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Anti ded1p

Manufactured by US Biological
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Anti-Ded1p is a lab equipment product designed to detect and measure the presence of the Ded1p protein in biological samples. It functions as a detection tool for this specific protein, providing researchers with a means to analyze its expression and activity levels.

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Lab products found in correlation

Anti hexokinase, by US Biological (2 mentions)

2 protocols using anti ded1p

1

Yeast Ded1p Western Blot Analysis

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Lysates from yeast strain A14201 at vegetative phase and stage 11 (ndt80 release timecourse) were prepared as described.15 (link) After loading equal amounts of protein on a 10% NEXT gel, denaturing gel electrophoresisand transfer to a PVDF membrane, Western blotting was performedwith anti-Ded1p (Rabbit; 1:5000) and anti-Hexokinase(Rabbit;1:10,000; US Biological) antibodies. Chemiluminescencewas quantified by Imagequant software. Hexokinaseserved as a loading control for normalization.
Guenther U.P., Weinberg D.E., Zubradt M.M., Tedeschi F.A., Stawicki B.N., Zagore L.L., Brar G.A., Licatalosi D.D., Bartel D.P., Weissman J.S, & Jankowsky E. (2018). The helicase Ded1p controls use of near-cognate translation initiation codons in 5′UTRs. Nature, 559(7712), 130-134.
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2

Yeast Ded1p Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysates from yeast strain A14201 at vegetative phase and stage 11 (ndt80 release timecourse) were prepared as described.15 (link) After loading equal amounts of protein on a 10% NEXT gel, denaturing gel electrophoresisand transfer to a PVDF membrane, Western blotting was performedwith anti-Ded1p (Rabbit; 1:5000) and anti-Hexokinase(Rabbit;1:10,000; US Biological) antibodies. Chemiluminescencewas quantified by Imagequant software. Hexokinaseserved as a loading control for normalization.
Guenther U.P., Weinberg D.E., Zubradt M.M., Tedeschi F.A., Stawicki B.N., Zagore L.L., Brar G.A., Licatalosi D.D., Bartel D.P., Weissman J.S, & Jankowsky E. (2018). The helicase Ded1p controls use of near-cognate translation initiation codons in 5′UTRs. Nature, 559(7712), 130-134.
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