Plasma FHR-5 was detected by enzyme-linked immunosorbent assay as previous described.20 (link),21 (link) Briefly, rabbit antihuman FHR-5 antibody (Abnova Corp., Taipei, Taiwan) was coated onto half the wells of a microtiter plate (Thermo Fisher Scientific, Waltham, MA) as the capture antibody. After blocking with 1% BSA, the standard control (recombinant human FHR-5 protein; R&D Systems, Minneapolis, MN), plasma samples and quality controls were added and incubated for 1 hour at room temperature. Binding of FHR-5 was detected using mouse anti–FHR-5 antibodies (Abnova Corp., Taipei, Taiwan). After the addition of horseradish peroxidase-conjugated rabbit antimouse immunoglobulin G (Dako), the reaction was developed using the peroxidase chromogenic substrate 3,3,5,5-tetramethylbenzididine liquid substrate system (Sigma Chemical Co., St. Louis, MO) and stopped with 1 mol/l H2SO4 (sulfuric acid) before the absorbance was measured at 450 and 570 nm.
Rabbit antihuman fhr 5 antibody
Manufactured by Abnova
Sourced in Taiwan, Province of China
Rabbit antihuman FHR-5 antibody is a laboratory reagent used for the detection and identification of the FHR-5 protein in biological samples. It is a polyclonal antibody produced in rabbits and specifically targets the human FHR-5 protein.
Automatically generated - may contain errors
Lab products found in correlation
Recombinant human fhr 5 protein, by R&D Systems (2 mentions)
Microtiter plate, by Thermo Fisher Scientific (1 mentions)
H2so4, by Merck Group (1 mentions)
Horseradish peroxidase conjugated rabbit anti mouse igg, by Agilent Technologies (1 mentions)
Tetramethylbenzidine liquid substrate system, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using rabbit antihuman fhr 5 antibody
1
Quantification of Plasma FHR-5 Levels
2
Quantifying Circulating FHR-5 Levels
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Circulating FHR-5 was detected by enzyme-linked immunosorbent assay as previously described. 17 Briefly, rabbit anti-human FHR-5 antibody (Abnova Corporation, Taipei, Taiwan) was coated onto half the wells of a microtitre plate (Nunc Immunoplate, Roskilde, Denmark). After blocking with 1% bovine serum albumin (Sigma Chemical Company, St. Louis, MO), the plasma samples were added and incubated for 1 hour at room temperature. Binding of FHR-5 was examined using mouse anti-human FHR-5 antibodies (Abnova Corporation). Following the addition of horseradish peroxidaseconjugated rabbit anti-mouse IgG (DakoCytomation, Glostrup, Denmark), the reaction was developed using the peroxidase chromogenic substrate 3,3 0 ,5,5 0 -Tetramethylbenzidine Liquid Substrate System (Sigma Chemical Company) and stopped with 1 mol/l sulfuric acid before the absorbance was measured at 450 and 570 nm. Serial dilutions of recombinant human FHR-5 protein (R&D Systems, Minneapolis, MN) were used to establish the standard curve, which was then used to calculate circulating FHR-5 levels.
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