Mouse c peptide elisa

Manufactured by ALPCO

Sourced in United States

The Mouse C-peptide ELISA is an in vitro diagnostic test used to quantitatively measure the levels of C-peptide in mouse samples. C-peptide is a byproduct of insulin production and can be used as a marker for insulin secretion. The ELISA kit provides the necessary reagents and materials to perform the analysis.

Automatically generated - may contain errors

Lab products found in correlation

Mouse insulin elisa, by Mercodia (3 mentions) Mouse insulin elisa, by ALPCO (2 mentions) Mouse leptin elisa, by Crystal Chem (1 mentions) Mouse adiponectin elisa, by Adipogen (1 mentions) Glycogen colorimetric assay kit 2, by Abcam (1 mentions) Cholesterol e kit, by Fujifilm (1 mentions) Luminex technology, by Merck Group (1 mentions) Nefa hr 2 link kit, by Fujifilm (1 mentions) Humalog, by Eli Lilly (1 mentions) Mouse proinsulin elisa, by Mercodia (1 mentions) Rat c peptide elisa, by ALPCO (1 mentions) Human ultrasensitive insulin elisa kit, by ALPCO (1 mentions) Human proinsulin elisa, by Mercodia (1 mentions) Contour blood glucose monitoring system, by Bayer (1 mentions) Nod cg prkdcscid il2rgtm1wjl szj nsg mice, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

Mouse C-peptide ELISA kit (12 citations) ELISAs (7 citations) C-peptide (5 citations) C-peptide (ELISA, (3 citations) Mouse C-peptide (2 citations)

8 protocols using mouse c peptide elisa

Metabolic Biomarker Analysis in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum insulin (Mouse Insulin ELISA, Mercodia, Uppsala, Sweden), leptin (Mouse Leptin ELISA, Crystal Chem, Downers Grove, USA), C-peptide (Mouse C-peptide ELISA, ALPCO, Salem, USA), chemerin (Mouse Chemerin Quantikine ELISA, R&D Systems, Minneapolis, USA), adiponectin (Mouse Adiponectin ELISA, AdipoGen, San Diego, USA) and MCP-1 (Mouse/rat CCL2/JE/MCP-1 Quantikine ELISA, R&D Systems, Minneapolis, USA) levels were detected by ELISA according to the manufacturer’s protocol. For the analysis of free fatty acids (FFA), total cholesterol and triglycerides serum concentrations in fasting mice, an automatic chemical analyzer was used, provided by the Institute of Laboratory Medicine and Clinical Chemistry, Medical Department, University of Leipzig.

Zieger K., Weiner J., Kunath A., Gericke M., Krause K., Kern M., Stumvoll M., Klöting N., Blüher M, & Heiker J.T. (2017). Ablation of kallikrein 7 (KLK7) in adipose tissue ameliorates metabolic consequences of high fat diet-induced obesity by counteracting adipose tissue inflammation in vivo. Cellular and Molecular Life Sciences, 75(4), 727-742.

+ Open protocol

+ Expand

Metabolic Biomarker Assessment in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adiponectin (Adiponectin mouse ELISA, Adipogen Life Sciences, Liestal, Switzerland), C-peptide (Mouse c-peptide ELISA, Alpco, Salem, NH), insulin (mouse insulin ELISA, Mercodia AB, Uppsala, Sweden), and leptin (Mouse/Rat Leptin, R&D systems Europe, Ltd., Abingdon, UK) were measured according to manufacturer’s protocol from mouse serum for both groups.
Liver samples were analyzed for glycogen content (Glycogen colorimetric Assay Kit II, Biovision, Inc., Milpitas, CA) and triglycerides (LabAssay™, Wako Pure Chemical Industries, Ltd., Osaka, Japan).

Berger C., Heyne H.O., Heiland T., Dommel S., Höfling C., Guiu-Jurado E., Lorenz J., Roßner S., Dannemann M., Kelso J., Kovacs P., Blüher M, & Klöting N. (2021). A novel compound heterozygous leptin receptor mutation causes more severe obesity than in Leprdb/db mice. Journal of Lipid Research, 62, 100105.

+ Open protocol

+ Expand

Glucose Homeostasis Evaluation in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were fasted for 4 h during the light period to ensure a postprandial state for blood sampling. Fasting and glucose-stimulated (2 g/kg) insulin secretion was assessed, as well as blood glucose response to intraperitoneal injection of glucose (2 g/kg) or insulin analogue (0.75 U/kg of Humalog; Eli Lilly, Indianapolis, IN, USA). Plasma insulin was measured with a mouse insulin ELISA (Alpco Diagnostics, Salem, NH, USA) and C-peptide was measured in a subset of 27-week-old mice with a mouse C-peptide ELISA (Alpco Diagnostics). In plasma from 40-week-old mice, we measured total cholesterol (Cholesterol E kit; Wako Chemicals, Richmond, VA, USA), triacylglycerols (Serum Triacylglycerol kit; Sigma-Aldrich, St Louis, MO, USA) and NEFA levels (NEFA-HR(2 (link)) kit; Wako Chemicals), as well as leptin, resistin, interleukin 6, glucose-dependent insulinotropic polypeptide (GIP), peptide YY and glucagon, using a mouse magnetic bead panel assay utilising Luminex technology (Millipore, St Charles, MO, USA).

Templeman N.M., Clee S.M, & Johnson J.D. (2015). Suppression of hyperinsulinaemia in growing female mice provides long-term protection against obesity. Diabetologia, 58(10), 2392-2402.

+ Open protocol

+ Expand

Fasted vs. Fed Submandibular Bleeds

Check if the same lab product or an alternative is used in the 5 most similar protocols

Submandibular bleeds of either overnight-fasted or fed animals were done in the morning or following an oGTT. Insulin was analyzed with mouse insulin ELISA (Mercodia). Proinsulin was analyzed with mouse proinsulin ELISA (Mercodia). C peptide was analyzed with mouse C-peptide ELISA (ALPCO). All ELISAs were performed according to the manufacturer’s instructions.

Kleiner S., Gomez D., Megra B., Na E., Bhavsar R., Cavino K., Xin Y., Rojas J., Dominguez-Gutierrez G., Zambrowicz B., Carrat G., Chabosseau P., Hu M., Murphy A.J., Yancopoulos G.D., Rutter G.A, & Gromada J. (2018). Mice harboring the human SLC30A8 R138X loss-of-function mutation have increased insulin secretory capacity. Proceedings of the National Academy of Sciences of the United States of America, 115(32), E7642-E7649.

+ Open protocol

+ Expand

Quantifying Insulin Production via C-peptide

Check if the same lab product or an alternative is used in the 5 most similar protocols

In this study C-peptide concentrations as a marker for insulin production, since the half-life of C-peptide is longer than that of insulin. Thereby, by measuring C-peptide we can focus on de novo synthesis of insulin and not just circulating insulin that might also be released/taken up by cells. Peripheral blood samples obtained after each experiment were centrifuged and the retrieved serum samples were stored at −20°C until analyzed. C-peptide concentrations were measured using a commercially available ELISA kit (mouse C-peptide ELISA; Alpco immunoassays, Salem, NH, USA).

Toonen E.J., Laskewitz A.J., van Dijk T.H., Bleeker A., Grefhorst A., Schouten A.E., Bastiaanssen E.A., Ballak D.B., Koenders M.I., van Doorn C., van der Vleuten M.A., van Lierop M.J., Groen A.K, & Dokter W.H. (2014). Glucose Kinetics in the Collagen-Induced Arthritis Model: An All-in-One Model to Assess Both Efficacy and Metabolic Side Effects of Glucocorticoids. PLoS ONE, 9(9), e98684.

+ Open protocol

+ Expand

Measuring Serum C-Peptide in Rodent Islet Transplants

Check if the same lab product or an alternative is used in the 5 most similar protocols

A 30-μl blood sample (random C-peptide) was collected via submandibular vein prick for mouse recipients and tail vein prick for rat recipients and assayed for serum human C-peptide using a Stellux Chemiluminescence Human C Peptide ELISA kit (ALPCO, Salem, NH) for mice receiving human islets and with Mouse C Peptide ELISA (ALPCO, Salem, NH) for mice receiving MIN6 clusters and with Rat C Peptide ELISA for rats receiving rat islets. An IPGTT was conducted as follows: Mice or rats were fasted overnight before IPGTT, and then a 30-μl blood sample was collected via submandibular vein prick for mice or tail vein prick for rats just before IPGTT. Mice or rats were then intraperitoneally injected with 2 g per kilogram of glucose. Blood glucose measurements were taken via tail vein at time 0, 10, 20, 30, 45, 60, 90, and 120 min after administration. A 30-μl blood sample was collected at 0 min (before glucose administration) and 30 min after glucose administration via submandibular vein prick (stimulated C-peptide). Blood samples were assayed for C-peptide as described above.

Stock A.A., Gonzalez G.C., Pete S.I., De Toni T., Berman D.M., Rabassa A., Diaz W., Geary JC J.r., Willman M., Jackson J.M., DeHaseth N.H., Ziebarth N.M., Hogan A.R., Ricordi C., Kenyon N.S, & Tomei A.A. (2022). Performance of islets of Langerhans conformally coated via an emulsion cross-linking method in diabetic rodents and nonhuman primates. Science Advances, 8(26), eabm3145.

+ Open protocol

+ Expand

Evaluating Stem Cell Transplant Efficacy

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Animal studies were performed in accordance with Washington University IACUC. Mice were randomly designated for STZ treatment and transplantation groups. Mouse number per group was selected to allow for statistical significance based on our prior studies (15 (link), 17 (link), 18 (link)). Surgical procedures and follow up studies were performed by unblinded individuals. Male 7 wk old NOD.Cg-Prkdc^scidIl2rg^tm1Wjl/SzJ (NSG) mice were purchased from Jackson Laboratories, rendered diabetic with injection of 45 mg/kg STZ (R&D) for 5 d, with diabetes confirmed after 8 d. Anaesthetized mice were injected with 5x10⁶ WS4^unedit stage 6 cells, 5x10⁶ WS4^corr stage 6 cells, 5x10⁶ (4000 IEQ) islet cells, or saline under the kidney capsule. Islet transplantation was performed in a separate cohort. Animals were monitored up to 6 months. Blood glucose was measured with a Contour Blood Glucose Monitoring System (Bayer). Glucose tolerance and in vivo GSIS assays were performed by fasting mice for 4 hr and injecting with 2 g/kg glucose. Serum hormones were quantified using Human Ultrasensitive Insulin ELISA kit (ALPCO Diagnostics), Mouse C-peptide ELISA (ALPCO Diagnostics), and human proinsulin ELISA (Mercodia). 12-wk after transplantation, live nephrectomy was performed on 2 anaesthetized transplanted mice.

Maxwell K.G., Augsornworawat P., Velazco-Cruz L., Kim M.H., Asada R., Hogrebe N.J., Morikawa S., Urano F, & Millman J.R. (2020). Gene-edited human stem cell-derived β cells from a patient with monogenic diabetes reverse preexisting diabetes in mice. Science translational medicine, 12(540), eaax9106.

+ Open protocol

+ Expand

Insulin Secretion in Fasted Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Fasting insulin levels were measured in animals fasted for 5 h following indicated interventions by blood sampling via tail vein and determined by mouse insulin ELISA (Alpco) following manufacturer’s instructions. C-Peptide levels were determined by mouse c-peptide ELISA (Alpco). Insulin secretion following glucose challenge was determined at 0 and at 15 min following oral gavage of glucose (2 mg/g body weight).

Watt K.I., Henstridge D.C., Ziemann M., Sim C.B., Montgomery M.K., Samocha-Bonet D., Parker B.L., Dodd G.T., Bond S.T., Salmi T.M., Lee R.S., Thomson R.E., Hagg A., Davey J.R., Qian H., Koopman R., El-Osta A., Greenfield J.R., Watt M.J., Febbraio M.A., Drew B.G., Cox A.G., Porrello E.R., Harvey K.F, & Gregorevic P. (2021). Yap regulates skeletal muscle fatty acid oxidation and adiposity in metabolic disease. Nature Communications, 12, 2887.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!