Total cell lysates (20μg) were resolved by 10% SDS-PAGE, transferred to nitrocellulose membranes, and immunoblotted with antibodies for CD147 (BD), HO-1, p-H2A.X/t-H2A.X, Cyclophilin-A, HMGB1 (Cell Signaling) and β-Actin (Sigma) for loading controls. Immunoreactive bands were identified using an enhanced chemiluminescence reaction (Perkin-Elmer), and visualized by autoradiography.
Cd147
Manufactured by BD
Sourced in United States
CD147 is a cell surface protein that functions as a regulator of various cellular processes. It is expressed on the surface of many cell types and plays a role in cell signaling, adhesion, and migration. The core function of CD147 is to facilitate the transportation of monocarboxylates, such as lactate, across cell membranes.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Merck Group (3 mentions)
Enhanced chemiluminescence reaction, by PerkinElmer (3 mentions)
Adamts1, by Cell Signaling Technology (3 mentions)
Catch and release immunoprecipitation kit, by Merck Group (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
Adamts9, by Thermo Fisher Scientific (2 mentions)
Hmgb1, by Cell Signaling Technology (1 mentions)
Phospho p44 42 erk, by Cell Signaling Technology (1 mentions)
Brdu flow kit, by BD (1 mentions)
P selectin, by BD (1 mentions)
Cd42a, by BD (1 mentions)
Cd40l, by BD (1 mentions)
Pac 1, by BD (1 mentions)
Interleukin 6 (il 6), by BD (1 mentions)
Tnf α, by BD (1 mentions)
Ec800 instrument, by Sony (1 mentions)
Vegfr2, by R&D Systems (1 mentions)
Cd271, by BD (1 mentions)
7 protocols using cd147
1
SDS-PAGE Immunoblot Analysis
2
Western Blot Analysis of CD147, LANA, and ERK
3
Multiparameter Flow Cytometry Analysis
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Preconjugated fluorescent antibodies were from BD Biosciences (San Jose, CA, USA) (CD14–phycoerythrin (PE), P-selectin (CD62P)–PE, immunoglobulin G (IgG1)–PE, CD16–PE-Cy5, CD40L–PE, CD42a–fluorescein isothiocyanate (FITC), PAC-1–FITC, CD147–FITC, TNF-α–FITC, IL-6–FITC, CD61–PerCP, CCR2–Alexa Fluor and BrdU Flow Kit). Anti-CD147 antibody was provided by the Department of Cell Biology, Fourth Military Medical University (Xi’an, China). An anti-IKK beta (IKKβ) antibody was also used (Abcam, Cambridge, UK).
4
Multiplex Immunoblotting for Proteomic Analysis
5
Quantitative Immunoblotting of CD147, ADAMTS1, and ADAMTS9
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Total cell lysates (20 μg) were resolved by 10% SDS–PAGE, transferred to nitrocellulose membranes, and immunoblotted with antibodies for CD147 (BD), ADAMTS1, ADAMTS9 (Cell Signaling) and β-Actin (Sigma) for loading controls. Immunoreactive bands were identified using an enhanced chemiluminescence reaction (Perkin-Elmer), visualized by autoradiography and quantitated using Image-J software.
6
Flow Cytometric Characterization of Stem Cells
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A total of 1 million passage 3 cells were resuspended in 200 µL of PBS containing fluorescein isothiocyanate– or phycoerythrin-coupled antibodies against CD34, CD44, CD45, CD90, CD147, CD271 (BD Biosciences), CD117 (eBioscience), CD105, CD166 (Ancell), and vascular endothelial growth factor receptor–2 (VEGFR-2; R&D Systems) and incubated for 30 minutes at 4°C. The cells were then washed with PBS and resuspended in 350 µL of PBS for analysis. Fluorescein isothiocyanate– or phycoerythrin-coupled nonspecific mouse Immunoglobulin G (BD Biosciences) was used as an isotype control. Cell fluorescence was evaluated via flow cytometry using an EC800 instrument (SONY), and data were also analyzed using the EC800 instrument.9 (link),23 (link),24 (link),32 (link)
7
Multiplex Immunoblotting for Proteomic Analysis
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The following antibodies (100-200 μg/mL) were used in immunoblotting: p-ERK/t-ERK, p-p65/t-p65, ADAMTS1 (Cell Signaling, Cat. #4370, #4695, #3033, #8242, #12897), ADAMTS9 (Thermo, Cat. #PA1-1760), CD147 (BD, Cat. #555961), LANA (ABI, Cat. #13-210-100), vFLIP (Ximbio, Cat. #151778) and HERV-K NP9 (kindly provided by Dr. Friedrich A. Grasser from Universitatsklinikum des Saarlandes, Germany).28 (link) The antibody detecting β-Actin (Cell Signaling, Cat. #4970) was used as the loading control. Immunoprecipitation assays were carried out using the Catch and Release Immunoprecipitation Kit (Milipore).
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