Lxsarm

Manufactured by R&D Systems

The LXSARM is a laboratory equipment product developed by R&D Systems. It is designed for general laboratory use. The core function of the LXSARM is to provide a reliable and versatile platform for various experimental and analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

Ab119558, by Abcam (2 mentions) Ab100778, by Abcam (2 mentions) Ab213906, by Abcam (2 mentions) Era32rb, by Thermo Fisher Scientific (1 mentions) Tissueruptor, by Qiagen (1 mentions) Ab105136, by Abcam (1 mentions) Adi 900 155, by Enzo Life Sciences (1 mentions) Ls f5638, by LifeSpan BioSciences (1 mentions)

4 protocols using lxsarm

Inflammatory Cytokine Analysis in Atrial Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Atrial tissue was minced and homogenized using a tissue homogenizer (TissueRuptor, QIAGEN) according to the manufacturer protocol. Inflammatory cytokines and chemokines were measured using a multiplex Luminex-based assay (LXSARM, R&D Systems). Each sample was run in duplicate in a 96-well plate. IL-1β, IL-2, IL-18, and TNF-α were measured using a MAGPIX system (C4447b, Luminex). Acquired mean fluorescence data were analyzed and calculated by xPONENT software. IL-6 (ERA32RB, Invitrogen), TGF-β1 (ab119558, Abcam), PDGF-AB (ab213906, Abcam), and MCP-1 (ab100778, Abcam) were quantified using commercially available ELISAs according to the manufacturers’ protocols.

Parent S., Vaka R., Risha Y., Ngo C., Kanda P., Nattel S., Khan S., Courtman D., Stewart D.J, & Davis D.R. (2023). Prevention of atrial fibrillation after open-chest surgery with extracellular vesicle therapy. JCI Insight, 8(15), e163297.

+ Open protocol

+ Expand

Cytokine Profiling in Rat Muscle

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following euthanasia and after serum collection, flexor digitorum muscles from 5 FRC rats and 5 HRHF-CON rats were collected from the distal forearm region of the preferred reach limb. Muscles were homogenized, as previously described (Barbe, Gallagher et al. 2013 (link)), and assayed by customized multiplex bead-based analysis kits (LXSARM, R&D Systems) for IL-1α, IL-6, IL-1β, and TNFα. ELISA assay data (pg of cytokine protein) were normalized to μg total protein, determined using a bicinchoninic acid protein assay kit.

Bove G.M., Harris M.Y., Zhao H, & Barbe M.F. (2015). Manual therapy as an effective treatment for fibrosis in a rat model of upper extremity overuse injury. Journal of the neurological sciences, 361, 168-180.

+ Open protocol

+ Expand

Atrial Inflammation Biomarkers Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Homogenized atrial tissue obtained after the invasive electrophysiological study was analyzed for markers of atrial inflammation using ELISAs and a custom multiplex Luminex-based assay (LXSARM, R&D Systems). Interleukin 6 (IL-6, ERA32RB, Life Sciences), IL-17 (MBS2022678, MyBioSource), platelet-derived growth factor AB (PDGF-AB, ab213906, Abcam), monocyte chemoattractant protein-1 (MCP-1, ab100778, Abcam) and transforming growth factor beta 1 (TGFβ1; ab119558, Abcam) were analyzed using commercially available ELISAs. Four analytes (IL-1β, IL-10, IL-18, tumor necrosis factor alpha (TNFα)) were measured using a MAGPIX system (LXSARM-06) with post-hoc mean fluorescence quantification using xPONENT software. Atrial myeloperoxidase activity was evaluated using a colorimetric assay according to the manufacturer's directions (ab105136, Abcam).

Parent S., Vaka R., St Amant J., Kahn S., Van Remortel S., Bi C., Courtman D., Stewart D.J, & Davis D.R. (2024). Inactivation of the NLRP3 inflammasome mediates exosome-based prevention of atrial fibrillation. Theranostics, 14(2), 608-621.

+ Open protocol

+ Expand

Muscle Collagen and Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animals were anesthetized and tissues for ELISA removed prior to perfusion of rats with fixative. Blood and muscle tissues were collected and prepared for ELISA as described in Supplemental Methods. Serum and muscle lysates (n=5-12/gp) were assayed for Collagen type 1 (LS-F5638, LifeSpan BioSciences, Seattle, WA) and transforming growth factor beta 1 (TGFβ1; ADI-900-155, Enzo, Farmingdale, NY). Muscle lysates (n=6-11/group) were also assayed for IL-1alpha, IL-1beta, CXCL1, CCL2, TNFα (tumor necrosis factor alpha) and VEGF (vascular endothelial growth factor) (RV1MAG-26K, Merk Millipore; LXSARM, R&D). All samples were run in duplicate. Total protein content of muscles was determined using BCA Protein Assays, and muscle data is reported as pg of protein per mg of total protein. Serum data is reported as pg of protein per ml of serum.

Barbe M.F., White A.R., Hilliard B.A., Salvadeo D.M., Amin M., Harris M.Y., Cruz G.E., Hobson L, & Popoff S.N. (2019). Comparing effects of rest with or without a NK1RA on fibrosis and sensorimotor declines induced by a voluntary moderate demand task. Journal of Musculoskeletal & Neuronal Interactions, 19(4), 396-411.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!