Collagen (Kollagenreagent Horm; Nycomed), convulxin (Enzo Lifesciences) α-thrombin (Roche Diagnostics), adenosine diphosphate (ADP), sodium heparin, human fibrinogen, apyrase type III, prostacyclin (PGI2), Igepal CA-630 (all from Sigma-Aldrich), U46619 (Alexis Biochemicals) and ECL solution (PerkinElmer) were purchased, collagen-related peptide (CRP) was generated as described [21] (link). Rhodocytin was a generous gift from Prof. Dr. J. Eble (Münster University Hospital, Germany). The anesthetic drugs medetomidine (Pfizer), midazolam (Roche Pharma AG), and fentanyl (Janssen-Cilag GmbH) and the antagonists atipamezol (Pfizer), flumazenil, and naloxon (both from Delta Select GmbH) were used according to the regulation of the local authorities. The antibody against the activated form of integrin αIIbβ3 (JON/A-PE) was from Emfret Analytics. Anti-murine CD84 monoclonal antibody JER1 [19] (link) and other antibodies were generated and modified in our laboratories as described [22] (link).
α thrombin
Manufactured by Roche
Sourced in Germany
α-thrombin is a serine protease enzyme that plays a key role in the blood coagulation cascade. It converts fibrinogen into fibrin, an essential step in the formation of blood clots.
Automatically generated - may contain errors
Lab products found in correlation
Kollagenreagent horm, by Takeda Pharmaceuticals (2 mentions)
Apyrase type 3, by Merck Group (2 mentions)
Human fibrinogen, by Merck Group (1 mentions)
Atipamezol, by Pfizer (1 mentions)
Prostacyclin, by Merck Group (1 mentions)
Heparin sodium, by Merck Group (1 mentions)
Jon a pe, by Emfret Analytics (1 mentions)
Ecl solution, by PerkinElmer (1 mentions)
Igepal ca 630, by Merck Group (1 mentions)
Convulxin, by Enzo Life Sciences (1 mentions)
U46619, by Alexis Biochemicals (1 mentions)
Adenosine diphosphate (adp), by Merck Group (1 mentions)
Medetomidine, by Pfizer (1 mentions)
Electrochemiluminescence solution, by PerkinElmer (1 mentions)
U46619, by Enzo Life Sciences (1 mentions)
G lisa, by Cytoskeleton (1 mentions)
Protease inhibitor cocktail, by Merck Group (1 mentions)
Heparin, by Ratiopharm (1 mentions)
Dioc6 3, by Enzo Life Sciences (1 mentions)
2 protocols using α thrombin
1
Platelet activation assays using agonists
2
Platelet Activation Pathway Analysis
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EHT1864, NSC23766 (Tocris Bioscience, Bristol, UK), collagen (Kollagenreagent Horm; Nycomed, Munich, Germany), ADP, human fibrinogen, apyrase type III, protease inhibitor cocktail (Sigma-Aldrich, Steinheim, Germany), α-thrombin (Roche Diagnostics, Mannheim, Germany), high molecular weight heparin (Ratiopharm, Ulm, Germany), U46619, DiOC 6 (3) (Enzo Life Sciences, L rrach, Germany), electrochemiluminescence solution (Perkin-Elmer, Waltham, MA, USA), G-LISA (Cytoskeleton, Denver, CO, USA), integrilin (eptifibatide; Millennium Pharmaceuticals, Cambridge, MA, USA) and botrocetin (Pentapharm Ltd., Basel, Switzerland) were purchased. Collagen-related peptide (CRP) was generated as described previously 19
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