Bone marrows were isolated from femur and tibia of IkkαWt/Wt and IkkαAA/AA congenic donors. Recipient mice were irradiated with a lethal dose of 9 Gy to get rid of the immune cells and were injected with 4 × 106 cells in 100 µl PBS in the tail vein. The transplanted mice were given broad-spectrum antibiotic (Ciprobay; Bayer) at a concentration of 1 mg/ml for 2 wk in drinking water. After 8 wk in total, the mice were treated with DSS and sacrificed on day 9. Colons were collected and stained for H&E.
Ciprobay
Manufactured by Bayer
Sourced in United States, Germany
Ciprobay is a laboratory equipment product manufactured by Bayer. It is designed for use in scientific and clinical research settings. The core function of Ciprobay is to provide a reliable and precise means of conducting various laboratory tests and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Stable glutamine, by Harvard Bioscience (2 mentions)
Automacs, by Miltenyi Biotec (2 mentions)
Mouse pan t cell isolation kit, by Miltenyi Biotec (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Glutamax supplement, by Thermo Fisher Scientific (1 mentions)
Human pan t cell isolation kit, by Miltenyi Biotec (1 mentions)
Ficoll gradient, by Harvard Bioscience (1 mentions)
Fetal calf serum (fcs), by PAN Biotech (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
5 protocols using ciprobay
1
Bone Marrow Transplantation in Colitis Mouse Model
2
Purification and Culture of Murine T Cells
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gp91phox−/− mice were a kind gift from Dr. Katrin Breitbach (Friedrich Loeffler Institute of Medical Microbiology, Ernst-Moritz-Arndt University, Greifswald). Wild type control C57BL/6JBom mice were obtained from Taconics. Mice were kept in pathogen-free conditions (SPF). Spleens from mice were passed through a fine mesh filter (BD Falcon) to obtain a single-cell suspension. T cells were purified by non-T cell depletion using mouse pan T-cell isolation kit and AutoMACS (all from Miltenyi Biotec). Purity of T cells, determined by flow cytometry, was routinely more than 96%. The cells were cultured at 106 cells/ml, 37°C and 5% CO2 in RPMI 1640 medium supplemented with stable glutamine (Biochrom AG), 10% fetal calf serum PAN Biotech), 2 μg/ml ciprobay (Bayer) and 50 μM β-mercaptoethanol (Sigma Aldrich).
3
Ins and Isoproterenol Effects on Cardiomyocytes
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Cor.4U® cardiomyocytes were seeded at a density of 30,000 cells per well. From day 3 on the cells were cultured in Iscove’s Basal Medium containing 1 % GlutaMAX supplement (both Life Technologies, Carlsbad, CA, USA) and 2 µg/ml Ciprobay (Bayer, Leverkusen, Germany). Treatment with 500 nM of the designated Ins or analogue or 100 nM isoproterenol was started at day 4 after seeding. Impedance of each well was measured with the RTCA Cardio xCELLigence Analyser [24 (link)] (Acea Biosciences, San Diego, CA, USA) during the whole experiment. Beating-rate and cell index were analysed by RTCA cardio software (Acea Biosciences, San Diego, CA, USA).
4
Purification of Human T Cells
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Peripheral blood mononuclear cells were isolated by Ficoll gradient (Biochrom AG) centrifugation of heparinized blood collected from healthy volunteers. T cells were further purified by non-T cell depletion using human pan T-cell isolation kit and AutoMACS (all from Miltenyi Biotec). Purity of T cells, determined by flow cytometry, was routinely more than 96%. T cells were cultured at 106 cells/ml, 37°C and 5% CO2 in RPMI 1640 medium supplemented with stable glutamine (Biochrom AG), 10% fetal calf serum (PAN Biotech) and 2 μg/ml ciprobay (Bayer).
5
Culturing Etoposide-Resistant Cell Lines
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Cells employed in this study were obtained from the cell and tumor bank of the University of Duisburg Essen, Medical School. Wildtype (WT) and etoposide-resistant cells were cultured in DMEM medium (Invitrogen, Karlsruhe, Germany) containing 10 % heat-inactivated fetal calf serum (FCS) and 15 μg/ml Ciprobay (Bayer AG Wuppertal, Germany).
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