Testosterone propionate

Manufactured by Fujifilm

Sourced in Japan

Testosterone propionate is a laboratory reagent used in various research and analytical applications. It is a form of the testosterone hormone. Testosterone propionate is commonly used as a reference standard or analytical control in the measurement and quantification of testosterone levels in biological samples.

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6 protocols using testosterone propionate

Angiogenic Signaling Pathway Modulation

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6SL were purchased from Carbosynth Ltd. (Berkshire, UK). Antibodies against phosphorylated and total form of VEGFR-2, Akt and ERK were purchased from Cell Signaling Technology (Danvers, MA). Testosterone propionate (TP) was purchased from Wako Pure Chemicals (Tokyo, Japan). Saw palmetto extract (SP) was obtained from Chongkundang Pharm. (Seoul, Korea). Antibodies against cluster of differentiation 31 (CD31; sc-376764) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Kim E.Y., Jin B.R., Chung T.W., Bae S.J., Park H., Ryu D., Jin L., An H.J, & Ha K.T. (2019). 6-sialyllactose ameliorates dihydrotestosterone-induced benign prostatic hyperplasia through suppressing VEGF-mediated angiogenesis. BMB Reports, 52(9), 560-565.

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Testosterone Propionate and Anticancer Effects

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Testosterone propionate (TP) was purchased from Wako Pure Chemicals (Tokyo, Japan). Finasteride was supplied from Merck & Co., Inc. (Kenilworth, NJ, USA). Antibodies against androgen receptor (AR, sc-816), proliferating cell nuclear antigen (PCNA, sc-56), E2F1 (sc-193), Rb (sc-377528), cyclin D1 (sc-753), and β-actin (sc-81178) were obtained from Santa Cruz Biotechnology, Inc. (TX, USA). Antibody against prostate-specific antigen (PSA, Catalog number, PB9259) was purchased from Boster Biological Technology (CA, USA). Loganin (≥97.0%), psoralen (≥99.0%), and isopsoralen (≥95.0%) were procured from Sigma-Aldrich (Seoul, Korea). Morroniside (≥97.0%) was purchased from ChemFaces (Wuhan, Hubei, China). HPLC-grade acetonitrile and water were procured from JT&Baker (Seoul, Korea).

Jin B.R., Kim H.J., Seo J.H., Kim M.S., Lee K.H., Yoon I.J, & An H.J. (2019). HBX-6, Standardized Cornus officinalis and Psoralea corylifolia L. Extracts, Suppresses Benign Prostate Hyperplasia by Attenuating E2F1 Activation. Molecules, 24(9), 1719.

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Molecular Mechanisms of Prostate Cancer Progression

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Testosterone propionate (Cat# 200-17532) was purchased from Wako Pure Chemicals (Tokyo, Japan). Finasteride was procured from Merck & Co., Inc. (NJ, USA). Antibodies against Bcl-2 (Cat# sc-7382), Bcl-xL (Cat# sc-8392), CDK2 (Cat# sc-748), CDK4 (Cat# sc-23896), Cyclin A (Cat# sc-751), Cyclin D1 (Cat# sc-753), Cyclin E (Cat# sc-481), E2F1 (Cat# sc-193), PCNA (Cat# sc-56), p-RB (Cat# sc-377528), and β-actin (Cat# sc-81178) were purchased from Santa Cruz biotechnology, Inc. (Dallas, TX, USA). Antibody against PSA (Cat# PB9259) was procured from Boster Biological Technology (Pleasanton, CA, USA). Horseradish peroxidase conjugated secondary antibodies (Cat# 315-035-003 and 111-035-003) were purchased from Jackson ImmunoResearch Laboratories, Inc. (West Grove, PA, USA). SYBR Green Master Mix (Cat# 4367659) was purchased from Applied Biosystems (Foster City, CA, USA). 5α-reductase 2, CDK2, CDK4, Cyclin A, Cyclin D1, Cyclin E, E2F1, PCNA, PSA, RB, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) oligonucleotide primers were purchased from Bioneer (Daejeon, Korea).

Jin B.R., Cheon S.Y., Kim H.J., Kim M.S., Lee K.H, & An H.J. (2020). Anti-Proliferative Effects of Standardized Cornus officinalis on Benign Prostatic Epithelial Cells via the PCNA/E2F1-Dependent Cell Cycle Pathway. International Journal of Molecular Sciences, 21(24), 9567.

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Testosterone effects on estradiol levels

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Testosterone propionate (Wako, Osaka, Japan) was dissolved in sesame oil (Sigma-Aldrich, Tokyo, Japan) and its concentration was adjusted (20 μg/μl). Testosterone was injected subcutaneously into each group of castrated mice at 1, 10 or 100 μg/g body weight/2 days for 2 weeks from 5 weeks of age, and blood estradiol levels were measured at 7 weeks of age using an ELISA kit (Endocrine Technologies, Inc. USA).

Yabiku K., Nakamoto K, & Tokushige A. (2018). Reintroducing testosterone in the db/db mouse partially restores normal glucose metabolism and insulin resistance in a leptin-independent manner. BMC Endocrine Disorders, 18, 38.

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Inducing Benign Prostatic Hyperplasia in Rats

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Testosterone propionate (Wako Pure Chemical Industries, Ltd., Osaka, Japan) was diluted with corn oil and injected subcutaneously (10 mg/kg) into rats in the BPH, Fina and MSJ groups to induce BPH (9 (link),10 (link)). Finasteride (Merck & Co., Inc.) and MSJ were dissolved in distilled water. Fina and MSJ were orally administered at a dose of 5 and 200 mg/kg, respectively.

Ju J.Y., Chung K.S., Cheon S.Y, & An H.J. (2016). Musulju improves benign prostatic hyperplasia by regulating inflammatory and apoptotic proteins. Molecular Medicine Reports, 14(5), 4692-4698.

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Experimental Prostate Cancer Biomarkers

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Recombinant human TGF-β1 and TGF-β2 proteins were purchased from R&D Systems (Minneapolis, MN, USA). Antibodies against AR, CD68, NOX4, nuclear receptor coactivator 1 (NCOA1), proliferating cell nuclear antigen (PCNA), tumor protein P53 (TP53, also known as P53), transforming growth factor beta (TGFB), actin beta (ACTB), and 8-hydroxy-2-deoxyguanosine (8-OHdG) were purchased from Santa Cruz Biotechnology, Inc. (TX, USA). Boster Biological Technology (CA, USA) provided antibodies against solute carrier family 2 member 9 (SLC2A9, also known as Glut9) and kallikrein-related peptidase 3 (KLK3, also known as PSA). Horseradish peroxidase-conjugated secondary antibodies were purchased from Jackson ImmunoResearch Laboratories, Inc. (PA, USA). SYBR Green Master Mix was purchased from Applied Biosystems (CA, USA). Oligonucleotide primers were purchased from Bioneer (Daejeon, Republic of Korea). Testosterone propionate was purchased from Wako Pure Chemical Industries (Osaka, Japan). Finasteride (Fina) was obtained from Merck & Co., Inc. (NJ, USA).

Jin B.R., Kim H.J., Na J.H., Lee W.K, & An H.J. (2023). Targeting benign prostate hyperplasia treatments: AR/TGF-β/NOX4 inhibition by apocynin suppresses inflammation and proliferation. Journal of Advanced Research, 57, 135-147.

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