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Alexa fluor anti human cd83 clone hb15e

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Alexa Fluor® anti human CD83 (clone HB15e) is a fluorescently labeled monoclonal antibody that specifically binds to the CD83 antigen expressed on human cells. This product is suitable for flow cytometry applications.

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Lab products found in correlation

Pe anti mouse cd86, by BD (2 mentions) Pe anti human cd80, by BioLegend (2 mentions) Pe cy7 anti human fcεriα mab cra1 clone aer 37, by Thermo Fisher Scientific (2 mentions) Pe anti mouse ige rme 1, by BioLegend (2 mentions) Alexa fluor 647 anti mouse cd8α, by BioLegend (2 mentions) Alexa fluor 647 anti mouse cd4, by BioLegend (2 mentions) Anti cd117 apc, by BioLegend (2 mentions) Pe anti mouse fcεriα mab mar1, by BioLegend (2 mentions) Pe anti mouse ifn γ clone xmg1, by BioLegend (2 mentions) Apc anti mouse il 4 clone 11b11, by Thermo Fisher Scientific (2 mentions) Apc anti human cd19 clone hib19, by BioLegend (2 mentions) Pe cy7 anti human ige clone mhe 18, by BioLegend (2 mentions) Pe anti human cd203, by BioLegend (2 mentions) Apc anti human cd1a, by BD (2 mentions) Mouse fc block, by BD (2 mentions) Facscanto 2, by BD (2 mentions) Cytofix cytoperm, by BD (2 mentions) Anti cd11c apc, by BioLegend (2 mentions)

2 protocols using alexa fluor anti human cd83 clone hb15e

1

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions were pretreated with mouse Fc-Block™ (BD Biosciences/ Pharmingen) and incubated with fluorophore-labeled antibodies or appropriate isotype controls. Acquisitions were performed using a BD FACSCanto™ II (BD Bioscience). For intracellular staining of cytokines BD Cytofix/Cytoperm (BD Biosciences) was used. Analysis was done using BD FACSDiva™ and FlowJo (Treestar INC.) software. Following antibodies were used: Phycoerythrin-(PE), allophycocyanin-(APC) and PE/Cy7 anti-human FcεRIα mAb CRA1 (clone AER-37, eBioscience or BioLegend), PE-anti-mouse IgE (RME-1, Biolegend), APC-anti-CD11c, Alexa Fluor® 647 anti-mouse CD8α and Alexa Fluor® 647 anti-mouse CD4 from Biolegend (San Diego, CA), PE-anti-mouse CD86 (BD Pharmingen), APC-anti- CD117 (Biolegend), and PE- anti-mouse FcεRIα mAb (Mar1, Biolegend). Intracellular cytokine stain included PE anti-mouse IFN-γ (clone XMG1.2, Biolegend) and APC anti-mouse IL-4 (clone 11B11, eBioscience). Anti-human CD1c (BDCA-1, clone AD5-8E7, Miltenyi Biotech), APC-anti-human CD19 (clone HIB19, BioLegend), PE/Cy7 anti- human IgE (clone MHE-18, BioLegend), PE anti-human CD203 (Biolegend), Alexa Fluor® anti human CD83 (clone HB15e), PE anti-human CD80 (Biolegend) and APC anti-human CD1a (BD Pharmingen).
Platzer B., Baker K., Vera M.P., Singer K., Panduro M., Lexmond W.S., Turner D., Vargas S.O., Kinet J.P., Maurer D., Baron R.M., Blumberg R.S, & Fiebiger E. (2014). Dendritic cell-bound IgE functions to restrain allergic inflammation at mucosal sites. Mucosal immunology, 8(3), 516-532.
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2

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions were pretreated with mouse Fc-Block™ (BD Biosciences/ Pharmingen) and incubated with fluorophore-labeled antibodies or appropriate isotype controls. Acquisitions were performed using a BD FACSCanto™ II (BD Bioscience). For intracellular staining of cytokines BD Cytofix/Cytoperm (BD Biosciences) was used. Analysis was done using BD FACSDiva™ and FlowJo (Treestar INC.) software. Following antibodies were used: Phycoerythrin-(PE), allophycocyanin-(APC) and PE/Cy7 anti-human FcεRIα mAb CRA1 (clone AER-37, eBioscience or BioLegend), PE-anti-mouse IgE (RME-1, Biolegend), APC-anti-CD11c, Alexa Fluor® 647 anti-mouse CD8α and Alexa Fluor® 647 anti-mouse CD4 from Biolegend (San Diego, CA), PE-anti-mouse CD86 (BD Pharmingen), APC-anti- CD117 (Biolegend), and PE- anti-mouse FcεRIα mAb (Mar1, Biolegend). Intracellular cytokine stain included PE anti-mouse IFN-γ (clone XMG1.2, Biolegend) and APC anti-mouse IL-4 (clone 11B11, eBioscience). Anti-human CD1c (BDCA-1, clone AD5-8E7, Miltenyi Biotech), APC-anti-human CD19 (clone HIB19, BioLegend), PE/Cy7 anti- human IgE (clone MHE-18, BioLegend), PE anti-human CD203 (Biolegend), Alexa Fluor® anti human CD83 (clone HB15e), PE anti-human CD80 (Biolegend) and APC anti-human CD1a (BD Pharmingen).
Platzer B., Baker K., Vera M.P., Singer K., Panduro M., Lexmond W.S., Turner D., Vargas S.O., Kinet J.P., Maurer D., Baron R.M., Blumberg R.S, & Fiebiger E. (2014). Dendritic cell-bound IgE functions to restrain allergic inflammation at mucosal sites. Mucosal immunology, 8(3), 516-532.
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