Capcell pak mgii column

At necropsy, samples of approximately 100 mg of PXB mouse liver were collected and added to 300 µL of methanol, respectively. After extraction using a ball mill and then centrifugation at 16,000 × g for 5 min, the supernatant was collected and subjected to LC/MS/MS analysis. The analysis was performed on a Shimadzu 20A HPLC system (Shimadzu Corporation, Kyoto, Japan) equipped with a CAPCELL PAK MGII column (50 mm × 2.0 mm, particle size 3 µm, SHISEIDO CO., LTD., Tokyo, Japan). The conditions were as follows: mobile phase, 0.1% formic acid in water (A) and acetonitrile (B); flow rate, 1.0 mL/min; gradient conditions, 30% B at 0 min, 76% B at 4.0 min, 100% B at 4.0-4.5 min, and 30% B at 4.5-5.0 min; injection volume, 5 µL; and column oven temperature, room temperature. An LTQ Orbitrap XL system (Thermo Fisher Scientific Inc., Waltham, MA, USA) was used to perform the MS/MS analysis in positive ESI mode. The selective reaction monitoring transition was m/z 518/473 for epyrifenacil and m/z 490/473 for S-3100-CA. The amounts of the analytes in the samples were calculated with the linear calibration line including the analytical standards.

In all 23 autopsy cases, qualitative and quantitative analysis of blood collected from the external iliac vein using liquid chromatography (LC)-tandem mass spectrometry (MS/MS) determined that death was caused by intoxication due to drug overdose. Every case demonstrated lethal, supra-therapeutic drug levels. LC was performed with a P r o m i n e n c e L C s y s t e m ( S h i m a d z u , K y o t o , J a p a n ) . Chromatographic separation was achieved on a CAPCELL-PAK MG II column (35.0 × 2.0 mm id, 5 lm; Shiseido, Tokyo, Japan). MS/MS detection was performed with a 3200 QTRAP system equipped with an electrospray ionization probe (AB Sciex, Foster City, CA). All analyses were performed by the forensic toxicologist (K.U.) in charge of laboratory sample testing at our institution.