Ionpac ag14 precolumn

Bromide (C₀ = 100 mg/L $${\mathrm{Br}}^{-}$$ ) was revealed with a Dionex ICS-1000 IC ion chromatograph (Sunnyvale, CA, USA), with a conductivity cell detector equipped with a Dionex AS-40 Autosampler, a Dionex IonPac™ AG14 precolumn (4 × 50 mm), and a Dionex IonPac™ AS14 IC column with a 4 mm AESR 500 suppressor (Thermo Fisher Scientific, Chelmsford, MA, USA). The eluent phase was prepared with 3.5 mM Na₂CO₃ and 1.0 mM NaHCO₃, with 1.2 mL/min as the flow rate. On the other hand, for the test performed with NaCl (C₀ = 0.02 M), a HandyLab^® 330 conductometer (SI Analytics, Weilheim, Germany) was used to measure the conductivity variation. The data were processed by drawing the F curve (C/C₀) against time.

The content of inorganic ion was determined by ion exchange chromatography (Dionex DX120; Dionex Corporation, Sunnyvale, CA, USA) with a conductivity detector, as reported by D’Imperio et al. [17 (link)]. The content of Na⁺, K⁺, Mg^2+, and Ca²⁺ was determined in 1 g of dried sample, using an IonPac CG12A guard column and an IonPac CS12A analytical column (Dionex Corporation); the elution was performed with 18 mM of methanesulfonic acid (Thermo Scientific™ Dionex™, Waltham, MA, USA). Peaks identification and calibration were performed using the Multi Element IC Standard solution Fluka TraceCERT^®, Supelco^® (Merck KGaA, Darmstadt, Germany). The contents of Cl⁻ and NO₃⁻ were determined in 0.5 g of dried sample using an IonPac AG14 precolumn and an IonPac AS14 separation column (Dionex Corporation). The eluent consisted of 3.5 mmol·L⁻¹ of sodium-carbonate (Thermo Scientific™ Dionex™, USA) and 1.0 mmol·L⁻¹ of sodium-bicarbonate solution (Thermo Scientific™ Dionex™, USA), and 50 mL of the same eluent was used to extract the anions. Inorganic cation content determination was carried out in triplicate. Peaks identification and calibration were performed using the Multi Element IC Standard sol. IC-MAN-18 (6E) of Chem-Lab (Palin Corporation, Elderslie, UK).

The analysis was performed using an ion exchange chromotagraph (Dionex DX120; Dionex Corporation, Sunnyvale, CA, USA) with a conductivity detector, as reported by D’Imperio et al. [67 (link)]. Inorganic anionic content (Cl⁻, NO₃⁻, H₂PO₄⁻, SO₄²⁻) was determined in 0.5 g dried sample ground to powder using an IonPac AG14 precolumn and an IonPac AS14 separation column (Dionex Corporation). All analyses were performed in triplicate.