Apc cd8α

Manufactured by BD

The APC-CD8α is a fluorochrome-conjugated antibody that binds to the CD8α surface marker on cells. It is a flow cytometry reagent used for the identification and enumeration of CD8+ T cells in biological samples.

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Lab products found in correlation

Facs lsr 2, by BD (2 mentions) B220 pe, by BD (2 mentions) Cd11b pe, by BD (1 mentions) Cd44 apc, by BD (1 mentions) Cd4 pe, by BD (1 mentions) Biotinylated cd28, by BD (1 mentions) Cd8α pe, by BD (1 mentions) Cd3 pe, by BD (1 mentions) Cd25 pe cy7, by BD (1 mentions) Facsaria, by BD (1 mentions) Cd4 fitc, by BD (1 mentions) Foxp3 pe, by Thermo Fisher Scientific (1 mentions) Pe anti mouse igg, by BioLegend (1 mentions) Hrp conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Anti actin antibody, by Cell Signaling Technology (1 mentions) Cd25 percpcy5, by BD (1 mentions) Fitc anti rabbit igg antibody, by BioLegend (1 mentions) Cd4 pe cy7, by BD (1 mentions) Anti cd3 fitc, by BD (1 mentions) Cd86 pe cy7, by BD (1 mentions)

Close spelling variants of this product

CD8α-APC-Cy7 (4 citations) Anti-CD8α-APC (clone 53–6.7, (3 citations) CD8α-APC-H7 (2 citations) CD8α-APC-R700 (2 citations) Anti-CD8α-APC-H7 (clone SK1, (2 citations) APC rat anti-mouse CD8α (2 citations)

4 protocols using apc cd8α

Immunophenotyping Murine Thymus and Spleen

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Single cell suspensions were prepared from both the thymus and spleen of two mice. Red blood cells were removed with RBC lysing solution (1 mM KHCO₃, 0.15 M NH₄Cl, and 0.1 mM Na₂EDTA). Cells were washed twice and resuspended in a master-mix of staining buffer containing optimal concentrations of monoclonal antibody reagents. Samples were analyzed on a FACS LSR II (Becton Dickinson) or sorted with a FACS Aria (Becton Dickinson). Double negative thymic cells were stained with PE-Cy7- CD25 (BD Cat#552880), APC- CD44 (BD Cat#559250), biotinylated- CD28 (BD Cat#553296) (developed secondarily with streptavidin), and a lineage stain [PE-CD3 (BD Cat#555275), monoclonal PE-CD4 (BD Cat#553049), PE-CD8α (BD Cat#553033), PE-B220 (BD Cat#561878), PE-CD11b (BD Cat#553311), PE-NK1.1 (BD Cat#553165)] to remove mature T, B, and NK cells. Double and single positive thymic cells were stained with PE-CD3 (BD Cat#555275), FITC-CD4 (BD Cat#557307), and APC-CD8α (BD Cat#557682). Splenic cells were stained with PE-CD3 (BD Cat#555275), FITC-CD4 (BD Cat#557307), and APC-CD8α (BD Cat#557682). All subsets were also stained with propidium iodide (PI) to exclude dead cells.

Levinson M., Khass M., Burrows P.D, & Schroeder HW J.r. (2020). Replacement of TCR Dβ With Immunoglobulin DH DSP2.3 Imposes a Tyrosine-Enriched TCR Repertoire and Adversely Affects T Cell Development. Frontiers in Immunology, 11, 573413.

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OVA-specific CD8+ T Cell Quantification

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Mice 8–12 weeks of age were immunized intraperitoneally with 100 μg of ovalbumin protein. Spleens were removed 7 days later and stained with monoclonal APC-CD8α (BD Cat#557682) and PE-OVA(257–264) peptide-H-2K(b) (NIH Tetramer Core) and analyzed on a FACS LSR II (Becton Dickinson).

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STAT Signaling Pathway Antibodies Protocol

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Anti-STAT1 antibody (E-23) and anti-STAT2 (07-140) antibody were purchased from Santa Cruz Biotechnologies (Santa Cruz, CA) and Millipore (Billerica, MA), respectively. Anti-STAT2 antibody for immunofluorescence analysis was provided by Dr. Christian Schindler. Anti-STAT3 antibody was provided by Dr. Andrew Larner (Virginia Commonwealth University). Anti-pY701-STAT1, anti-mouse CD16/CD32 (clone 2.4G2), anti-CD3-FITC (Clone: 17A2), CD8α-APC (Clone: 53-6.7), CD4-PE-Cy7 (Clone: RM4-5), B220-PE (Clone: RA3-6B2), Vβ13-FITC (clone: MR12-3) and CD25-Percp-Cy5.5 (Clone: P61) were purchased from BD Biosciences (San Jose, CA). Anti-pY705-STAT3 antibody and anti-Actin antibody were purchased from Cell Signaling (Danvers, MA) and Abcam (Cambridge, MA), respectively. HRP-conjugated secondary antibodies were purchased from Invitrogen (Carlsbad, CA). FITC-anti-Rabbit IgG antibody and PE-anti-Mouse IgG (Biolegend; San Diego, CA) were used for immunocytochemistry and flow cytometry analysis. Foxp3-PE (Clone: FJk-16s) was purchased from eBioscience (San Diego, CA). Recombinant murine IFN-β was provided by Biogen-Idec.

Yue C., Xu J., Estioko M.D., Kotredes K.P., Lopez-Otalora Y., Hilliard B.A., Baker D.P., Gallucci S, & Gamero A.M. (2014). Host STAT2/type I interferon axis controls tumor growth. International journal of cancer. Journal international du cancer, 136(1), 117-126.

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Isolation and Analysis of Tumor-Infiltrating Immune Cells

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Tumors excised on day 26 after injection were homogenized using pestle motor and treated with collagenase type IV (1 mg/mL) and DNase I (100 U/mL) in serum-free RPMI for 30 min at 37 °C with gentle shaking. The cell suspension was passed through a cell strainer (70 µm) and washed with FACS buffer twice. Cells were then incubated with CD16/32 blocking antibody at 1:20 dilution for 10 min and then stained with antibodies for 30 min at room temperature against CD8, CD4, and NK cells. For the staining of CD8 T-cells, CD45-FITC (eBiosciene), H-2K^b OVA tetramer-SIINFEKL-PE (MBL International), CD8α-APC (BD Bioscience), and CD3-PE-CY7 (Biolegend) were used. For CD4 T-cells, CD45-FITC (eBiosciene), CD4-APC (eBioscience), CD3-PE-CY7 (Biolegend) were used, and for DCs, CD45-FITC (eBiosciene), CD11c-PE (Biolegend), and CD86-PE-CY7 (BD Bioscience) were used. For NK cell staining, CD45-FITC (eBiosciene), NK1.1-PE (eBioscience), and CD3-PE-CY7 (Biolegend) were used. In all flow cytometric analyses, antibodies were used at 1:100 dilution, and only DAPI negative live cells were gated out and analyzed.

Son S., Nam J., Zenkov I., Ochyl L.J., Xu Y., Scheetz L., Shi J., Farokhzad O.C, & Moon J.J. (2020). Sugar-Nanocapsules Imprinted with Microbial Molecular Patterns for mRNA Vaccination. Nano letters, 20(3), 1499-1509.

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