Ammonium sulfate nh4 2so4

Manufactured by Merck Group

Sourced in United States, Germany

Ammonium sulfate [(NH4)2SO4] is a crystalline inorganic compound used in various laboratory applications. It functions as a source of ammonium and sulfate ions. Ammonium sulfate is commonly employed in biochemical and analytical procedures, such as protein precipitation and purification.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Ammonium sulfate (234 citations) (NH4)2SO4 (98 citations)

16 protocols using ammonium sulfate nh4 2so4

Camel IgG Purification and Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Protein A sepharose gel has a high binding capacity toward camel immunoglobulin G (IgG). Ammonium sulfate (NH₄)₂SO₄, monobasic sodium phosphate (NaH₂PO₄), dibasic sodium phosphate (Na₂HPO₄), orthophenylenediamine, HRP, bovine serum albumin, and protein A conjugate peroxidase were purchased from Sigma. Sera from camels, immunized goats and rabbits were used as the source of polyclonal camel IgG and anti-camel IgG (Bacterial and parasitic antigens were donated by Prof. Dr. Eman H. Abdel-Rahman).

Abdel-Rahman E.H., El-Jakee J.K., Hatem M.E., Ata N.S, & Fouad E.A. (2017). Preparation of goat and rabbit anti-camel immunoglobulin G whole molecule labeled with horseradish peroxidase. Veterinary World, 10(1), 92-100.

+ Open protocol

+ Expand

Synthesis of Nanocatalyst Materials

Check if the same lab product or an alternative is used in the 5 most similar protocols

Titanium (IV) oxide (P25, Sinopharm Chemical Reagent Co., Ltd, analytically pure), zirconium nitrate pentahydrate (Aladdin, Z190748, 99.5%), cerium nitrate hexahydrate (Aladdin, C105378, 99.5%), sodium hydroxide (Macklin, S817977, ≥ 98%), salicylic acid (Macklin, S817529, 99.5%), potassium sodium tartrate tetrahydrate (Macklin, P816438, 99.5%), sodium nitroferricyanide(III) dehydrate (Macklin, S818341, 99.98% metals basis), sodium hypochlorite solution (Macklin, S828471, available chlorine 4.0%), ammonium chloride (Maclin, A801305, 99.8%), hydrazine monohydrate (Alfa Aesar, A14005, 98%), hydrochloric acid (Sinopharm Chemical Reagent Co., Ltd, 10011018, 36.0−38.0%), potassium hydroxid (Aladdin, P112287, 99.99% metals basis), sulfuric acid (Sinopharm Chemical Reagent Co., Ltd, 10021618, 95.0−98.0%), ethyl alcohol (Sinopharm Chemical Reagent Co., Ltd, 10009218, ≥ 99.7%), 4-(dimethylamino) benzaldehyde (Sigma-Aldrich, 156477, 99%), Nafion solution (Dupont), 211 Nafion membrane (Dupont), deionized (DI) water (Millipore, 18.2 MΩ cm), N₂ gas (99.99%), Ar gas (99.99%). Ammonium sulfate ((NH₄)₂SO₄, 99%), (¹⁵NH₄)₂SO₄, 98 atom% ¹⁵N), and nitrogen-¹⁵N₂ (98 atom% ¹⁵N) were purchased from Sigma-Aldrich. All chemical reagents were used as received without further purification.

Cao N., Chen Z., Zang K., Xu J., Zhong J., Luo J., Xu X, & Zheng G. (2019). Doping strain induced bi-Ti3+ pairs for efficient N2 activation and electrocatalytic fixation. Nature Communications, 10, 2877.

+ Open protocol

+ Expand

Poloxamer 407 and Ammonium Sulfate Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ammonium sulfate ((NH₄)₂SO₄) and poloxamer 407 NF were purchased from Sigma Aldrich. Dichloromethane (DCM) and undenatured ethanol USP were obtained from Spectrum Chemicals. Phosphate Buffered Saline pH 7.2 (PBS 1X) was obtained from Life Technologies. Tris-Buffered Saline pH 7.2 (Tris 1X) was made in the lab.

Fakhari A., Corcoran M, & Schwarz A. (2017). Thermogelling properties of purified poloxamer 407. Heliyon, 3(8), e00390.

+ Open protocol

+ Expand

Synthesis of Hybrid Electrochemical Catalysts

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals were used as received without further purification: potassium permanganate (KMnO₄, QReC), ammonium sulfate ((NH₄)₂SO₄, Sigma-Aldrich), urea (Ajax Finechem), choline chloride (Sigma-Aldrich), zinc chloride (ZnCl₂, Ajax Finechem), zinc sulfate (ZnSO₄, Ajax Finechem), carbon cloth (AvCarb 1071 HCB, AvCarb Material Solutions), deionized water, isopropyl alcohol (IPA, Ajax Finechem) and nickel foam (99.97%, 100 pores per inch (PPI) and 1 mm thick, Qijing Trading Co., Ltd.), sulfuric acid (H₂SO₄, Ajax Finechem).

Corpuz R.D., De Juan L.M., Praserthdam S., Pornprasertsuk R., Yonezawa T., Nguyen M.T, & Kheawhom S. (2019). Annealing induced a well-ordered single crystal δ-MnO2 and its electrochemical performance in zinc-ion battery. Scientific Reports, 9, 15107.

+ Open protocol

+ Expand

Phosphate Solubilization under Salt Stress

Check if the same lab product or an alternative is used in the 5 most similar protocols

The bacteria’s ability to solubilize phosphate was determined using the method of Nautiyal [31 (link)], with modifications that allowed to determine the ability to solubilize phosphate under salt stress. Bacteria were grown in modified Pikosvakaya medium, composed of 10 g of D-glucose, 5 g of tricalcium phosphate (Ca₃(PO₄)₂) (Sigma), 0.5 g of ammonium sulfate ((NH₄)₂SO₄) (Sigma), 0.2 g of NaCl (Sigma), 0.1 g of magnesium sulfate heptahydrate (MgSO₄·7H₂O) (Merck, Rahway, NJ, USA), 0.2 g of potassium chloride (KCl) (Merck), 0.5 g yeast extract (Alfa Aesar, Haverhill, MA, USA), 0.002 g of manganese sulfate monohydrate (MnSO₄·H₂O) (Sigma) and 0.002 g of iron (II) sulfate heptahydrate (FeSO₄·7H₂O) (Merck). The modified medium was sterilized in an autoclave for 20 min, at 120 °C. The bacteria were inoculated, and the plates were incubated for 10 days at 26 °C. After 10 days the colony and halo zone diameter (including the colony) were measured, allowing us to calculate the solubilization index (SI = Halo diameter/Colony diameter).

Cruz C., Cardoso P., Santos J., Matos D., Sá C, & Figueira E. (2023). Application of Plant Growth-Promoting Bacteria from Cape Verde to Increase Maize Tolerance to Salinity. Antioxidants, 12(2), 488.

+ Open protocol

+ Expand

Electrochemical Synthesis of Zinc Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals were used as received without further purification: potassium permanganate (KMnO₄), QRëC, Auckland, New Zealand; ammonium sulfate ((NH₄)₂SO₄), Sigma-Aldrich, St. Louis, MO, USA; carbon cloth (AvCarb 1071 HCB), AvCarb Material Solutions, Lowell, MA, USA; deionized (DI) water; isopropyl alcohol (IPA), Ajax Finechem, Auckland, New Zealand; sulfuric acid (H₂SO₄), Ajax Finechem, Auckland, New Zealand; zinc trifluoromethanesulfonate (Zn(OTf)₂), Sigma-Aldrich, St. Louis, MO, USA; and Dimethyl sulfoxide (DMSO), Sigma-Aldrich, St. Louis, MO, USA.

Corpuz R.D., De Juan-Corpuz L.M., Nguyen M.T., Yonezawa T., Wu H.L., Somwangthanaroj A, & Kheawhom S. (2020). Binder-Free α-MnO2 Nanowires on Carbon Cloth as Cathode Material for Zinc-Ion Batteries. International Journal of Molecular Sciences, 21(9), 3113.

+ Open protocol

+ Expand

Enzymatic Assay for Ammonia Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols

The reaction buffer (TK-buffer) containing 0.5 M triethanolamine and 15 mM α-ketoglutaric acid (both Sigma), pH 8.0 was prepared. This buffer was stored at 4 °C for maximum of 4 weeks.

Fresh NADH stock solution was prepared by dissolving 40 mg NADH (Roche) in 10 ml TK-buffer.

To create a NADH work solution, the NADH stock solution was further diluted (10 times) in TK-buffer. This NADH work solution was stored on ice until further use.

The GIDH stock solution was prepared by adding 3.6 ml MilliQ water to 3000 U glutamate dehydrogenase (Roche). This stock solution was stored at 4 °C.

Prior to the assay, a GIDH work solution was prepared by diluting the GIDH stock solution, 10 times in MilliQ water. The GIDH work solution was stored on ice until further use.

A 4 mM ammonium stock was prepared by diluting a 40 mM ammonium sulfate (NH₄)₂SO₄ (Sigma), 20 times in TK-buffer.

Ammonium standards were prepared in the range of 0–2.8 mM, with 0.4 mM intervals.

Hoogenkamp M.A, & ten Cate J.M. (2014). Determination of arginine catabolism by salivary pellet. MethodsX, 1, 1-5.

+ Open protocol

+ Expand

Lipid Nanoparticle Formulation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hydrogenated soy L-α-phosphatidylcholine (HSPC, >99%), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC, >99%), soy phosphatidylcholine (Soy PC, >99%) and 1,2-distearoyl-sn-glycero-3phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt) (DSPE-PEG2000, >99%) were purchased from Lipoid (Ludwigshafen, Germany). Cholesterol (chol, >99%), doxorubicin hydrochloride (DOX) European Pharmacopoeia Reference Standard, acridine orange (75% dye content), vincristine sulfate (95 -105%), sodium azide (NaN3, >99%), ammonium bicarbonate (NH4HCO3, >99%), 2-(N-morpholino) ethane sulfonic acid (MES, >99%), 5% w/v hydroxypropylcyclodextrin (HP-CD), 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DilC, >98%), histidine >99%, sucrose >99% and ammonium sulfate ([NH4]2SO4, >99%) were obtained from Sigma-Aldrich Ltd. (Poole, UK). Phosphate buffered saline tablets (10 mM, PBS pH 7.3) were acquired from Oxoid Ltd (Basingstoke, UK). All other reagents (ethanol, methanol, isopropyl alcohol) were of analytical grade (HPLC grade, >99.8%) and purchased from commercially available suppliers.
Ultrapure-water was provided by an in-house system.

Roces C.B., Port E.C., Daskalakis N.N., Watts J.A., Aylott J.W., Halbert G.W, & Perrie Y. (2020). Rapid scale-up and production of active-loaded PEGylated liposomes. International journal of pharmaceutics, 586.

+ Open protocol

+ Expand

Analytical-Grade Reagent Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents used [sodium carbonate (Na2CO3), sodium bicarbonate (NaHCO3), sodium dihydrogen phosphate (NaH2PO4) and ammonium sulfate (NH4)2SO4] were obtained from Sigma Chemicals Co. (St. Louis, MO, USA). They were of analytical grade and were used without further purification. The deionized water was employed as solvent throughout the experiments.

Zakanda F.N., Bobanga T., Kabututu P.Z., & Iyamba J.L. (2020). Larvicidal Activity of Inorganic Salts Against Anopheles Stephensi and Culex Quinquefasciatus. International Annals of Science, 10(1), 45-51.

+ Open protocol

+ Expand

Extraction and Purification of Bromelain

Check if the same lab product or an alternative is used in the 5 most similar protocols

All the reagents used for the experiment were research grade. Ammonium sulfate (NH4)2SO4, casein, bovine serum albumin (BSA), L-tyrosine, trichloroacetic acid (CCl3COOH), hydrochloric acid (HCL), ethylene diaminetetraacetic acid (EDTA), betamercaptoethanol (ME), sodium dodecyl sulfate (SDS), Coomassie brilliant blue G250, stem bromelain (lyophilized powder), folin dye, sodium carbonate, sodium potassium tartrate and copper sulphate were purchased from Sigma-Aldrich Co., LLC, USA. Sodium hydroxide (NaOH) was procured from Reidel-de-haen, Seelze, Germany and molecular weight marker was obtained from Molequle on, Auckland, New Zealand.

Gul A., Siddiqui M., Arain H., Khan S., Khan H., & Ishrat U. (2021). Extraction, Partial Purification and Characterization of Bromelain from Pineapple (Ananas Comosus) Crown, Core and Peel Waste. Brazilian Archives of Biology and Technology, 64.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!