Matrix metalloproteinase 9 mmp 9

Type A gelatin (300 Bloom), Epigallocatechin gallate (EGCG), 2,2′-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide, matrix metalloproteinase-9 (MMP-9, EC 3.4.24.35), and benzalkonium chloride (BAC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). N-isopropylacrylamide (NIPAAm), from Acros Organics (Geel, Belgium), was purified by recrystallization from n-hexane. FNC Coating Mix (i.e., a fibronectin/collagen mixture) was obtained from Athena ES (Baltimore, MD, USA). Keratinocyte serum-free medium (KSFM) was purchased from Gibco-BRL (Grand Island, NY, USA). All the other chemicals were of reagent grade and used as received without further purification.

The conventional amino acids, Fmoc-Ala, Fmoc-Lys(Boc)-OH, Fmoc-Arg(Pbf)-OH, Fmoc-Gly, Fmoc-His(Trt)-OH, Fmoc-Leu, Fmoc-Ser(tBu)-OH, Fmoc-Thr(OtBu)-OH, Fmoc-Trp(Boc)-OH, Fmoc-Tyr(tBu)-OH, Fmoc-Asn(Trt)-OH, Fmoc-Ile-OH, Fmoc-Phe-OH, Fmoc-Cys(Trt)-OH, Fmoc-Pro-OH, were acquired from GL Biochem Ltd. (Shanghai, China). N,N′-diisopropylcarbodiimide (DIC), Oxyma pure, 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate (HATU), N,N-Diisopropylethylamine, triisopropylsilane (TIS), 1,1,1,3,3,3-Hexafluoro-2-propanol (HFIP), nonadecanoic acid (C19) were purchased from Sigma-Aldrich (Italy). Rink amide p-methylbenzhydrylamine (MBHA) resin, Fmoc-L-Lys(Mtt)-OH, piperidine, and trifluoroacetic acid (TFA) were purchased from Iris Biotech GmbH (Marktredwitz, Germany). Anhydrous solvents [N,N-dimethylformamide (DMF) and dichloromethane (DCM)], Doxo-EMCH, matrix metalloproteinase-9 (MMP-9) and dialysis tubing benzoylated, Nile Red, and Thioflavin T were purchased from Sigma-Aldrich (Milan, Italy). Silver nitrate (AgNO₃), and trisodium citrate were obtained from Sigma-Aldrich (Luzais, Saint-Quentin-Fallavier, France). Ultrapure water was produced using a Barnstead EASYpure RoDi system (Thermo Fisher Scientific, Villebon sur Yvette, France). All chemicals and reagents were of analytical grade and used as received.