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Tom70

Manufactured by Proteintech
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TOM70 is a mitochondrial import receptor that mediates the translocation of precursor proteins into the mitochondrial outer membrane. It functions as a component of the translocase of the outer membrane (TOM) complex, which is responsible for the import of nuclear-encoded proteins into mitochondria.

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Lab products found in correlation

Dihydromyricetin, by Merck Group (1 mentions) Mmp13, by Proteintech (1 mentions) Sirt3 antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 488, by Abcam (1 mentions) Tnf α, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit, by Abcam (1 mentions) Collagenase type 2, by Merck Group (1 mentions) Compound c, by Selleck Chemicals (1 mentions) Confocal fluorescence microscope, by Nikon (1 mentions) Rabbit anti lc3, by Cell Signaling Technology (1 mentions) Fluorescence microscope, by Olympus (1 mentions) Total oxphos cocktail, by Abcam (1 mentions) Cytochrome c, by BD (1 mentions) Bnip3, by Abcam (1 mentions) Mtch2, by Proteintech (1 mentions) Timm23, by Proteintech (1 mentions) Mitofilin, by Proteintech (1 mentions) Hsp60, by Proteintech (1 mentions) Lonp1, by Proteintech (1 mentions) Tom20, by ABclonal (1 mentions)

3 protocols using tom70

1

Dihydromyricetin Inhibits Osteoarthritis

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Dihydromyricetin (purity >98%) and type II collagenase were purchased from Sigma-Aldrich (St Louis, MO, USA). TNF-α was purchased from Peprotech (Texas, USA). Compound C was acquired from Selleckchem (Huston, USA). The primary antibody against BRD4, ADAMTS-5, p53, DRP1, MFN2, NIX, BNIP3, LC3, β-actin and Alexa Fluor 488 or 594 labeled Goat Anti-Rabbit or Anti-Mouse IgG (H+L) second antibody were purchased from Abcam (Cambridge, UK). Primary antibodies of type II collagen, aggrecan, MMP-13, TOM70 were from Proteintech (Rosemont, USA). SIRT3 antibody was from Cell Signaling (Danvers, MA, USA). were from Jackson ImmunoResearch (West Grove, PA, USA).
Wang J., Wang K., Huang C., Lin D., Zhou Y., Wu Y., Tian N., Fan P., Pan X., Xu D., Hu J., Zhou Y., Wang X, & Zhang X. (2018). SIRT3 Activation by Dihydromyricetin Suppresses Chondrocytes Degeneration via Maintaining Mitochondrial Homeostasis. International Journal of Biological Sciences, 14(13), 1873-1882.
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2

Immunofluorescence Analysis of Organelle Markers

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Samples were blocked by 10% goat serum for 1 h at 37°C and probed using primary antibodies mouse anti-SIRT3 (1:200, Santa Cruz Biotechnology), p53 (1:1000, Abcam), Tom70 (1:100, Proteintech), rabbit anti-LC3 (1:100, Cell Signaling Technology) and NIX (Santa Cruz Biotechnology, 1:50) at 4°C overnight. Then slides were incubated with luorescein isothiocyanate- or tetramethyl rhodamine isothiocyanate-conjugated second antibodies for 1 h and labeled with 40, 6-diamidino-2-phenylindole (DAPI, Beyotime) for 5 mins. At last slides were observed in a fluorescence microscope (Olympus Inc., Tokyo, Japan) and a confocal fluorescence microscope (Nikon, Japan). In addition, fluorescence intensity was measured using Image J software 2.1 (Bethesda, MD, USA).
Wang J., Wang K., Huang C., Lin D., Zhou Y., Wu Y., Tian N., Fan P., Pan X., Xu D., Hu J., Zhou Y., Wang X, & Zhang X. (2018). SIRT3 Activation by Dihydromyricetin Suppresses Chondrocytes Degeneration via Maintaining Mitochondrial Homeostasis. International Journal of Biological Sciences, 14(13), 1873-1882.
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3

Multiparametric Mitochondrial Profiling

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BNIP3 (Abcam, ab109362; Abcam, ab10433; Cell signaling, 3769S), NIX (Cell signaling, 12396S), FLAG (Sigma-Aldrich, F1804), HA (Sigma-Aldrich, H6533), ACTIN (Sigma-Aldrich, A2066), TOM70 (Proteintech, 14528-1-AP), TOM20 (ABclonal, A19403), SMAC (Cell signaling, 2954S), MITOFILIN (Proteintech, 10179-1-AP), HSP60 (Cell signaling, 4870S), TIMM23 (Proteintech, 11123-1-AP), SKP1 (Cell signaling, 2156S), CUL1 (Proteintech, 12895-1-AP), Total OXPHOS Cocktail (Abcam, ab110411), Cytochrome C (BD Biosciences, 556433), mCherry (Easybio, BE2026), MTCH2 (Proteintech, 16888-1-AP), BECLIN1 (Cell signaling, 3495T), FIP200 (ABclonal, A14685), TFAM (Abcam, ab131607), LONP1 (Proteintech, 15440-1-AP), VDAC (Cell signaling, 4866S), PARP (Cell signaling, 9542S), Donkey polyclonal anti-Rabbit IgG (H+L), , Donkey polyclonal anti-Mouse IgG (H+L), HRP-conjugated (Jackson ImmunoResearch, 711-035-151), Goat polyclonal anti-Mouse IgG, Fcγ fragment specific, HRP-conjugated (Jackson ImmunoResearch, 115-035-008), Goat polyclonal anti-Mouse IgG, light chain specific, HRPconjugated (Jackson ImmunoResearch, .
Cao Y., Zheng J., Wan H., Sun Y., Fu S., Liu S., He B., Cai G., Cao Y., Huang H., Li Q., Ma Y., Chen S., Wang F, & Jiang H. (2023). A mitochondrial SCF-FBXL4 ubiquitin E3 ligase complex degrades BNIP3 and NIX to restrain mitophagy and prevent mitochondrial disease. The EMBO journal, 42(13).
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