96‐well plates (NUNC, Maxisorp) were coated either with polyclonal α‐mouse IgM or IgG antibody (SouthernBiotech), respectively, and blocked with buffer containing 1% BSA. Dilutions of mouse IgM or IgG antibodies (SouthernBiotech) were used as standard. The concentration of IgM or IgG antibodies in the sera was determined by detection with alkaline phosphatase‐labeled α‐mouse IgM or IgG (Southern Biotech), respectively. P‐nitrophenylphosphate (Genaxxon) in diethanolamine buffer was added, and data were acquired at 405 nm using a Multiskan FC ELISA plate reader (Thermo Scientific). For detection of α‐HEL antibodies, sera were adjusted to an IgM concentration of 500 μg/ml and applied in dilution steps of 1:3 to plates coated with 10 μg/ml HEL. For detection of α‐TNP IgG antibodies, sera were adjusted to an IgG concentration of 1 μg/ml and applied in dilution steps of 1:3 to plates coated with 1 μg/ml TNP‐conjugated BSA.
α mouse igm or igg
Manufactured by Southern Biotech
α‐mouse IgM or IgG is a laboratory reagent used to detect and quantify mouse immunoglobulin M (IgM) or immunoglobulin G (IgG) in biological samples. It functions as a specific antibody that binds to the respective mouse immunoglobulin isotypes, enabling their identification and measurement.
Automatically generated - may contain errors
Lab products found in correlation
α mouse igm or igg antibody, by Southern Biotech (2 mentions)
P nitrophenyl phosphate, by Thermo Fisher Scientific (2 mentions)
Multiskan fc elisa plate reader, by Thermo Fisher Scientific (2 mentions)
Calf thymus dna, by Rockland Immunochemicals (1 mentions)
Lps from escherichia coli, by Merck Group (1 mentions)
2 protocols using α mouse igm or igg
1
Quantification of Mouse Antibody Isotypes
2
Analysis of Blimp-1 Impact on Splenic B Cell IgM and Autoantibody Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mature splenic B cells from the indicated genotypes were cultured in the presence of 2.5 μg/mL LPS from Escherichia coli (0111:B4; Sigma-Aldrich) for 1.5 days and subjected to retroviral transduction either with pMIG (EV) or pMIG-Blimp-1, respectively. The supernatants were collected 5 days after transduction and used for α-IgM and α-dsDNA-IgM-ELISA.
For the α-IgM and α-IgG ELISAs 96-well plates (NUNC, maxisorp) were coated with polyclonal α-mouse IgM or IgG antibody (SouthernBiotech) and blocked with buffer containing 1% BSA. Dilutions of α-mouse IgM/IgG (SouthernBiotech) were used as standard. The concentration of IgM and IgG in the supernatants was determined by detection with alkaline-phosphatase-labeled α-mouse IgM or IgG (Southern Biotech), respectively. P-nitrophenylphosphate (Genaxxon) in diethylamine buffer was added and data were acquired at 405 nm using a Multiskan FC ELISA plate reader (Thermo Scientific). To determine the content of autoreactive IgM antibodies, IgM-concentration-adjusted cell culture supernatants were applied to plates coated with calf thymus DNA (Rockland Immunochemicals). The detection and development was performed as described for the α-IgM ELISA.
For the α-IgM and α-IgG ELISAs 96-well plates (NUNC, maxisorp) were coated with polyclonal α-mouse IgM or IgG antibody (SouthernBiotech) and blocked with buffer containing 1% BSA. Dilutions of α-mouse IgM/IgG (SouthernBiotech) were used as standard. The concentration of IgM and IgG in the supernatants was determined by detection with alkaline-phosphatase-labeled α-mouse IgM or IgG (Southern Biotech), respectively. P-nitrophenylphosphate (Genaxxon) in diethylamine buffer was added and data were acquired at 405 nm using a Multiskan FC ELISA plate reader (Thermo Scientific). To determine the content of autoreactive IgM antibodies, IgM-concentration-adjusted cell culture supernatants were applied to plates coated with calf thymus DNA (Rockland Immunochemicals). The detection and development was performed as described for the α-IgM ELISA.
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