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Pspc1 antibody

Manufactured by Santa Cruz Biotechnology
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The PSPC1 antibody is a laboratory reagent used for the detection and study of the PSPC1 protein in various biological samples. PSPC1 is a protein involved in the regulation of gene expression and cellular processes. The antibody can be utilized in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to identify and analyze the PSPC1 protein in research applications.

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Lab products found in correlation

Dmi6000 inverted microscope, by Leica (2 mentions)

Close spelling variants of this product

PSPC1

2 protocols using pspc1 antibody

1

Immunostaining of MEFs and ESCs

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Mouse embryonic fibroblasts (MEFs) and ESCs were grown on 24-well plates coated with 0.1% gelatin (w/v). After fixation with 4% paraformaldehyde (w/v) for 15 min, cells were permeabilized with 0.25% Triton X-100 (v/v) in PBS for 5 min and incubated with 10% BSA for 30 min at 37°C. For immunostaining, cells were incubated overnight at 4°C with PSPC1 antibody (Santa Cruz, sc-84577) in PBS with 3% BSA (w/v). The following day cells were incubated with fluorophore-labeled secondary antibodies for 1 h at RT. Cells were imaged with a Leica DMI 6000 inverted microscope.
Huang X., Bashkenova N., Hong Y., Lyu C., Guallar D., Hu Z., Malik V., Li D., Wang H., Shen X., Zhou H, & Wang J. (2022). A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Cell reports, 39(10), 110928.
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2

Immunostaining of MEFs and ESCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse embryonic fibroblasts (MEFs) and ESCs were grown on 24-well plates coated with 0.1% gelatin (w/v). After fixation with 4% paraformaldehyde (w/v) for 15 min, cells were permeabilized with 0.25% Triton X-100 (v/v) in PBS for 5 min and incubated with 10% BSA for 30 min at 37°C. For immunostaining, cells were incubated overnight at 4°C with PSPC1 antibody (Santa Cruz, sc-84577) in PBS with 3% BSA (w/v). The following day cells were incubated with fluorophore-labeled secondary antibodies for 1 h at RT. Cells were imaged with a Leica DMI 6000 inverted microscope.
Huang X., Bashkenova N., Hong Y., Lyu C., Guallar D., Hu Z., Malik V., Li D., Wang H., Shen X., Zhou H, & Wang J. (2022). A TET1-PSPC1-Neat1 molecular axis modulates PRC2 functions in controlling stem cell bivalency. Cell reports, 39(10), 110928.
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