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Anti cd57 fitc

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Anti-CD57-FITC is a monoclonal antibody conjugated with the fluorescent dye FITC. It is used to detect and quantify CD57-positive cells in flow cytometry applications.

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Lab products found in correlation

Anti cd3 fitc, by BD (2 mentions) Anti hla dr pe, by BD (2 mentions) Anti cd3 alexa 700, by BD (2 mentions) Anti cd3 percp, by BD (2 mentions) Anti cd8 pacblue, by BD (2 mentions) Anti cd20 pecy7, by BD (2 mentions) Anti cd20 apccy7, by BD (2 mentions) Anti cd45 percp, by BD (2 mentions) Anti cd45ra qdot655, by Thermo Fisher Scientific (2 mentions) Anti cd4 pe, by BD (2 mentions) Anti cd4 v450, by BD (2 mentions) Anti cd56 apc, by BD (2 mentions) Anti cd16 fitc, by BD (2 mentions) Anti cd56 pc7, by Beckman Coulter (1 mentions) Anti nkp46 pe, by Beckman Coulter (1 mentions) Anti cd3 apc, by BD (1 mentions) Anti cd45 apc, by Beckman Coulter (1 mentions) Lsr 2 cytometer, by BD (1 mentions) Anti cd45ro ecd, by Beckman Coulter (1 mentions) Live dead fixable violet dead cell stain kit, by Thermo Fisher Scientific (1 mentions)

6 protocols using anti cd57 fitc

1

Comprehensive NK Cell Receptor Analysis

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NK cell receptors were analyzed after staining with appropriate antibody cocktail: anti-CD56-PC7 (N901), anti-NKp30-PE (Z25), anti-NKp44-PE (Z231), anti-NKp46-PE (BAB281), from Beckman Coulter; anti-CD69-FITC (FN50), anti-CD45-APC (J33), anti-CD3-PCP (UCHT1), anti-CD3-FITC (UCHT1), anti-CD3-APC (UCHT1), anti-CD57-FITC (NK-1), anti-Granzyme-B-FITC (GB11), anti-HLA-DR-PE (TU-36), anti-CD8-APC-Cy7 (SK-1), anti-CD107a-FITC (H4A3), anti-Perforin-PE (δ69) from Becton Dickinson; anti-NKG2D-APC (BAT221) from Miltenyi Biotec from R&D Systems.
Shabrish S., Karnik N., Gupta V., Bhate P, & Madkaikar M. (2020). Impaired NK cell activation during acute dengue virus infection: A contributing factor to disease severity. Heliyon, 6(7), e04320.
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2

Polychromatic Flow Cytometry for Assessing Antigen-Specific Immune Responses

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Flow cytometry was performed as described previously [27] (link). Briefly, antigen-specific phenotypes and cytokine secretion profiles were assessed using a qualified polychromatic flow cytometry (PFC) panel. PBMC were co-incubated with peptide pools matched to the GRIN/ENV insert, 1 µg/ml SEB (Sigma-Aldrich, St. Louis, MO, USA) or mock stimuli, CD107a PECy5, BD Golgistop (Becton Dickinson, San Jose, CA, USA) and Brefeldin A (Sigma-Aldrich, Poole Dorset, UK) for 6 hours at 37°C. Cells were stained for viability with LIVE/DEAD® Fixable Violet Dead Cell Stain Kit (Invitrogen, Eugene, OR, USA), and then surface stained by anti-CD4 QD605, anti-CD8 pacific orange, anti-CD19 pacific blue (Invitrogen, Paisley, UK), anti-CD27 APC-H7, anti-CD14 pacific blue, anti-CD57 FITC, anti-B7 integrin PE (Becton Dickinson, San Jose, CA), and anti-CD45RO ECD (Beckman Coulter, High Wycombe, UK). Finally cells were stained intracellularly with anti-CD3 QD655 (Invitrogen, Paisley, UK), anti-IFNγ PE Cy7, anti-TNF-α A700 and anti-IL-2 APC (Becton Dickinson, San Jose, CA, USA) washed and acquired on the same day. At least 750,000 events were acquired on a custom-built BD LSR II cytometer. Data were analyzed and presented using FlowJo (version 8.8 Treestar).
Kopycinski J., Hayes P., Ashraf A., Cheeseman H., Lala F., Czyzewska-Khan J., Spentzou A., Gill D.K., Keefer M.C., Excler J.L., Fast P., Cox J, & Gilmour J. (2014). Broad HIV Epitope Specificity and Viral Inhibition Induced by Multigenic HIV-1 Adenovirus Subtype 35 Vector Vaccine in Healthy Uninfected Adults. PLoS ONE, 9(3), e90378.
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3

Multiparametric Flow Cytometry Analysis

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The fluorochrome labeled monoclonal antibodies (mAbs) anti-CD2-FITC, anti-CD3-Alexa 700, anti-CD3-PerCP, anti-CD4-V450, anti-CD4-PE, anti-CD8-APC Fluor 780, anti-CD8-PacBlue, anti-CD16-FITC, anti-CD20 PECy7, anti-CD19-V450, anti-CD20-APCCy7, anti-CD25-PEcy7, anti- CD28-PE, anti-CD28-FITC, anti-CD38-PE, anti-CD39-FITC, anti-CD45-PerCP, anti-CD56-APC, anti-CD57-FITC, anti-CD197-PECy7, anti-HLA-DR, anti-IgM, anti-IgD, anti-Ki67-FITC, anti-Bcl-2-PE, anti-TNF-α-PE, anti-TNF-α-APC, IFN-γ-PcpCy5.5, and IL-2-PECy7 were purchased from BD Biosciences (Franklin Lakes, NJ). Anti-CD45RA-QDOT655 and anti-CD69-FITC mAb were obtained from Invitrogen (Carlsbad, CA). Anti-CD8-Alexa780, anti-CD27-Alexa Fluor 700, and anti-CD38-eFluor 650NC were obtained from E-bioscience (San Diego, CA). Anti-CD24-PEcy7 and anti-CD279-PE (PD-1) were purchased from Biolegend (San Diego, CA).
Human EBV protein and CMV peptide pool of pp65 sequence consisting of 138 peptides (15 mers with 11 amino acid overlaps) was purchased from JPT Peptide Technologies (Berlin, Germany).
All patients were DSA-free with a calculated panel reactive antibody (PRA) ≤20% at enrollment. Patient samples were assessed for donor-specific alloantibody post-transplantation as described previously (18 (link)).
Xu H., Samy K.P., Guasch A., Mead S.I., Ghali A., Mehta A., Stempora L, & Kirk A.D. (2015). Post Depletional Lymphocyte Reconstitution During Belatacept and Rapamycin Treatment in Kidney Transplant Recipients. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(2), 550-564.
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4

Phenotypic Analysis of Frozen PBMCs

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Frozen PBMCs from HLA-A2 patients (at least 30 × 106 cells at day 0 and 6 days after vaccination) were thawed as previously described.50 Cells were washed twice with ice-cold PBS and were incubated with Human TrueStainFcXTM Fc Receptor Blocking Solution (BioLegend cat. no. 422301) at room temp. for 5 min to block non-specific binding of antibodies. Next, cells were labeled with recommended amounts of anti-CD3-FITC (BD Biosciences cat no. 555916), anti-CD19-FITC (BD Bioscience cat no. 555412), anti-CD20-FITC (BD Bioscience cat no. 555622), anti-CD57-FITC (BD Bioscience cat no. 555619), anti-CD56-FITC (BD Bioscience cat no. 562794), anti-HLA-DR-PE (BD Bioscience cat no. 555812), anti-CD14-PerCP-Cy5.5 (BioLegend cat no. 301823) and anti-CD11b-APC (BioLegend cat no. 301310) at 4°C in the dark for 30 min. Labeled cells were washed twice with PBS, resuspended in 0.4 ml PBS, and immediately analyzed on a FACSCanto flow cytometer. Data acquisition was performed using BD FACSDiva v.6.1.2.
Kwiatkowska-Borowczyk E., Czerwińska P., Mackiewicz J., Gryska K., Kazimierczak U., Tomela K., Przybyła A., Kozłowska A.K., Galus Ł., Kwinta Ł., Dondajewska E., Gąbka-Buszek A., Żakowska M, & Mackiewicz A. (2018). Whole cell melanoma vaccine genetically modified to stem cells like phenotype generates specific immune responses to ALDH1A1 and long-term survival in advanced melanoma patients. Oncoimmunology, 7(11), e1509821.
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5

Multiparametric Flow Cytometry Protocol

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Monoclonal antibodies (mAbs) anti-CD3-Alexa-700, anti-CD3-PerCP, anti-CD4-V450, anti-CD4-PE, anti-CD8-PacBlue, anti-CD16-FITC, anti-CD20-PECy7, anti-CD20-APCCy7, anti-CD31-FITC, anti-CD45-PerCP, anti-CD56-APC, anti-CD57-FITC, anti-CD57-BV605, anti-CD127 (IL-7Rα)-APC, and anti-CCR7-PECy7 were purchased from BD Biosciences (Franklin Lakes, NJ). Anti-CD45RA-QDOT655 was obtained from Invitrogen (Carlsbad, CA). Anti-CD8-APC-eFluor-780 and anti-PD1-PE were purchased from BioLegend (San Diego, CA). Recombinant human IL-7 and IL-7 enzyme-linked immunosorbent assay (ELISA) kits were purchased from R&D Systems (Minneapolis, MN).
Xu H., Bendersky V.A., Brennan T.V., Espinosa J.R, & Kirk A.D. (2017). IL-7 Receptor Heterogeneity as a Mechanism for Repertoire Change during Post-Depletional Homeostatic Proliferation and its Relation to Costimulation Blockade–Resistant Rejection. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 18(3), 720-730.
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6

Comprehensive Phenotyping of CMV-specific T Cells

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PBMCs thawed and cultured overnight in IMDM (Lonza, Basel, Switzerland), supplemented with 10% human serum (HS, Sigma-Aldrich) were labelled with anti-CD8-Alexa Fluor700 (Clone: RPA-T8, BD Biosciences, New Jersey, USA), anti-CD3-PE-Cy7 (Clone: UCHT1), anti-CD45RA-PerCP Cy5.5 (Clone: HI100), anti-CD27-BV605 (Clone: 0323), anti-CD28-APC(Clone: CD28.2), anti-PD-1-BV421(Clone: EH12.2H7) from Biolegend (San Diego, USA), anti-CCR7-PECF594 (Clone: 150603), anti-CD57-FITC (Clone:NK-1, BD Biosciences) along with AlexaFluor750 labeled live/Dead stain (Life Technologies, Carlsbad, USA). Live/dead and CCR7 staining were performed at 37°C for 15 mins followed by staining with the rest of the antibodies at 4°C for 30 mins. Subsequently, cells were washed, resuspended in PBS and acquired on BD™ LSR II (BD Biosciences, San Jose, USA). The phenotypic analysis was performed on FlowJo version 7.6.5 (Treestar, Ashland, USA). For phenotyping of CMV specific T cells, PE labeled tetramer HLA/CMV epitope complex (HLA-A*01:01 CMV pp50: VTEHDTLLY; HLA-A*02:01 pp65-NLVPMVATV; HLAA*24:02 pp65-QYDPVAALF; HLA-B*07: 02 pp65-TPRVTGGGAM; HLA-B*08:01 IE1-ELRRKMMYM; HLA-B*35:01 pp65-IPSINVHHY, MBL International, Woburn, USA) was included in the panel with the above mentioned antibodies.
Verma K., Ogonek J., Varanasi P.R., Luther S., Bünting I., Thomay K., Behrens Y.L., Mischak-Weissinger E, & Hambach L. (2017). Human CD8+ CD57- TEMRA cells: Too young to be called "old". PLoS ONE, 12(5), e0177405.
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