Log In Sign up
The largest database of trusted experimental protocols

Lysogeny broth

Manufactured by BD
Visit Supplier
Sourced in United States

Lysogeny broth (LB) is a nutrient-rich growth medium commonly used in microbiology and molecular biology laboratories for the cultivation of various bacterial species. It provides essential nutrients, such as amino acids, vitamins, and carbohydrates, to support the growth and proliferation of bacterial cultures.

Automatically generated - may contain errors

Lab products found in correlation

Tetracycline, by Merck Group (3 mentions) Bhi broth, by BD (2 mentions) Dulbecco modified eagle medium (dmem), by Lonza (2 mentions) Htb 44, by American Type Culture Collection (2 mentions) Chloramphenicol, by Merck Group (2 mentions) Chamberlain s defined media, by Teknova (2 mentions) Agarose, by Thermo Fisher Scientific (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Casamino acid, by Thermo Fisher Scientific (1 mentions) Chloroform, by Thermo Fisher Scientific (1 mentions) Phenylalanine arginine β naphthylamide, by Merck Group (1 mentions) 2 nitrophenyl β d galactopyranoside, by Thermo Fisher Scientific (1 mentions) Mueller hinton broth (mhb), by BD (1 mentions) Triton x 100, by Merck Group (1 mentions) Cholesterol, by Merck Group (1 mentions) 1 2 dipalmitoyl sn glycero 3 phosphocholine dppc, by Avanti Polar Lipids (1 mentions) Micropipette puller, by Sutter Instruments (1 mentions) N octadecyltrimethoxysilane, by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) 2 methoxy polyethyleneoxy propyl trimethoxysilane, by Gelest (1 mentions)

Close spelling variants of this product

Lysogeny Broth (LB) medium (9 citations) Difco lysogeny broth (LB; (4 citations) Lysogeny Broth (LB; (4 citations) Lysogeny Broth (LB) broth (3 citations) Lysogeny Broth (LB) agar (2 citations)

37 protocols using lysogeny broth

1

Reagents for Antimicrobial Susceptibility Testing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Gentamicin, agarose, chloroform and casamino acids were obtained from Fisher Scientific (Ottawa, ON, Canada). Erythromycin was purchased from Caledon Laboratories LTD (Georgetown, WA, Canada). Phenylalanine-Arginine-β-Naphthylamide, cholesterol and Triton X-100 were purchased from Sigma Aldrich (Oakville, ON, Canada). The compound 2-Nitrophenyl-β-d-galactopyranoside was obtained from Thermo Fisher Scientific (Ottawa, ON, Canada). DPPC (1,2-Dipalmitoyl-sn-glycero-3-phosphocholine) was obtained from Avanti Polar Lipids Inc. (Alabaster, AL, USA). Mueller–Hinton broth (MHB) and Lysogeny broth (LB) and agar were purchased from Becton Dickinson (Francklin Lakes, NJ, USA) and Becton Dickinson Microbiology Systems (Oakville, ON, Canada), respectively. ABt medium and Z buffer were prepared as described previously [47 (link),51 (link)].
Gbian D.L, & Omri A. (2021). The Impact of an Efflux Pump Inhibitor on the Activity of Free and Liposomal Antibiotics against Pseudomonas aeruginosa. Pharmaceutics, 13(4), 577.
+ Open protocol
+ Expand
2

Bacterial Growth in Minimal Media

Check if the same lab product or an alternative is used in the 5 most similar protocols
Overnight cultures were grown in Lysogeny Broth (LB), Becton Dickinson (Franklin Lakes, NJ, USA), diluted in 0.85% saline and introduced into in liquid or into 0.35% Bacto agar with Davis minimal salts [47 ] supplemented with 0.2mg/ml glucose as the sole and limiting carbon source. The liquid cultures were maintained with 10 ml of the medium in 50 ml flasks. For the 3-d colony experiments, 3ml of bacteria suspended in soft agar were put into the wells of 6-well Costar Macrotiter plates, set in a tray with distilled water to reduce the rate of evaporation.
Shao X., Mugler A., Kim J., Jeong H.J., Levin B.R, & Nemenman I. (2017). Growth of bacteria in 3-d colonies. PLoS Computational Biology, 13(7), e1005679.
+ Open protocol
+ Expand
3

Microfluidic Polymersome Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Microfluidic devices were fabricated following procedures outlined by Weitz et al. (Ho et al. 2008 (link)) Briefly, two glass capillaries (O.D. 1 mm) were tapered to diameters of approximately 20 and 200 µm using a Flaming/Browning micropipette puller (Sutter P-97). The tip of the smaller (injection) capillary was coated with n-octadecyltrimethoxysilane (Sigma-Aldrich) while the tip of the larger (collection) capillary was coated with 2-[methoxy(polyethyleneoxy)propyl]trimethoxysilane (Gelest, Inc.). The capillaries were coaxially aligned within a square glass capillary tube (O.D. 1.05 mm) with an intercapillary distance of 100 µm. Three 20-gauge syringe tips were epoxied over the square capillary termini to allow injection of each liquid phase with syringe pumps via polyethylene tubing (O.D. 1.32 mm). For polymersome synthesis, three liquid phases were prepared: An aqueous solution comprised of Lysogeny broth (LB, Becton-Dickson), an organic polymer solution containing 2.5 mg/mL of diblock copolymer poly(ethylene glycol)-b-poly(D,L-lactic acid) (mPEG-PDLLA, 5000 MW mPEG and 50,000 MW PDLLA, Polyscitech) and 1.0 mg/mL of poly(lactic acid) (PLA, 15,000 MW, Polysciences) dissolved in a 2:1 ratio of toluene : chloroform, and an aqueous solution comprised of 10 wt% poly(vinyl alcohol) (PVA, 13,000–23,000 MW, 98% hydrolyzed, Sigma-Aldrich) dissolved by heating in deionized water.
Barlow J., Gozzi K., Kelley C.P., Geilich B.M., Webster T.J., Chai Y., Sridhar S, & van de Ven A.L. (2016). High throughput microencapsulation of B. subtilis in semi-permeable biodegradable polymersomes for selenium remediation. Applied microbiology and biotechnology, 101(1), 455-464.
+ Open protocol
+ Expand
4

Preparation of GFP-labeled Salmonella Typhimurium

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Green-fluorescent protein (GFP)-labeled S. enterica serovar Typhimurium SL1334 strain (Kroupitski et al., 2009 (link); Gu et al., 2013a (link)) was used throughout the study. Bacterial culture was prepared and stored in Lysogeny broth (LB; Becton Dickinson, United States) supplemented with glycerol at −70°C, as described (Kroupitski et al., 2009 (link)). For each experiment, fresh culture was prepared by plating the bacteria on a new LB plate supplemented with 100mg/ml streptomycin and 10mg/ml gentamicin for 24h at 37°C. Two to three single colonies were as inoculated into LB broth devoid of NaCl (LBNS) and grown at 37°C with shaking (150rpm) for 18–20h. Cultures were washed twice with sterile saline (0.85% NaCl) by centrifugation at 2,700g for 10min, and the final pellet was resuspended in sterile saline. Bacterial concentration was determined by plating × 10-fold serial dilutions on LB agar supplemented with the two antibiotics.
Chahar M., Kroupitski Y., Gollop R., Belausov E., Melotto M, & Sela-Saldinger S. (2021). Determination of Salmonella enterica Leaf Internalization Varies Substantially According to the Method and Conditions Used to Assess Bacterial Localization. Frontiers in Microbiology, 12, 622068.
+ Open protocol
+ Expand
5

Characterization of S. aureus Transposon Mutants

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The strains used in this study are shown in Table 1. Bursa aurealis transposon (Tn) insertion mutations encoding functionally non-redundant TCA- and urea cycle enzymes (Figure 1) in USA300-JE2 were obtained from the Nebraska Transposon Mutant Library (NTML, www.beiresources.org) [16 (link)]. Parental strains UAS391, UAS391-EryS (erythromycin resistance cured UAS391), and JE2 are all MRSA belonging to the highly virulent and widespread clonal lineage, USA300. These, as well as Tn insertion mutants, were routinely grown on Brain-Heart infusion (BHI; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) supplemented with 0.1% D(+)-glucose monohydrate (Merck Millipore, Billerica, MA, USA) and BHI Bacto™ agar (Becton, Dickinson and Company, USA) for biofilm, transduction and complementation experiments. Lysogeny broth (LB; Becton, Dickinson and Company, USA) was used for Escherichia coli. For the Tn-carrying S. aureus transductants with the erythromycin resistance marker ermB, 5 or 10 µg/mL erythromycin (Sigma-Aldrich®, Merck KGaA, St. Louis, MO, USA) was supplemented to the growth medium.
De Backer S., Sabirova J., De Pauw I., De Greve H., Hernalsteens J.P., Goossens H, & Malhotra-Kumar S. (2018). Enzymes Catalyzing the TCA- and Urea Cycle Influence the Matrix Composition of Biofilms Formed by Methicillin-Resistant Staphylococcus aureus USA300. Microorganisms, 6(4), 113.
+ Open protocol
+ Expand
6

Bacterial Strain Identification and Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols
The strains used in this study are listed in Supplementary Table 1. Salmonella strains were identified by universal primers (F: 5′-GTGGCGGACGGGTGAGTAA-3′, R: 3′-GTGTGACCTTGACTTCGTGCC-5′), serotyped by Salmonella antiserum (Ningbo Tianrun Biological Pharmaceutical Co., Ltd., Zhejiang, China), and cultured in Lysogeny broth (LB) (Becton, Dickinson and Company, United States) at 37°C shaker or on Salmonella-Shigella (SS) selective agar (Hopebio, China) at 37°C incubator. E. coli and Klebsiella pneumoniae strains were cultured in LB broth at 37°C shaker. S. aureus and E. faecalis were cultured in brain heart infusion (BHI) broth (Becton, Dickinson and Company, United States) at 37°C shaker.
Wang X., Xing Y., Ji Y., Xi H., Liu X., Yang L., Lei L., Han W, & Gu J. (2022). The Combination of Phages and Faecal Microbiota Transplantation Can Effectively Treat Mouse Colitis Caused by Salmonella enterica Serovar Typhimurium. Frontiers in Microbiology, 13, 944495.
+ Open protocol
+ Expand
7

Characterization of Clinical MRSA and Staphylococcus Isolates

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Methicillin-resistant S. aureus strain obtained from ATCC (strain number 33592) was tested. Staphylococcus epidermidis and S. aureus clinical isolates (n = 57) were obtained from the Microbiology Laboratories of University of Padova and University of Urbino and subjected to morphological, biochemical, and molecular typing as described elsewhere (Hall et al., 2013 (link)). The strains were grown in Lysogeny broth (LB) (Becton Dickinson).
Bernabè G., Dal Pra M., Ronca V., Pauletto A., Marzaro G., Saluzzo F., Stefani A., Artusi I., De Filippis V., Ferlin M.G., Brun P, & Castagliuolo I. (2021). A Novel Aza-Derivative Inhibits agr Quorum Sensing Signaling and Synergizes Methicillin-Resistant Staphylococcus aureus to Clindamycin. Frontiers in Microbiology, 12, 610859.
+ Open protocol
+ Expand
8

Bacterial Growth and Preparation for ITO Slide

Check if the same lab product or an alternative is used in the 5 most similar protocols
Bacteria were grown in Lysogeny Broth (LB, Becton-Dickenson, Hunt Valley, MD, USA) in shaking liquid culture (200 RPM), to mid-log phase, at 37°C. Pseudomonas aeruginosa (Pa) strain ATCC 31482 and Escherichia coli (Ec) strain ATCC 25922 were used in these studies. The same LB formulation, with the addition of 1.5% (w/v) Bacto agar (Becton-Dickenson), was used for growth on solid media. One microliter of bacterial suspensions in PBS (Pa and Ec at OD600 : 1.7±0.2, 1.0±0.1, 0.1±0.01, and 0.01±0.003), spotted on the conductive side of an ITO slide and the droplet was left to dry under ambient laboratory conditions prior to matrix application.
Yang H., Jackson S.N., Woods A.S., Goodlett D.R., Ernst R.K, & Scott A.J. (2020). Streamlined Analysis of Cardiolipins in Prokaryotic and Eukaryotic Samples Using Norharmane Matrix by MALDI-MSI. Journal of the American Society for Mass Spectrometry, 31(12), 2495-2502.
+ Open protocol
+ Expand
9

Antibiotics Purchase and Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ampicillin (AMP) and kanamycin (KAN) were purchased from Meiji Seika Pharma (Tokyo, Japan). Cefepime (FEP) was purchased from Bristol-Myers Squibb (New York, NY, USA). Colistin (CST), minocycline (MIN), polymyxin B (PMB), and tetracycline (TET) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). AMK and tigecycline (TGC) were purchased from Wako Pure Chemical Industries (Osaka, Japan). Sitafloxacin (STX) was purchased from Cimic CMO (Tokyo, Japan). Cefozopran (ZOP), doxycycline (DOX), and IPM were generously provided by Takeda Pharmaceutical (Osaka, Japan), Pfizer (New York, NY, USA), and Banyu Pharmaceutical (Tokyo, Japan), respectively. CIP, sparfloxacin (SPX), and meropenem (MEM) were generously provided by Sumitomo Dainippon Pharma (Osaka, Japan). Lysogeny broth (LB; Miller), LB ager (Miller), and Muller Hinton Broth II (MHB II) were purchased from Becton Dickinson (Franklin Lakes, NJ, USA). Cation-adjusted MHB II was prepared by adding CaCl2 and MgCl2 (Wako Pure Chemical Industries) at the final concentrations of 50 mg/L Ca2+ and 25 mg/L Mg2+ to autoclaved MHB II. Saline was prepared as 0.9% NaCl (Wako Pure Chemical Industries).
Nishida S, & Ono Y. (2018). Comparative analysis of the pathogenicity between multidrug-resistant Acinetobacter baumannii clinical isolates: isolation of highly pathogenic multidrug-resistant A. baumannii and experimental therapeutics with fourth-generation cephalosporin cefozopran. Infection and Drug Resistance, 11, 1715-1722.
+ Open protocol
+ Expand
10

Isolation and Characterization of P. aeruginosa Phages

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The bacterial strains used in this study are listed in Supplementary Table S1. P. aeruginosa strain PaTun is a clinical isolate obtained from the Hospital Hedi Shaker in Sfax, Tunisia, and was used as a host strain for phage isolation. A collection of 140 clinical and environmental P. aeruginosa isolates from the collection of the Queen Astrid Military Hospital (Brussels, Belgium) [30 (link)] was also used (Supplementary Table S1). All strains were grown in Lysogeny Broth (LB, Becton Dickinson, Erembodegem, Belgium) medium at 37 °C aerobically.
Akremi I., Merabishvili M., Jlidi M., Haj Brahim A., Ben Ali M., Karoui A., Lavigne R., Wagemans J., Pirnay J.P, & Ben Ali M. (2022). Isolation and Characterization of Lytic Pseudomonas aeruginosa Bacteriophages Isolated from Sewage Samples from Tunisia. Viruses, 14(11), 2339.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!