E0282

Manufactured by Merck Group

The E0282 is a laboratory equipment product manufactured by Merck Group. It functions as an analytical instrument used for various scientific applications. The core purpose of this equipment is to perform specific measurements and analyses as required by researchers and scientists. No further details can be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Trypsin, by Avantor (1 mentions) Dmem f12, by Sartorius (1 mentions) Dmem f12, by Merck Group (1 mentions) Dispase, by Roche (1 mentions) D0627, by Merck Group (1 mentions) R2625, by Merck Group (1 mentions)

2 protocols using e0282

Isolation of Uterine Luminal Epithelial Cells

Cited 1 time

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Uterine luminal epithelial cells were isolated as previously described (Nallasamy et al., 2012 (link)). The uteri from the estrous mice or PD4 were cut longitudinally, washed in HBSS, incubated in 0.2% (W/V) trypsin (0458, Amresco, Cleveland, USA) and 6 mg/ml dispase (Roche Applied Science,4942078001, Basel, Switzerland) in 4.3 ml HBSS for 1.5 hr at 4℃, 30 min at room temperature, and 10 min at 37℃. After rinsing in HBSS, the epithelial cells were precipitated in 5% BSA in HBSS for 7 min. After the collected epithelial cells were cultured in DMEM/F12 (D2906, Sigma-Aldrich) with 10% heat-inactivated fetal bovine serum (FBS) in a culture plate for 30 min, the unattached epithelial cells were transferred into new culture plates precoated with ECM (1:100, E0282, Sigma-Aldrich) and cultured at 37 °C for 1 hr. Luminal epithelial cells were treated with TNF (10 and 100 ng/ml, 410-MT-010, R＆D systems) in DMEM/F12 with 2% charcoal-treated FBS (cFBS, Biological Industries, Cromwell, CT).

Chen S.T., Shi W.W., Lin Y.Q., Yang Z.S., Wang Y., Li M.Y., Li Y., Liu A.X., Hu Y, & Yang Z.M. (2023). Embryo-derive TNF promotes decidualization via fibroblast activation. eLife, 12, e82970.

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Differentiation of SH-SY5Y Cells

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SH-SY5Y cells (RRID: CVCL_0019) were differentiated as previously described in detail by Shipley et al. [27 ]. Briefly, SH-SY5Y cells below passage 15 were seeded at 5–6 ×10³ cells/cm² in uncoated 6-well plates (day 0 (D0)) and left overnight in growth media. The next day (D1) differentiation was initiated using DMEM/F:12 media with 2.5% hiFBS, 1% p/s, 2 mM glutamine and 10 μM retinoic acid (RA, R2625 Sigma-Aldrich) and media was replaced at D3 and D5. On D7 cells were split 1:1 using 0.05% trypsin-EDTA to uncoated dishes and on D8 hiFBS content was reduced to 1%. On D10 the cells were split 1:1 to extracellular matrix-coated dishes (E0282, Sigma-Aldrich). On D11 media was replaced with the final differentiation media consisting of neurobasal media (21103049, ThermoFisher) with 1X B27 supplement (17504044, ThermoFisher), 20 mM KCl (10697623, Fisher Scientific), 1% p/s, 1X Glutamax, 50 ng/ml brain-derived neurotrophic factor (BDNF, CST-3897S, Peprotech), 2 mM dibutyryl cyclic AMP (db-cAMP, D0627 Sigma-Aldrich) and 10 μM RA. Media was replaced on D14 and D17 and cells were tested and confirmed as fully differentiated on D18 and used for downstream applications as outlined below.

Stage T.B., Mortensen C., Khalaf S., Steffensen V., Hammer H.S., Xiong C., Nielsen F., Poetz O., Svenningsen Å.F., Rodriguez-Antona C, & Kroetz D.L. (2020). P-glycoprotein Inhibition Exacerbates Paclitaxel Neurotoxicity in Neurons and Cancer Patients. Clinical pharmacology and therapeutics, 108(3), 671-680.

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