Anti cd163

Manufactured by Bioss Antibodies

Sourced in United States, China

Anti-CD163 is an antibody that binds to the CD163 protein, which is expressed on the surface of certain immune cells. CD163 is a scavenger receptor that plays a role in the clearance of hemoglobin-haptoglobin complexes. The Anti-CD163 antibody can be used in research applications to identify and study cells expressing the CD163 protein.

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Lab products found in correlation

Anti b220, by Thermo Fisher Scientific (1 mentions) Anti cd8a, by BD (1 mentions) Anti cd19, by Thermo Fisher Scientific (1 mentions) Anti f4 80, by BioLegend (1 mentions) Anti cd3e, by Thermo Fisher Scientific (1 mentions) Ra3 6b2, by Thermo Fisher Scientific (1 mentions) Anti cd64, by BioLegend (1 mentions) Anti cd115, by Thermo Fisher Scientific (1 mentions) Anti ccr3, by BioLegend (1 mentions) Anti mhcii, by BioLegend (1 mentions) Anti cd11c, by BioLegend (1 mentions) Anti cd68, by Bio-Rad (1 mentions) Anti cd11b, by Thermo Fisher Scientific (1 mentions) Facscanto 2, by BD (1 mentions) Facsdiva, by BD (1 mentions) Histofine simple stain kit, by Nichirei Biosciences (1 mentions) Anti bcl 2, by Santa Cruz Biotechnology (1 mentions) Anti flag m2, by Merck Group (1 mentions) Paeoniflorin, by Yuanye Bio-Technology (1 mentions) Anti timp 1, by Bioss Antibodies (1 mentions)

3 protocols using anti cd163

Multicolor Flow Cytometry Panel for Immune Cell Profiling

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Cells were stained with the following fluorochrome-conjugated antibodies: anti-B220 (eBioscience, clone RA3-6B2), anti-CD3e (eBioscience, clone 145-2C11), anti-CD8a (BD, clone 53-6.7), anti-CD11b (eBioscience, clone M1/70), anti-CD11c (Bio Legend, clone N418), anti-CD14 (Bio Legend, clone SA14-2), anti-CD19 (eBioscience, clone eBio1D3), anti-CD64 (Bio Legend, clone X54-5/7.1), anti-CD68 (AbD Serotec, clone FA-11), anti-CD163 (Bioss, polyclonal), anti-CD115 (eBioscience, clone AF598), anti-CCR3 (Bio Legend, clone J073E5), anti-F4/80 (Bio Legend, clone CI:A3-1), anti-FPR-1 (Bioss, polyclonal), anti-MHC II (Bio Legend, clone M5/114.15.2), anti-MR (AbD Serotec, clone MR5D3) and anti-PILRa (R&D Systems, polyclonal). Fc receptors were blocked with 1.5mg/ml human IgG (Privigen). Dead cells were excluded using the Hoechst 33342 dye. Only events that appeared single in forward-scatter width were analyzed. The gating strategy is shown in Supplementary Figure 7. A FACSCanto II and FACSDiva software (BD) were used for flow cytometry and data were analyzed with FlowJo software (TreeStar).

Franken L., Klein M., Spasova M., Elsukova A., Wiedwald U., Welz M., Knolle P., Farle M., Limmer A, & Kurts C. (2015). Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates. Scientific Reports, 5, 12940.

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Immunohistochemical Analysis of Tumor Markers

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Formaldehyde-fixed, paraffin-embedded tumor tissues were stained with hematoxylin and eosin (H&E) using standard techniques. Immunohistochemistry was performed using primary antibodies in conjunction with the Histofine Simple Stain kit (Nichirei, Tokyo, Japan). The following primary antibodies were used: Anti-FLAG M2 (Sigma, Burlington, MA, USA), anti-AE1/AE3 (DAKO, Santa Clara, CA, USA), anti-BCL2 (Santa Cruz Biotechnology, Dallas, TX, USA), anti-TLE1 (Santa Cruz Biotechnology), and anti-CD163 (Bioss Antibodies, Woburn, MA, USA).

Tanaka M., Homme M., Yamazaki Y., Ae K., Matsumoto S., Subramanian S, & Nakamura T. (2020). Cooperation between SS18-SSX1 and miR-214 in Synovial Sarcoma Development and Progression. Cancers, 12(2), 324.

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Anti-Inflammatory and Anti-Fibrotic Mechanisms

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Paeoniflorin was purchased from Yuanye Bio-Technology (Shanghai, China), and lificiguat (YC-1) from SelleckChem (Houston, TX, USA). CCl 4 was provided by Tianjin Chemical (Tianjin, China), and CoCl 2 was obtained from Makclin Biochemical Co. Ltd. The antibodies targeting MMP2, P65, p-P65, p-I B / , and p-IKK were acquired from Cell Signaling Technologies (Danvers, MA, USA). The anti-MMP9 and anti-TGF-antibodies were from Abcam (Cambridge, MA, UK), and the anti-HIF-, anti-TNF-, and anti-IL-6 antibodies were from Affinity Biosciences (Jiangsu, China). The anti-F4/80, anti-iNOS, anti-ARG-1, anti-CD163, anti-IL-10, and anti-TIMP-1 antibodies were from Bioss Biotechnology (Beijing, China).
In addition, antibodies specific for -SMA, GAPDH, and -actin, as well as the HRP-linked goat, anti-rabbit, and goat antirat IgG antibodies were obtained from Servicebio (Wuhan, China). The rabbit immunohistochemistry kit was provided by ZSGB Biotechnology (Beijing, China), and Alexa Fluor 488/594 goat anti-rat/rabbit IgG H&L was purchased from Invitrogen (CA, USA).

Liu Y., He C.Y., Yang X.M., Chen W.C., Zhang M.J., Zhong X.D., Chen W.G., Zhong B.L., He S.Q, & Sun H.T. (2023). Paeoniflorin Coordinates Macrophage Polarization and Mitigates Liver Inflammation and Fibrogenesis by Targeting the NF-[Formula: see text]B/HIF-1α Pathway in CCl₄-Induced Liver Fibrosis. The American journal of Chinese medicine, 51(5).

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