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Pdgf aa antibody

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The Pdgf-aa antibody is a laboratory reagent used for the detection and quantification of the platelet-derived growth factor subunit AA (PDGF-AA) in various biological samples. The antibody specifically binds to the PDGF-AA protein, allowing for its identification and measurement through various analytical techniques.

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PDGF-AA (27 citations) Pdgf-aa blocking antibody (2 citations)

2 protocols using pdgf aa antibody

1

Cell Migration Assay with Macrophage Conditioned Media

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3T3 mouse fibroblasts were seeded at 100% confluency into a 96-well plate with a central cell-free detection zone in the center of each well (Platypus technologies). 100 μl of conditioned media of macrophage cultures (48h culture in RPMI containing 10% FBS) were added per well. Pdgf-aa antibody (catalog number 07–1436, Millipore) was added at concentration 0.05 μg/ml. Cells were imaged via bright field microscopy at time points 0h and 24h. Image processing and migration quantification was done using ImageJ.
Aran D., Looney A.P., Liu L., Wu E., Fong V., Hsu A., Chak S., Naikawadi R.P., Wolters P.J., Abate A.R., Butte A.J, & Bhattacharya M. (2019). Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage. Nature immunology, 20(2), 163-172.
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2

Cell Migration Assay with Macrophage Conditioned Media

Check if the same lab product or an alternative is used in the 5 most similar protocols
3T3 mouse fibroblasts were seeded at 100% confluency into a 96-well plate with a central cell-free detection zone in the center of each well (Platypus technologies). 100 μl of conditioned media of macrophage cultures (48h culture in RPMI containing 10% FBS) were added per well. Pdgf-aa antibody (catalog number 07–1436, Millipore) was added at concentration 0.05 μg/ml. Cells were imaged via bright field microscopy at time points 0h and 24h. Image processing and migration quantification was done using ImageJ.
Aran D., Looney A.P., Liu L., Wu E., Fong V., Hsu A., Chak S., Naikawadi R.P., Wolters P.J., Abate A.R., Butte A.J, & Bhattacharya M. (2019). Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage. Nature immunology, 20(2), 163-172.
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