I-MnO2-BSA were co-cultured with 4T1 cells and CT26 cells at different concentrations for 48 h, respectively. Then, their viabilities were evaluated according to the CCK8 assay kit protocol for determining in vitro cytotoxicity.
7
Culturing Breast Cancer Cell Lines
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The authenticated 4T1 murine breast cancer cell line, luciferase-expressing 4T1-Luc cell line and MDA-MB-231 human breast adenocarcinoma cell line were obtained from American Type Culture Collection (Rockville, USA). All cell lines were cultured at 37°C with 5% CO2 in DMEM supplemented with 10% Fetal Bovine Serum (FBS) (Gibco, USA), and 1% Penicillin-Streptomycin Solution (Corning, NY, USA).
8
Cell Uptake of GO-PEG and HPPH Compounds
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The 4T1 murine breast cancer cell line was obtained from American Type Culture Collection (ATCC, Manassas, VA). The cells were cultured in RPMI-1640 medium supplemented with 10% FBS (GIBCO, Grand Island, NY) and 1% penicillin/streptomycin (100 mg/ml) solution at 37 °C and 5% CO2. For cell uptake assay, the cells were plated in Lab Tek II 8-well chamber slides (Nalge Nunc Internationl, Rochester, NY) with a density of 1 × 104 cells/ml and grown to 60-80% confluency. Then the cells were incubated in the dark for 24 h with GO-PEG, GO-PEG-HPPH and free HPPH at the same HPPH concentration of 1 µM. The cells were then washed with 1× phosphate buffered saline (PBS) three times. Images were acquired by an IX81 epifluorescence microscope (Olympus, Hamburg, Germany).
9
Establishing Murine Breast Cancer Models
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The 4T1 murine breast cancer cell line was obtained from the American Type Culture Collection and cultured as previously described 11 (link). Firefly luciferase stably-transfected 4T1 (4T1-fLuc) cells were generated and cultured as previously described 22 (link). Female BALB/c normal mice (5 weeks of age) were purchased from Department of Laboratory Animal Science, Peking University. All animal studies were performed in accordance with the Guidelines of Peking University Animal Care and Use Committee. To establish a subcutaneous 4T1 tumor mouse model, female BALB/c mice were subcutaneously injected with 1 × 106 4T1 cells into the right hind legs. Tumor growth was measured using a caliper, and tumor volume was calculated using the formula: volume = length × width2/2.
For development of a lymph node metastasis mouse model, 2 × 105 4T1-fLuc cells were injected into the right hind footpads of female BALB/c mice 23 (link). The growth of lymph node metastatic tumors was monitored by bioluminescence imaging (BLI) of anesthetized mice using an in vivo IVIS optical imaging system (Xenogen, Alameda, CA) starting from 10 min after D-luciferin administration (150 mg/kg by intraperitoneal injection).
For development of a lymph node metastasis mouse model, 2 × 105 4T1-fLuc cells were injected into the right hind footpads of female BALB/c mice 23 (link). The growth of lymph node metastatic tumors was monitored by bioluminescence imaging (BLI) of anesthetized mice using an in vivo IVIS optical imaging system (Xenogen, Alameda, CA) starting from 10 min after D-luciferin administration (150 mg/kg by intraperitoneal injection).
10
4T1 Murine Breast Cancer Cell Culture
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4T1 murine breast cancer cell line was purchased from American Type Culture Collection (ATCC) (ATCC, Rockville, MD, USA). The cells were maintained in a complete growing media (RPMI-1640) (Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) (GIBCO, USA), and 1% penicillin-streptomycin antibiotic (GIBCO, Gaithersburg, MD, USA). All cells were grown in culture flasks kept at 37 °C and 5% CO2 [22 (link)].
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