After stimulation, EoL-1 cells were washed in FACS buffer (phosphate-buffered saline (PBS) with 2% fetal bovine serum (FBS)) and incubated for 45 min at 4°C in the dark with the following antibodies: APC antihuman CD63 (BioLegend), APC antihuman CD69 (BioLegend), APC antihuman CD23 (BioLegend), and APC antihuman FcεR1α (BioLegend). Cell staining was assessed by flow cytometry on an Accuri C6 Flow Cytometer (BD Biosciences). FACS analysis was performed with FlowJo software (Tree Star Inc., Ashland, OR, USA).
Apc antihuman cd23
Manufactured by BioLegend
Sourced in United States
APC anti-human CD23 is a flow cytometry reagent that binds to the CD23 cell surface antigen expressed on B cells, B-cell chronic lymphocytic leukemia cells, and activated monocytes. It is conjugated to the fluorochrome allophycocyanin (APC).
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using apc antihuman cd23
1
Flow Cytometry Analysis of Immune Markers
2
Multiparameter Flow Cytometry Immunophenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell staining with PE anti-human Siglec-8 (BioLegend), PE anti-human CD (CD80) (BioLegend), APC anti-human FcεR1α (BioLegend), APC anti-human CD86 (BioLegend), APC anti-human CD23 (BioLegend), APC anti-human CD63 (BioLegend), PE anti-human PDL1 (BioLegend), PE anti-human IL-5 receptor alpha (IL-5Rα; R&D Systems) were assessed by flow cytometry on an Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA) and data analyzed with FlowJo software (Tree Star Inc., Ashland, OR, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!