Hplc grade 2 propanol

Manufactured by Thermo Fisher Scientific

Sourced in United States

HPLC-grade 2-propanol is a high-purity solvent commonly used in high-performance liquid chromatography (HPLC) applications. It serves as a mobile phase component for the separation and analysis of a wide range of chemical compounds.

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HPLC grade (157 citations) 2-propanol (134 citations)

15 protocols using hplc grade 2 propanol

Analytical Method Development for Ketoconazole

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Ketoconazole (lot # BS1203355108, 98% purity) was purchased from Bosche Scientific (New Brunswick, NJ) and used as received. Adipic acid (lot # 06807BE, 99% purity), succinic acid (lot # 037K0021, 99% purity), fumaric acid (lot # 09426EE, 99+% purity), acetic acid (lot # 074K3658, 99%), sodium acetate anhydrous (lot # 100K0272), dipotassium hydrogen phosphate (lot # 103H0287, ACS reagent), and sodium chloride (lot # 094K0183, ACS reagent) were purchased from Sigma-Aldrich (St. Louis, MO) and used as received.
HPLC grade methanol, HPLC grade 2-propanol, sodium phosphate monobasic (lot # 017316), and hydrochloric acid (lot # 2AJK15038, ACS grade) were purchased from Fisher Scientific (Fair Lawn, NJ). Acetone (ACS reagent 99.5%) and phosphoric acid (lot # B0506524, 85+%) were purchased from Acros Organics (NJ) and used as received. Trifluoroacetic acid (spectrophometric grade, 99%) was purchased from Aldrich Company (Milwaukee, WI). NaOH (pellets) was purchased from J.T. Baker (Philipsburg, NJ). Water used in this study was filtered through a double deionized purification system (Milli Q Plus Water System) from Millipore Co. (Bedford, MA).

Chen Y.M, & Rodríguez-Hornedo N. (2018). Cocrystals Mitigate Negative Effects of High pH on Solubility and Dissolution of a Basic Drug. Crystal growth & design, 18(3), 1358-1366.

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HPLC-ECD Quantification of CoQ Homologs

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CoQ homologue concentrations were determined using an HPLC-ECD system, as reported previously,^{(12 (link))} with minor modifications. Briefly, samples were added to a 9-fold volume of HPLC-grade 2-propanol (Fisher Chemicals, Fairlawn, NJ), vigorously mixed, and centrifuged. Supernatants thus obtained were injected onto the HPLC-ECD system. Mobile phase: 50 mM NaClO₄ in methanol/2-propanol (7/3, v/v); flow rate: 1.0 ml/min; analytical column: KANTO RP-18 (L) GP, 5 μm × 150 mm × 4.6 mm (Kanto Chemical, Tokyo, Japan); post-reduction column: RC-10, 15 mm × 4 mm (IRICA, Kyoto, Japan); detector: ECD (600 mV) NANOSPACE SI-1 (Shiseido, Tokyo, Japan).

Hasegawa M., Yamamoto Y., Fujisawa A, & Kashiba M. (2023). Prosaposin is a novel coenzyme Q10-binding protein. Journal of Clinical Biochemistry and Nutrition, 74(2), 108-112.

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Quantifying CoQ10 and Free Cholesterol in Cells

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Concentrations of CoQ10 and free cholesterol (FC) in cells were determined by HPLC as reported previously.^{(4 (link))} Briefly, cells were seeded in 24-well plates and incubated for various times. The cell media were removed and the cells were washed twice with ice cold PBS. Then, 400 µl of HPLC grade 2-propanol (Fisher Chemicals, Fairlawn, NJ) was added. Samples were collected and centrifuged. Extracts thus obtained were injected into the HPLC-ECD system. Mobile phase: 50 mM NaClO₄ in methanol/2-propanol (7/3, v/v); flow rate: 1.0 ml/min; analytical column: KANTO RP-18 (L) GP, 5 µm × 150 mm × 4.6 mm (Kanto Chemical, Tokyo, Japan); post-reduction column: RC-10, 15 mm × 4 mm (IRICA, Kyoto, Japan). CoQ10 and FC concentrations were determined by an electrochemical detector (600 mV; NANOSPACE SI-1, Shiseido, Tokyo, Japan) and a UV detector (210 nm; SPD-10A, Shimadzu, Kyoto, Japan), respectively.
CoQ10 in sucrose gradient fractions was extracted by using a 5-volume quantity of methanol and a 10-volume quantity of hexane. Triolein was used as an internal standard. Hexane fractions thus obtained were dried under a nitrogen gas stream and redissolved in 2-propanol. The samples were directly injected into the HPLC system described above.

Kashiba M., Oizumi M., Suzuki M., Sawamura Y., Nagashima K., Yoshimura S, & Yamamoto Y. (2014). Prosaposin regulates coenzyme Q10 levels in HepG2 cells, especially those in mitochondria. Journal of Clinical Biochemistry and Nutrition, 55(2), 85-89.

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Quantifying CoQ10 and Free Cholesterol

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Concentrations of CoQ10 and free cholesterol (FC) in cells were determined by HPLC, as reported previously.^{(3 (link))} Briefly, cells were seeded in 24-well plates at a density of 30,000/cm² and incubated for various times. The cell media were removed and the cells were washed with ice-cold PBS twice. Then, 400 µl of HPLC grade 2-propanol (Fisher Chemicals, Fairlawn, NJ) was added. Samples were collected and centrifuged. Extracts thus obtained were injected into the HPLC-ECD system. Mobile phase: 50 mM NaClO₄ in methanol/2-propanol (7/3, v/v); flow rate: 1.0 ml/min; analytical column: KANTO RP-18 (L) GP, 5 µm × 150 mm × 4.6 mm (Kanto Chemical, Tokyo, Japan); post-reduction column: RC-10, 15 mm × 4 mm (IRICA, Kyoto, Japan). CoQ10 and FC concentrations were determined by an electrochemical detector (600 mV; NANOSPACE SI-1, Shiseido, Tokyo, Japan) and a UV detector (210 nm; SPD-10A, Shimadzu, Kyoto, Japan), respectively.

Kashiba M., Terashima M., Sagawa T., Yoshimura S, & Yamamoto Y. (2016). Prosaposin knockdown in Caco-2 cells decreases cellular levels of coenzyme Q10 and ATP, and results in the loss of tight junction barriers. Journal of Clinical Biochemistry and Nutrition, 60(2), 81-85.

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Parthenolide-Induced Thyroid Cancer Cell Inhibition

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Parthenolide was purchased from Absin (Shanghai, China), and human PTC cell line BCPAP was purchased from Shanghai Institutes for Biological Sciences, China. RPMI 1640 medium was purchased from Corning, USA. Fetal bovine serum was purchased from Shuangru Biology ScienceandTechnology Co.Ltd. HPLC-grade formic acid and HPLC-grade ammonium formates were purchased from Sigma. MS-grade acetonitrile, MS-grade methanol, and HPLC-grade 2-propanol were purchased from Thermo Fisher.

Huang L.T., Li T.J., Li M.L., Luo H.Y., Wang Y.B, & Wang J.H. (2023). Untargeted lipidomic analysis and network pharmacology for parthenolide treated papillary thyroid carcinoma cells. BMC Complementary Medicine and Therapies, 23, 130.

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Highly Sensitive Mass Spectrometry

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MS-grade methanol (047192), MS-grade acetonitrile (51101), HPLC-grade 2-propanol (022906) were purchased from ThermoFisher (USA). HPLC-grade formic acid (5438040250) and HPLC-grade ammonium formate (714690-4X4L) were purchased from Sigma (Germany).

Chen M., Zhang C., Li H., Zheng S., Li Y., Yuan M., Chen Y., Wu J, & Sun Q. (2024). PLA2G4A and ACHE modulate lipid profiles via glycerophospholipid metabolism in platinum-resistant gastric cancer. Journal of Translational Medicine, 22, 249.

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Metabolite Extraction Protocol Using HPLC

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MS-grade acetonitrile, MS-grade methanol, and HPLC-grade 2-propanol were purchased from Thermo Fisher. HPLC-grade ammonium, HPLC-grade formate Isopropanol, and HPLC-grade formic acid were purchased from Sigma. Additional reagents information can be found in supplementary materials (Table S1).

Cai Y., Zhang S., Chen L, & Fu Y. (2023). Integrated multi-omics and machine learning approach reveals lipid metabolic biomarkers and signaling in age-related meibomian gland dysfunction. Computational and Structural Biotechnology Journal, 21, 4215-4227.

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Comprehensive Herbal Combination Characterization

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MS-grade methanol, MS-grade acetonitrile, HPLC-grade 2-propanol were purchased from Thermo Fisher. HPLC-grade formic acid and HPLC-grade ammonium formate were purchased from Sigma. CSS is composed of seven herbs, including Citrus reticulata Blanco, Radix Bupleuri, Ligusticum chuanxiong Hort, CyperusrotundusL., Citrus aurantium L., Paeonia lactiflora Pall. and Glycyrrhiza uralensis Fisch., and the ratio is 4:4:3:3:3:3:1. The herbs were purchased from the pharmacy of Jiangsu Provincial Hospital of Traditional Chinese Medicine, Nanjing University of Chinese Medicine. All herbs were authenticated by the herbal medicine botanist Professor Yunan, Zhao, Nanjing University of Chinese Medicine.

Li W., Zhou R., Zheng J., Sun B., Jin X., Hong M, & Chen R. (2022). Chaihu-Shugan-San ameliorates tumor growth in prostate cancer promoted by depression via modulating sphingolipid and glycerinphospholipid metabolism. Frontiers in Pharmacology, 13, 1011450.

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Metabolite Quantification Using LC-MS

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Sigma Aldrich provided Ammonium acetate (NH₄AC), Merck provided Acetonitrile, and Fisher provided ammonium hydroxide (NH₄OH) and methanol. Thermo Fisher provided MS-grade methanol, MS-grade Acetonitrile, and HPLC-grade 2-propanol. Sigma provided HPLC-grade formic acid and HPLC-grade ammonium formate. All chemicals used in this study were of high purity and quality, ensuring the reliability and reproducibility of the results.

Qiao C., He M., Wang S., Jiang X., Wang F., Li X., Tan S., Chao Z., Xin W., Gao S., Yuan J., Li Q., Xu Z., Zheng X., Zhao J, & Liu G. (2023). Multi-omics analysis reveals substantial linkages between the oral-gut microbiomes and inflamm-aging molecules in elderly pigs. Frontiers in Microbiology, 14, 1250891.

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Tanshinone Compound Quantification Method

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MS-grade methanol, MS-grade acetonitrile and high-performance liquid chromatography (HPLC)-grade 2-propanol were purchased from Thermo Fisher. HPLC-grade formic acid and HPLC-grade ammonium formate were purchased from Sigma. HPLC-grade acetonitrile was purchased from Merck KGaA (Darmstadt, Germany). Analytical-grade pure phosphoric acid was purchased from Guangzhou Chemical Reagent Factory. Reference standards of Tanshinone I, Crytotanshinone, Tanshinone IIA (purity >98%) were purchased from the National Institutes for Food and Drug Control (Beijing, China). Diydrotanshinone I (purity >98%) was purchased from Guangzhou Qiyun Biotechnology Co., Ltd. (Guangdong, China).

Chen T., Zhu Z., Du Q., Wang Z., Wu W., Xue Y., Wang Y., Wu Y., Zeng Q., Jiang C., Shen C., Liu L., Zhu H, & Liu Q. (2021). A Skin Lipidomics Study Reveals the Therapeutic Effects of Tanshinones in a Rat Model of Acne. Frontiers in Pharmacology, 12, 675659.

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