Ly6g peridinin chlorophyll protein percp cy5

Manufactured by BD

Ly6G-peridinin chlorophyll protein (PerCP)-Cy5.5 is a fluorescently-labeled antibody used for flow cytometry analysis. It is a conjugate of the Ly6G antibody and the PerCP-Cy5.5 fluorescent dye, which allows for the detection and quantification of Ly6G-positive cells.

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Lab products found in correlation

Cd11b v450, by BD (2 mentions) Cd45 allophycocyanin apc cy7, by BD (2 mentions) Rat anti mouse cd16 32 clone 93, by BioLegend (2 mentions) Lsr 2 flow cytometer, by BD (2 mentions) Cd45 percp cy5, by BioLegend (1 mentions) Cd4 allophycocyanin apc, by BD (1 mentions) Cd25 fitc, by BD (1 mentions) Cd45 fitc 30 f11, by BioLegend (1 mentions) Cd11b apc fire750, by BioLegend (1 mentions) Cd8a pe, by BioLegend (1 mentions) Cd1d pe, by BioLegend (1 mentions) Cd5 bv421, by BioLegend (1 mentions) Facsaria instrument, by BD (1 mentions) Facsverse instrument, by BD (1 mentions) Cd19 apc, by BD (1 mentions) Ack lysis buffer, by Thermo Fisher Scientific (1 mentions) Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Facsdiva software, by BD (1 mentions)

Close spelling variants of this product

Peridinin chlorophyll protein (PerCP), Ly6G-PerCP-Cy5.5 PerCP-Cy5.5

3 protocols using ly6g peridinin chlorophyll protein percp cy5

Immune Cell Characterization by Flow Cytometry

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The procedure used in this study has been published previously (14 (link),26 (link)). Briefly, the following anti-mouse monoclonal antibodies were purchased from BD Biosciences: Ly6G-peridinin chlorophyll protein (PerCP)-Cy5.5 (1A8; 560602), Ly6C-fluorescein isothiocyanate (FITC) (AL-21; 553104), CD4-allophycocyanin (APC) (RM4-5; 553051), CD25-FITC (7D4; 553071), and CD19-APC (1D3; 550992). The following antibodies were purchased from BioLegend: CD45-FITC (30F-11;103108), CD45-PerCP-Cy5.5 (30F-11;103132), CD45-brilliant violet (BV510) (30F-11;103138), CD11b-APC/Fire750 (M1/70;101262), CD244-Alexa Fluor 647 (2B4;133509), F4/80-phycoerythrin (PE) (BM8;123110), PD-L1-BV421 (10F.9G2;124315), CD8a-APC/Fire 750 (53-6.7;100766), CD8a-PE (53-6.7; 100708), CD127-BV510 (A7R34;135033), CD1d-PE (1B1;123509), and CD5-BV421 (53-7.3;100618). The isolated immune cells were stained in a 96-round-bottom-well plate for 20 min at 4°C and washed with PBS containing 1% bovine serum albumin. Sorting of Ly6G⁺CD244⁺ and Ly6G⁺CD244⁻ cells was conducted on a FACSAria instrument (BD Biosciences). Flow cytometric data were obtained by a FACS Verse instrument (BD Biosciences) and analyzed by FlowJo software (TreeStar, Inc.). CD45-gated cells were analyzed in this study.

Sugita Y., Yamashita K., Fujita M., Saito M., Yamada K., Agawa K., Watanabe A., Fukuoka E., Hasegawa H., Kanaji S., Oshikiri T., Matsuda T., Nakamura T., Suzuki S, & Kakeji Y. (2021). CD244+ polymorphonuclear myeloid-derived suppressor cells reflect the status of peritoneal dissemination in a colon cancer mouse model. Oncology Reports, 45(6), 106.

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Quantifying Nasopharyngeal Neutrophils in Pups

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Neutrophils present in the nasopharynx were quantified as previously described (18 (link)). Briefly, nasal lavage samples from individual pups were pelleted by centrifugation at 500 × g for 5 min. Samples were resuspended in 50 μl ACK lysis buffer (Thermo Fisher Scientific) and incubated for 5 min at room temperature. Afterwards, 200 μl of PBS was added to the samples, and cells were pelleted as described above and resuspended in PBS containing 1% bovine serum albumin (BSA). Samples were stained with a LIVE/DEAD Fixable Aqua dead cell stain kit (Invitrogen, Thermo Fisher Scientific). Next, samples were blocked with a 1:200 dilution of a rat anti-mouse CD16/32 (clone 93; BioLegend). Cells were stained for 30 min at 4°C with fluorophore-conjugated antibodies (diluted 1:150) against the following surface markers: CD11b-V450 (BD), Ly6G-peridinin chlorophyll protein (PerCP)-Cy5.5 (BD), and CD45-allophycocyanin (APC)-Cy7 (BD). Samples were run on a BD LSR II flow cytometer and analyzed with BD FACSDiva software.

Zafar M.A., Hammond A.J., Hamaguchi S., Wu W., Kono M., Zhao L, & Weiser J.N. (2019). Identification of Pneumococcal Factors Affecting Pneumococcal Shedding Shows that the dlt Locus Promotes Inflammation and Transmission. mBio, 10(3), e01032-19.

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Quantifying Nasal Neutrophils in Pups

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To quantify neutrophils, we pelleted nasal lavage samples from individual pups at 500 × g for 5 min and resuspended them in PBS containing 1% bovine serum albumin (BSA). Samples were blocked with a 1:200 dilution of a rat anti-mouse CD16/32 (clone 93; BioLegend). Cells were stained for 30 min at 4° C with fluorophore-conjugated antibodies (diluted 1:150) against the following surface markers: CD11b-V450 (BD), Ly6G-peridinin chlorophyll protein (PerCP)-Cy5.5 (BD), and CD45-allophycocyanin (APC)-Cy7 (BD). Samples were run on BD LSR II flow cytometer and analyzed with BD FACSDiva software.

Zafar M.A., Wang Y., Hamaguchi S, & Weiser J.N. (2017). Host-to-Host Transmission of Streptococcus pneumoniae Is Driven by Its Inflammatory Toxin, Pneumolysin. Cell host & microbe, 21(1), 73-83.

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