The instruments used in this study were as follows: F-4500 spectrofluorophotometer (Hitachi, Japan); UV-1700 spectrophotometer (Shimadzu, Japan); H-600 transmission electron microscope (Hitachi, Japan); DB-525 Zeta Potential Analyzer (Brookhaven, USA); pHs-3C digital pH meter (Shanghai Lei Ci Device Works, China) with a glass electrode (Model E-201-C); and magnetic stirrer (Gongyi Yuhua Instrument Factory, China).
F 4500 spectrofluorophotometer
Manufactured by Hitachi
Sourced in Japan
The F-4500 spectrofluorophotometer is a laboratory instrument designed for the analysis and measurement of fluorescence spectra. It is capable of accurately detecting and quantifying the fluorescence properties of various samples.
Automatically generated - may contain errors
2 protocols using f 4500 spectrofluorophotometer
1
Characterization of Nanoparticle Properties
2
Synthesis and Characterization of TB Nanoparticles
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TB was synthesized according to our previous work (Alifu et al., 2017 (link)). PS-PEG and other chemical regents which not specially mentioned were purchased from Sigma Inc., The deionized water (DI water, 18.2 MΩ cm resistivity) obtained from the system has been utilized in all the experiments Using a Shimadzu UV-3600 UV-vis spectrophotometer to measure UV-vis absorption spectra. Using a Malvern Zetasizer Nano ZS size analyzer to measure dynamic light scattering (DLS) and zeta potential at room temperature.
Fluorescence quantum yield was measured using a Hitachi F4500 spectrofluorophotometer. Rhodamine B (excitation wavelength: 365 nm) was used as a standard to determine the fluorescence quantum yields of the nanoparticles.
Fluorescence quantum yield was measured using a Hitachi F4500 spectrofluorophotometer. Rhodamine B (excitation wavelength: 365 nm) was used as a standard to determine the fluorescence quantum yields of the nanoparticles.
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