Pocketchem ba

Manufactured by Arkray

The PocketChem BA is a portable electrochemical analyzer designed for on-site testing. It provides quantitative analysis of various analytes in a compact and easy-to-use format.

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Lab products found in correlation

Piccolo general chemistry 13 panel, by Abaxis (2 mentions) Cdi 500, by Terumo (1 mentions) Piccolo xpress analyzer, by Abaxis (1 mentions) Fluoroskan ascent, by Thermo Fisher Scientific (1 mentions) Bm6070, by JEOL (1 mentions) Xe 5000, by Sysmex (1 mentions)

4 protocols using pocketchem ba

Multimodal Blood Analysis Protocol

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Blood gas analysis (BGA) from arterial (HA), portal (PV) and caval (VC) lines was performed using the Radiometer ABL90 FLEX. The BGA data was used to calibrate the glucose sensor and the online sensor of the Terumo^®, CDI 500 device. A Piccolo Xpress^® analyser (Abaxis) was used for the quantitative determination of blood urea nitrogen (BUN), albumin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (tBil), gamma glutamyltransferase (GGT) and alkaline phosphatase (ALP) using the Piccolo general chemistry 13 panel (Abaxis). Ammonia levels were determined with the PocketChem BA (PA-4140, Arkray). Factor V was measured by the clinical laboratory of the University Hospital Zurich.

Eshmuminov D., Becker D., Bautista Borrego L., Hefti M., Schuler M.J., Hagedorn C., Muller X., Mueller M., Onder C., Graf R., Weber A., Dutkowski P., Rudolf von Rohr P, & Clavien P.A. (2020). An integrated perfusion machine preserves injured human livers for 1 week. Nature Biotechnology, 38(2), 189-198.

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Comprehensive Liver Function Evaluation

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Blood-gas analysis from arterial (hepatic artery), portal (portal vein) and caval (vena cava) lines was performed using the Radiometer ABL90 FLEX. The blood-gas analysis data were used to calibrate the glucose sensor and the online sensors of the Terumo, CDI 500 device. A Piccolo Xpress analyzer (Abaxis) was used for the quantitative determination of BUN, albumin, alanine aminotransferase (ALT), AST, total bilirubin, gamma glutamyltransferase and alkaline phosphatase using the Piccolo general chemistry 13 panel (Abaxis). Ammonia levels were determined with the PocketChem BA (PA-4140, Arkray). Factor V was measured by the clinical laboratory of the University Hospital Zurich.

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Ammonia Quantification in Blood

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Ammonia content in human blood was measured as described previously [5 (link)]. By duplicate, aliquots of blood (150 μl) were added to 150 μl of ice-cold 10 % trichloroacetic acid. After centrifugation at 12,000×g for 10 min at 4 °C, the supernatant was collected and neutralized with 15 μl of KHCO₃ to pH 7. In duplicates, 50-μl samples were mixed with 32.5 μl of the reaction mixture (phosphate buffer 0.2 M, pH 8; α-ketoglutarate 0.6 M pH 7; NADH 10 mM) and added 10 μl of glutamate dehydrogenase. Ammonia was measured with a microplate fluorimeter (Fluoroskan Ascent, Thermoscientific) using excitation and emission filters of 355 and 460 nm, respectively. Ammonia from PCA rats was measured using the kit II Ammonia Arkray test (PocketChem BA, Arkray) according the kit protocol, using 20 μL of blood.

Tenorio-Laranga J., Montoliu C., Urios A., Hernandez-Rabaza V., Ahabrach H., García-Horsman J.A, & Felipo V. (2015). The expression levels of prolyl oligopeptidase responds not only to neuroinflammation but also to systemic inflammation upon liver failure in rat models and cirrhotic patients. Journal of Neuroinflammation, 12, 183.

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Comprehensive Biochemical Profiling

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The following blood biochemical tests were performed: serum levels of aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LD), alkaline phosphatase (ALP), gamma-glutamyl transpeptidase (GGT), albumin, total bilirubin, blood urea nitrogen (BUN), creatinine, estimated glomerular filtration rate (eGFR), sodium, and potassium, which were measured using a BM-6070 (JEOL Ltd., Tokyo, Japan). Hemoglobin A1c (HbA1c) was measured using HA8181 (ARKRAY Inc. Kyoto, Japan). The prothrombin activity was measured using a CS2100 (Sysmex, Kobe, Japan). Alpha-fetoprotein and des-γ-carboxy prothrombin were measured using the Lumipulse G1200 (Fujirevio, Tokyo, Japan). The platelet count was measured using a Sysmex XE-5000. Ammonia was measured using Pocket Chem BA (ARKRAY Inc).

Narao H., Hirota K., Koya S., Tomita M., Manako Y., Ogawa S., Nakao N., Tsutsumi T., Nakano D., Hashida R., Kawaguchi T., Matsuse H., Nagamatu H, & Torimura T. (2020). Effects of In-Hospital Physical Therapy on Activities of Daily Living in Patients with Hepatocellular Carcinoma. International Journal of Environmental Research and Public Health, 17(23), 9098.

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