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Sca1 pacific blue

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The Sca1-Pacific Blue is a fluorescent staining reagent used for flow cytometry applications. It is designed to detect and quantify the expression of the Sca1 (Stem Cell Antigen-1) surface marker on cells. The Sca1 antigen is commonly used as a marker for hematopoietic stem and progenitor cells in various species.

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Lab products found in correlation

Cd31 pe cy7, by Thermo Fisher Scientific (2 mentions) Ps473 akt, by Cell Signaling Technology (2 mentions) Pan akt, by Cell Signaling Technology (2 mentions) Pt308 akt, by Cell Signaling Technology (2 mentions) Cd45 fitc, by Thermo Fisher Scientific (2 mentions) Antibodies to irβ, by Cell Signaling Technology (2 mentions) Live dead blue, by Thermo Fisher Scientific (2 mentions) Cd29 alexafluor700, by BioLegend (2 mentions) Cd34 alexafluor647, by BioLegend (2 mentions) Cl316 243, by Bio-Techne (2 mentions) Cd45 pe cy7, by Thermo Fisher Scientific (2 mentions)

2 protocols using sca1 pacific blue

1

Antibody-based Adipose Tissue Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to IRβ, P-T308-Akt, P-S473-Akt and Pan-Akt (all used at 1/1000 dilution) were from Cell Signaling. UCP1 antibody (#10983) was from AbCam (used at 1/100 for IHC and at 1/1000 for western blot). Live/dead Blue (Molecular Probes, L23105), CD31-PE-CY7 (eBioscience, 25-0311)(used at 1/1000), CD45-PE-CY7 (eBioscience, 25-0451) (used at 1/1000), CD29-AlexaFluor700 (Biolegend, 102218)(used at 1/400), CD34-AlexaFluor647 (Biolegend, 119314)(used at 1/200), Sca1-Pacific Blue (BD Bioscience, 560653)(used at 1/1000), CD45-FITC (eBioscience, 11-0451) (used at 1/200) were used for flow cytometry. CL316,243 was from Tocris.
Sanchez-Gurmaches J, & Guertin D.A. (2014). Adipocytes arise from multiple lineages that are heterogeneously and dynamically distributed. Nature communications, 5, 4099.
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2

Antibody-based Adipose Tissue Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Antibodies to IRβ, P-T308-Akt, P-S473-Akt and Pan-Akt (all used at 1/1000 dilution) were from Cell Signaling. UCP1 antibody (#10983) was from AbCam (used at 1/100 for IHC and at 1/1000 for western blot). Live/dead Blue (Molecular Probes, L23105), CD31-PE-CY7 (eBioscience, 25-0311)(used at 1/1000), CD45-PE-CY7 (eBioscience, 25-0451) (used at 1/1000), CD29-AlexaFluor700 (Biolegend, 102218)(used at 1/400), CD34-AlexaFluor647 (Biolegend, 119314)(used at 1/200), Sca1-Pacific Blue (BD Bioscience, 560653)(used at 1/1000), CD45-FITC (eBioscience, 11-0451) (used at 1/200) were used for flow cytometry. CL316,243 was from Tocris.
Sanchez-Gurmaches J, & Guertin D.A. (2014). Adipocytes arise from multiple lineages that are heterogeneously and dynamically distributed. Nature communications, 5, 4099.
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