Mouse anti kit clone ab81

Mouse anti-3BP2 (clone C5), mouse anti-KIT (clone Ab81), mouse anti-BCL2, and mouse anti-CDK2 were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA, USA). Anti-MITF (clone D5G7V) was obtained (was provided by) from Cell Signaling Technology, Inc (Danvers, MA, USA). Mouse anti-β-actin (clone AC-40) and mouse anti-tubulin (DM1A) were purchased from Sigma (St. Louis, MO, USA). Anti-mouse and anti-rabbit IgG peroxidase Abs were acquired and bought from DAKO (Carpinteria, CA, USA) and Biorad (Hercules, CA, USA). ML329 was from Axon Med Chem (Groningen, The Netherlands).

Mouse anti‐SH3BP2 (clone C5 for western blot and C11 for immunofluorescence), mouse anti‐KIT (clone Ab81) for western blot, mouse anti‐PLCγ, rabbit anti‐KIT (clone H300) for immunofluorescence, and rabbit anti‐PDGFRA (clone C20) for immunofluorescence were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Rabbit anti‐pPLCγ (Y783), anti‐AKT, anti‐pAKT 1/2/3S473 (clon193H12), anti‐pKIT(Y703) (clone D12E12), anti‐PDGFRA (polyclonal) for western blot, and anti‐MITF (clone D5G7V) were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Mouse anti‐α‐tubulin (clone DM1A) and anti‐β‐actin (clone AC‐40) were purchased from Sigma (St. Louis, MO, USA). Mouse antiphosphotyrosine (pTyr; clone PY20) was obtained from Zymed Laboratories (Invitrogen Life Technologies, Carlsbad, CA, USA). Anti‐mouse and anti‐rabbit IgG peroxidase Abs were purchased from DAKO (Carpinteria, CA, USA) and Bio‐Rad (Hercules, CA, USA), respectively. Imatinib and bortezomib were purchased from Sigma.