The antibodies used in Western blot were pThr58-Myc (Santa Cruz
Biotechnology, Dallas, Texas, sc-135647),
pS62-Myc (Abcam), ERCC3 (A301-337A,
Bethyl Laboratories, Montgomery, TX); antibodies to MYC (#5605),
Rpb1-CTD (#2629),
pSer2-CTD of Rpb1 (#8798),
pSer2-CTD of Rpb1(#8807),
β-actin (#4967) and
α-tubulin(#2125) were purchased from Cell Signaling Technology, Danvers, MA.
Triptolide was purchased from Cayman Chemical (Ann Arbor, MI) and dissolved in
DMSO as 1 mM stock. Chemotherapy drugs were purchased from the Fox Chase Cancer
Center pharmacy.
Beglyarova N., Banina E., Zhou Y., Mukhamadeeva R., Andrianov G., Bobrov E., Lysenko E., Skobeleva N., Gabitova L., Restifo D., Pressman M., Serebriiskii I.G., Hoffman J.P., Paz K., Behrens D., Khazak V., Jablonski S.A., Golemis E.A., Weiner L.M, & Astsaturov I. (2016). Screening of conditionally reprogrammed patient-derived carcinoma cells identifies ERCC3-MYC interactions as a target in pancreatic cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 22(24), 6153-6163.