SGC 7901 cells were purchased from the Cell Bank of the Institute of Life Science, Chinese Academy of Sciences, Shanghai, China. Cells were cultured in Dulbecco's modified Eagle medium (DMEM, HyClone) supplemented with 10% fetal bovine serum (FBS) (HyClone). NaHS, DL-propargylglycine (PPG), and hydroxylamine (HA) were from Sigma (St. Louis, MO, USA). Anti-CSE and anti-CBS monoclonal antibodies were from Abnova (Taiwan). The antibodies for Bax, cytochrome C, caspase 3, cyclin D1, p21, p27, and actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-MMP-2 and anti-MMP-9 antibodies were from Thermo Scientific (Waltham, USA).
Dl propargylglycine ppg
Manufactured by Merck Group
Sourced in United States
DL-propargylglycine (PPG) is a laboratory reagent used in research applications. It is a synthetic amino acid derivative. PPG functions as an inhibitor of cystathionine gamma-lyase, an enzyme involved in the metabolism of sulfur-containing amino acids.
Automatically generated - may contain errors
Lab products found in correlation
Hydroxylamine ha, by Merck Group (1 mentions)
Caspase 3, by Santa Cruz Biotechnology (1 mentions)
Cytochrome c, by Santa Cruz Biotechnology (1 mentions)
Cyclin d1, by Santa Cruz Biotechnology (1 mentions)
μ slide angiogenesis, by Ibidi (1 mentions)
Aperio imagescope software, by Leica (1 mentions)
Growth factor reduced matrigel, by BD (1 mentions)
Buthionine sulfoximine bso, by Cayman Chemical (1 mentions)
Z val ala asp ome ch2f z vad fmk, by Peptide Institute (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Cystathionine β synthase cbs, by Proteintech (1 mentions)
Hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
32p atp, by PerkinElmer (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
L cysteine, by Merck Group (1 mentions)
4 protocols using dl propargylglycine ppg
1
Hydrogen Sulfide Regulation of Gastric Cancer
2
Matrigel Tube Formation Assay for Angiogenesis
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A Matrigel tube formation assay was performed using HMEC-1 cells to study angiogenic capacity of microvascular endothelial cells. Ten μl growth factor-reduced Matrigel (BD Biosciences) was allowed to polymerize at 37 °C for 30 minutes in Angiogenesis μ-slides (Ibidi GmbH). HMEC-1 cells were plated on the Matrigel surface (5000 cells/well in 50 μl serum-free medium). To inhibit CSE activity, DL-propargylglycine (PPG, Sigma-Aldrich) was used. Tube formation was performed in presence of CSE inhibitor PPG using various concentrations (0, 1, 2, 5 and 10 mmol/L) in order to inhibit CSE activity. To inhibit CSE mRNA expression two different siRNAs (single or in combination) were used as described above and tube formation was analyzed 40 hours after transfection. Slow release H2S compound GYY4137 (kindly provided by M. Whitemann, Exeter, UK) was used as an H2S donor in a concentration of 300 μmol/L37 (link). After 8 hours, images were taken from 5 selected areas per well in a standardized way. The total number of branching points in the 5 images was determined using Aperio Imagescope software (Aperio Technologies). The tube formation assay was repeated three times, in which each replicate was performed in triplicate.
3
Pharmacological Modulation of Glutathione Metabolism
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CDDP, SAS, and DL-propargylglycine (PPG) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Buthionine sulfoximine (BSO) was purchased from Cayman Chemical (Ann Arbor, MI, USA). Z-Val-Ala-Asp(OMe)-CH2F (Z-VAD-FMK) was purchased from Peptide Institute, Inc. (Osaka, Japan). Anti-xCT antibody (ab37185) was purchased from Abcam (Cambridge, UK). Anti-cleaved poly (ADP-ribosyl) polymerase (PARP) antibody (#9541) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Anti-GCLC antibody (GTX16315) was purchased from Gene Tex, Inc. (San Antonio, TX, USA). Anti-cystathionine gamma lyase (CGL) antibody (12217-1-AP) was purchased from Proteintech (San Antonio, TX, USA).
4
Investigating Cystathionine Metabolism Enzymes
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Cystathionine‐β‐synthase (CBS) and cystathionine‐γ‐lyase (CSE) antibodies were purchased from Proteintech (Chicago, IL); [32P]ATP was purchased from PerkinElmer (Cambridge, MA); HRP‐conjugated secondary antibodies were obtained from Cell Signaling Technology (Danvers, MA); PVDF membranes were obtained from Millipore (Billerica, MA); Effectene Transfection Reagent, QIAEX®II was from Qiagen (Germantown, MD); culture medium (Dulbecco's modified Eagle's medium) was from Fisher Scientific (Ashville, NC);l ‐Cysteine and dl ‐propargylglycine (PPG) were from Sigma (St. Louis, MO). All other reagents were from Sigma (St. Louis, MO).
Rabbits (New Zealand white male) weighing 4–5 lbs were purchased from RSI Biotechnology (Clemmons, NC), and mice (male C57BL/6 strain) were purchased from Jackson Laboratories (Bar Harbor, ME). Rabbits and mice were acclimatized at the facility administered by the Division of Animal Resources, Virginia Commonwealth University. The Institutional Animal Care and Use Committee of Virginia Commonwealth University approved all the procedures conducted. Colons from normal human subjects were obtained from a nonprofit organization known as National Disease Research Interchange (NDRI, Philadelphia, PA) that provides human organs and tissue. The studies involving human tissues are approved as exempt from VCU Institutional Review Board.
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Cystathionine‐β‐synthase (CBS) and cystathionine‐γ‐lyase (CSE) antibodies were purchased from Proteintech (Chicago, IL); [32P]ATP was purchased from PerkinElmer (Cambridge, MA); HRP‐conjugated secondary antibodies were obtained from Cell Signaling Technology (Danvers, MA); PVDF membranes were obtained from Millipore (Billerica, MA); Effectene Transfection Reagent, QIAEX®II was from Qiagen (Germantown, MD); culture medium (Dulbecco's modified Eagle's medium) was from Fisher Scientific (Ashville, NC);
Rabbits (New Zealand white male) weighing 4–5 lbs were purchased from RSI Biotechnology (Clemmons, NC), and mice (male C57BL/6 strain) were purchased from Jackson Laboratories (Bar Harbor, ME). Rabbits and mice were acclimatized at the facility administered by the Division of Animal Resources, Virginia Commonwealth University. The Institutional Animal Care and Use Committee of Virginia Commonwealth University approved all the procedures conducted. Colons from normal human subjects were obtained from a nonprofit organization known as National Disease Research Interchange (NDRI, Philadelphia, PA) that provides human organs and tissue. The studies involving human tissues are approved as exempt from VCU Institutional Review Board.
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