Na2WO4•2H2O (≥99 %), (COOH)2•2H2O (≥99 %), Na2SO4•10H2O (≥99 %), NaClO4•H2O (99.99 %), Na3PO4 (96 %) were purchased from Sigma Aldrich as used as received. 3M HCl was diluted from 37 % HCl solution (Merck). Distilled water was used throughout the experiments.
Naclo4 h2o
Manufactured by Merck Group
Sourced in Belgium
NaClO4 H2O is a chemical compound that consists of sodium, chlorine, oxygen, and hydrogen. It is a crystalline solid that is commonly used as a laboratory reagent. The compound has a molecular formula of NaClO4·H2O and a molar mass of 140.47 g/mol.
Automatically generated - may contain errors
Lab products found in correlation
Na3po4, by Merck Group (1 mentions)
Na2wo4 2h2o, by Merck Group (1 mentions)
Mg no3 2 6h2o, by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Thermo Fisher Scientific (1 mentions)
Lithium chloride (licl), by Thermo Fisher Scientific (1 mentions)
H2so4, by Thermo Fisher Scientific (1 mentions)
1 4 dioxane, by Thermo Fisher Scientific (1 mentions)
Nh4cl, by Thermo Fisher Scientific (1 mentions)
Cacl2 2h2o, by Chem-Lab (1 mentions)
Hclo4, by Thermo Fisher Scientific (1 mentions)
Na2so4, by Chem-Lab (1 mentions)
Lino3, by Merck Group (1 mentions)
Potassium chloride (kcl), by AppliChem (1 mentions)
Mgcl2 6h2o, by Merck Group (1 mentions)
Silicone solution in isopropanol, by Serva Electrophoresis (1 mentions)
Nano3, by Chem-Lab (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Chelex 100 resin, by Bio-Rad (1 mentions)
5 protocols using naclo4 h2o
1
Synthesis of Inorganic Compounds
2
Comprehensive Chemical Reagents Procurement
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MgCl2•6H2O (98%), NaCl (98%), NaClO4•H2O (98%), NaF (99%), Mg(NO3)2•6H2O, KSCN (99%), LiNO3 (99%) and D2O (99.9 atom% D) were obtained from Sigma-Aldrich (Diegem, Belgium). CaCl2•2H2O (99.5%), NaNO3 (99%), Na2SO4 (99%), Ca(NO3)2•4H2O (99%), KNO3 (99%), NH4NO3 (99%) and HNO3 (65%) were purchased from Chem-Lab (Zedelgem, Belgium). KCl (99.5%) and NaI (99.5%) were purchased from AppliChem (Darmstadt, Germany) and LiCl (99%) from Fisher Chemical (Loughborough, UK). CsCl (99%), NaBr (99.5%), NH4Cl (99.5%), H2SO4 (96%), HClO4 (70%), HCl (37%) and 1,4-dioxane (99.9%) were obtained from Acros Organics (Geel, Belgium). The silicone solution in isopropanol was purchased from SERVA Electrophoresis GmbH (Heidelberg, Germany). All chemicals were used as received without further purification.
3
Metalloprotein Purification and Characterization
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Acetonitrile (MeCN), HCl (trace metal grade), NaClO4 H2O, ZnSO4·7H2O, 4-(2-pyridylazo)resorcinol (PAR), and Triton X-100 were from Merck Millipore. Tris(2-carboxyethyl)phosphine hydrochloride (TCEP), tryptone, yeast extract, LB Broth, agar, agarose, isopropyl-β-D-1-thiogalactopyranoside (IPTG) and sodium dodecyl sulfate (SDS) were purchased from Lab Empire. Ampicillin, chloramphenicol, 1,4-dithiothreitol (DTT) and Tris base were from Roth, pTYB21 vector and chitin resin from New England BioLabs, Chelex 100 resin from Bio-Rad, 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt (HEPES), 5,5ʹ-dithiobis-(2-nitrobenzoic acid) (DTNB) from TCI Europe. To eliminate trace metal ion contamination all pH buffers were treated with Chelex 100 resin (Bio-Rad) and degassed over 2 h prior to use. For the culture of E. coli, Luria–Bertani (LB) medium and agar plates were used.
4
Purification and Preparation of Solutions
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Distilled water was further purified by passing it through a mixed bed of ion-exchanger (R3-83002, M3-83006, Bioblock Scientific, Illkirch, France) and activated carbon (Bioblock Scientific ORC-83005) and was de-oxygenated by CO2- and O2-free argon (Sigma Oxiclear cartridge) before use. All the stock solutions were prepared in spectroscopic grade dimethylsulfoxide (DMSO, Bioreagent for Molecular Biology, >>99.9%, Sigma) by weighing solid products using a XA105 Dual Range (0.01/0.1 mg–41/120 g) balance Mettler Toledo (Viroflay, France) and the complete dissolution was achieved using an ultrasonic bath. The ionic strength was maintained at 0.1 M with sodium perchlorate (NaClO4.H2O, Merck, p.a.), and all measurements were carried out at 25.0 ± 0.2 °C. CAUTION! Perchlorate salts combined with organic ligands are potentially explosive and should be handled in small quantities and with the adequate precautions [61 ].
5
Synthesis of Coordination Compounds
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Chemicals were used as purchased without further purification: L-alanine (99.8%, Evonik), L-proline (>99%, Evonik), CuClO 4 •6H 2 O (98%, Sigma-Aldrich), AgClO 4 (97%, Sigma-Aldrich), NaClO 4 •H 2 O (99%, Merck), urea (99.5%, Grüssing).
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