Phospho p53 ser15 9286

Cell lysates were prepared and western blots were performed as previously described [13] (link). We performed 2 parallel samples in an independent experiment under ELF-EMF exposure, and the cell lysates of 2 parallel samples were pooled into a vial to perform the immunoblotting. Three independent experiments were performed. The primary antibodies used for western blotting were: ATM (2873), Chk2 (3440), phospho-Chk2 Thr68 (2661), p53 (2527) and phospho-p53 Ser15 (9286) from Cell Signaling Technology (Beverly, MA); phospho-ATM Ser1981 (2152-1) from Epitomics (Burlingame, CA); and β-Actin (sc-47778) from Santa Cruz (Santa Cruz, CA). The horseradish peroxidase conjugated secondary antibodies were: anti-mouse IgG HRP-linked antibody (7076) and anti-rabbit IgG HRP-linked antibody (7074) from Cell Signaling Technology (Beverly, MA).

Primary antibodies against the β5 (sc-55009) proteasome subunit, NQO1 (sc-16464), Beclin 1 (BECN1) (sc-11427), Clusterin (CLU) (sc-6419), HSP27 (sc-13132), HSP70 (sc-59570), and GAPDH (sc-25778), as well as the horseradish peroxidase-conjugated secondary antibodies, were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). The primary antibodies against 53BP1 (4937), phospho-Histone H2A.X^Ser139 (γH2A.X) (9718) and phospho-p53^Ser15 (9286) were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). The antibody against SQSTM1/p62 (BML-PW9860) was purchased from Enzo Life Science, Inc. (Farmingdale, NY, USA).