Cells in the exponential growth phase (106 cells/ml, 10 μl of the culture) were placed on a thin agarose pad (1.5%), and the pad was subsequently placed down on a glass dish, resulting in a monolayer of cells between the agarose pad and glass dish. The culture condition was the same as the single colony assay experiment. Images were acquired at 60× magnification using a fluorescence microscope (TE2000; Nikon) and a cooled CCD camera (DV887; Andor). The gain of the camera was 100, and exposure time was 50 ms. The images were analyzed using ImageJ software (NIH) to measure cell length.
Dv887
Manufactured by Oxford Instruments
The DV887 is a high-performance lab equipment product manufactured by Oxford Instruments. It is designed to provide precise and reliable measurements for scientific and research applications. The core function of the DV887 is to perform advanced analytical tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
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2 protocols using dv887
1
Measuring Cell Length via Microscopy
2
Dynamic Interferometric Imaging Setup
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The excitation signal for the sample piezo-element (Pz26 26302, Ferroperm) and the pulse signal for the LED (ELD-870f-515-2, Roithner Laser) are generated by a double function generator (DG 4162, Rigol). The piezo-excitation signal amplitude is set to 30 mV for frequencies from 1.7 to 2.1 MHz and 50 mV otherwise before amplification by an amplifier (2200L, E&I) with a fixed power gain of 53 dB. This results in a peak amplitude at the piezo of approximately 50 Vpp around the piezo-resonance at 2 MHz. The pulse signal is amplified and added to a bias using a homebuilt amplifier.
The axial translation stage for the reference mirror is based on a piezo-actuator (PXY80D12, Piezosystem Jena), controlled by a homebuilt position sensor and feedback electronics to ensure linear dependence of position on control voltage. The function generator, control voltage, triggering, and readout of the camera (Ixon DV887, Andor) are controlled by a PC using a data acquisition card (USB-6212, National Instruments) and a homebuilt control program (Labview 2011, National Instruments).
The axial translation stage for the reference mirror is based on a piezo-actuator (PXY80D12, Piezosystem Jena), controlled by a homebuilt position sensor and feedback electronics to ensure linear dependence of position on control voltage. The function generator, control voltage, triggering, and readout of the camera (Ixon DV887, Andor) are controlled by a PC using a data acquisition card (USB-6212, National Instruments) and a homebuilt control program (Labview 2011, National Instruments).
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