Smz1000 dissection microscope

Manufactured by Nikon

Sourced in Japan

The Nikon SMZ1000 is a dissection microscope designed for a variety of laboratory applications. It features a zoom range of 0.75x to 7.5x, providing versatile magnification options. The microscope is equipped with a built-in illumination system and offers a wide field of view, enabling detailed observations of specimens.

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Lab products found in correlation

Dxm1200, by Nikon (5 mentions) Dxm1200 digital camera, by Nikon (3 mentions)

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SMZ1000 (181 citations) SMZ1000 microscope (16 citations) Dissection microscope (16 citations)

12 protocols using smz1000 dissection microscope

Dental Phenotyping of Genetically Engineered Mice

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Two‐week‐old mice were anesthetized with isoflurane for bodyweight assessment and facial photos. Next, they were sacrificed and perfused with 1× phosphate‐buffered saline (PBS) for 10 minutes. Their mandibles were denuded of soft tissues, post‐fixed by immersion in 4% paraformaldehyde (PFA), overnight, and rinsed with PBS three times, for 5 minutes each. The teeth were cleaned with 1% bleach (sodium hypochlorite), rinsed with PBS, air dried, displayed on the Nikon SMZ1000 dissection microscope, and photographed using a Nikon DXM1200 digital camera. Detailed methods for the generation of Relt⁻ mice using CRISPR/Cas9 (Methods S1), the mouse zygote microinjection procedure (Methods S2), and backscattered scanning electron microscopy of molars and incisors (Methods S3) are provided in the Supplemental Data.

Kim J., Zhang H., Seymen F., Koruyucu M., Hu Y., Kang J., Kim Y.J., Ikeda A., Kasimoglu Y., Bayram M., Zhang C., Kawasaki K., Bartlett J.D., Saunders T.L., Simmer J.P, & Hu J.C. (2018). Mutations in RELT cause autosomal recessive amelogenesis imperfecta. Clinical Genetics, 95(3), 375-383.

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Chub Age Estimation from Scale Annuli

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Age estimation based on annuli counts from calcified tissues such as scales has been routinely used for chub (Hellawell 1971a (link); Mann 1976 (link)). Scale-derived growth data allow long-term assessment of the effects of perturbations (i.e. growth pre- and post-crayfish invasion). Chub were sampled by angling in the Rother (n = 32) and Chad Brook (n = 36) during June–September in 2 years: 2008 and 2011. Mass (±1 g) and fork length (±1 mm) were determined in the field and three scales were removed from each chub from the flank between the dorsal fin and lateral line. All individuals were returned alive. For the Rivers Evenlode (n = 68) and Cherwell (n = 58), archived scales provided by the Environment Agency from chub caught before 2000 (Evenlode) and 1995 (Cherwell) were used to obtain pre-invasion growth data, while scales from chub spawned after 2000 and 1995 were used to obtain post-invasion data. Scales were examined using a SMZ1000 dissection microscope (Nikon, Japan) and estimates of length-at-age were back calculated using the Fraser-Lee formula, assuming a length of first scale formation of 15.9 mm (Economou et al. 1991 (link)).

Wood K.A., Hayes R.B., England J, & Grey J. (2016). Invasive crayfish impacts on native fish diet and growth vary with fish life stage. Aquatic Sciences, 79(1), 113-125.

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Whole Mount Staining of Mouse Mammary Glands

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Mammary glands from mice of different age groups as indicated were used for whole mount staining. Inguinal mammary glands were isolated and fixed in Carnoy’s fixative (ethanol: chloroform: glacial acetic acid, 60:30:10) overnight at room temperature. They were rehydrated in descending grades of alcohol (70%, 50%, and 30%) for 15 min each, then rinsed with distilled water before putting in Carmine alum for overnight staining. Stained glands were dehydrated in ascending grades of alcohol (70% twice, 90%, 95%, and 100% twice) for 15 min each, and cleared with Citrisolv reagent (Fisher, Cat#. 22-143975). Glands were mounted and examined under a Nikon SMZ1000 dissection microscope. Eclipse software was used to measure ductal length of calibrated image. Average length of three longest ducts from nipple region was used to represent the ductal length of each animal.

Chiang H.C., Zhang X., Zhao X., Zhang C., Chen J., Garza P., Smith S., Ludwig T., Baer R.J., Li R, & Hu Y. (2018). Gene-Specific Genetic Complementation between Brca1 and Cobra1 During Mouse Mammary Gland Development. Scientific Reports, 8, 2731.

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Craniofacial Phenotyping of Acp4 Mutant Mice

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Frontal facial images were taken of 7-week Acp4^+/+, Acp4^+/R110C, and Acp4^R110C/R110C mice lightly anesthetized with isoflurane using the Nikon SMZ1000 dissection microscope with a Nikon DXM1200 digital camera. Three samples from each genotype were evaluated. Separately, another group of mice were deeply anesthetized with isoflurane and perfused with 1× phosphate buffered saline (PBS) for 10 min. Mandibles were dissected out and cleaned of soft tissues, post-fixed by immersion in 4% paraformaldehyde (PFA) overnight, then rinsed with PBS three times for 5 min each. Hemimandibles were cleaned with 1% bleach (sodium hypochlorite), rinsed with PBS, air dried, displayed on the dissection microscope, and photographed, as described previously^{50 (link)}.

Liang T., Wang S.K., Smith C., Zhang H., Hu Y., Seymen F., Koruyucu M., Kasimoglu Y., Kim J.W., Zhang C., Saunders T.L., Simmer J.P, & Hu J.C. (2022). Enamel defects in Acp4R110C/R110C mice and human ACP4 mutations. Scientific Reports, 12, 16477.

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Mandibular Tissue Fixation and Tooth Imaging

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Seven‐week‐old mice were anesthetized with isoflurane, sacrificed, and perfused with 4% paraformaldehyde (PFA) for 10 m. Their mandibles were denuded of soft tissues, post‐fixed by immersion in 4% PFA overnight, and rinsed with phosphate‐buffered saline (PBS) three times, for 5 min each. The teeth were cleaned with 1% bleach (sodium hypochlorite), rinsed with PBS, air dried, displayed on the Nikon SMZ1000 dissection microscope, and photographed using a Nikon DXM1200 digital camera.

Hu Y., Smith C.E., Cai Z., Donnelly L.A., Yang J., Hu J.C, & Simmer J.P. (2016). Enamel ribbons, surface nodules, and octacalcium phosphate in C57BL/6 Amelx −/− mice and Amelx +/− lyonization. Molecular Genetics & Genomic Medicine, 4(6), 641-661.

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Phenotypic Characterization of Fam83h Mutant Mice

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Heterozygous mutant (Fam83h^+/Tr) mice were mated to generate three mouse Fam83h genotypes (+/+, +/Tr, and Tr/Tr), which were then evaluated for their physical appearance, activity, growth rate, size difference, food intake, and fertility. The homozygous (Fam83h^Tr/Tr) mice were viable and fertile. For gross evaluation of the dental enamel, mice were put under anesthesia using isofluorane, and their incisors were inspected under a dissection microscope. Seven‐week‐old mice were sacrificed and fixative‐perfused with 4% paraformaldehyde (PFA). The mandibles were removed and sliced through the mental symphysis with a razor blade to generate hemimandibles. These were carefully dissected free of soft tissues under a stereoscopic microscope using tissue forceps and a spoon excavator. The hemimandibles were submerged in 1% NaClO for 5 min, rinsed, air dried, and photographed using a Nikon SMZ1000 dissection microscope equipped with a Nikon digital camera DXM1200 (Melville, NY).

Wang S., Hu Y., Smith C.E., Yang J., Zeng C., Kim J., Hu J.C, & Simmer J.P. (2019). The Enamel Phenotype in Homozygous Fam83h Truncation Mice. Molecular Genetics & Genomic Medicine, 7(6), e724.

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Ambn Genotype Analysis in Mice

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Ambn^+/+, Ambn^+/lacZ, and Ambn^lacZ/lacZ mice at 7 weeks were anesthetized with isoflurane for facial photos, sacrificed, and perfused with PBS for 10 min. Their mandibles were denuded of soft tissues, postfixed by immersion in 4% PFA overnight, and rinsed with PBS three times, for 5 min each. The teeth were cleaned with 1% bleach (sodium hypochlorite), rinsed with PBS, air dried, displayed on the Nikon SMZ1000 dissection microscope, and photographed using a Nikon DXM1200 digital camera as described previously (Kim et al., 2019).

Liang T., Hu Y., Smith C.E., Richardson A.S., Zhang H., Yang J., Lin B., Wang S., Kim J., Chun Y., Simmer J.P, & Hu J.C. (2019). AMBN mutations causing hypoplastic amelogenesis imperfecta and Ambn knockout‐NLS‐lacZ knockin mice exhibiting failed amelogenesis and Ambn tissue‐specificity. Molecular Genetics & Genomic Medicine, 7(9), e929.

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Characterization of Fam83h Knockout Mice

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Heterozygous mutant (Fam83h^+/−) mice were mated to generate three mouse Fam83h genotypes (+/+, +/−, and −/−), which were then evaluated for their physical appearance, activity, growth rate, size difference, food intake, and fertility. Although the pups of three genotypes were born at the expected Mendelian ratio, almost all the Fam83h^−/− mice died within 2 weeks after birth with only a few surviving to 7 weeks. For gross evaluation of the dental enamel, mice were put under anesthesia using isofluorane, and the incisors were inspected under a dissection microscope. 7‐week‐old mice were sacrificed and fixative‐perfused with 4% paraformaldehyde (PFA) or fixative‐perfused with 2.5% glutaraldehyde. The mandibles were removed and sliced through the mental symphysis with a razor blade to generate hemimandibles. These were carefully dissected free of soft tissues under a stereoscopic microscope using tissue forceps and a spoon excavator. The hemimandibles were submerged in 1% NaClO for 5 min, rinsed, air dried, and photographed using a Nikon SMZ1000 dissection microscope equipped with a Nikon digital camera DXM1200 (Melville, NY).

Wang S., Hu Y., Yang J., Smith C.E., Richardson A.S., Yamakoshi Y., Lee Y., Seymen F., Koruyucu M., Gencay K., Lee M., Choi M., Kim J., Hu J.C, & Simmer J.P. (2015). Fam83h null mice support a neomorphic mechanism for human ADHCAI. Molecular Genetics & Genomic Medicine, 4(1), 46-67.

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Dental Specimen Preparation and Imaging

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Day 14, Day 17, and 9‐week‐old (adult) mice were anesthetized with isoflurane, sacrificed by head dislocation, and fixed by immersion in 4% paraformaldehyde (PFA). The mandibles were removed and dissected free of soft tissues. The teeth were cleaned with nonwoven gauze, displayed on the Nikon SMZ1000 dissection microscope and photographed using a Nikon digital camera DXM1200.

Hu Y., Smith C.E., Richardson A.S., Bartlett J.D., Hu J.C, & Simmer J.P. (2015). MMP20, KLK4, and MMP20/KLK4 double null mice define roles for matrix proteases during dental enamel formation. Molecular Genetics & Genomic Medicine, 4(2), 178-196.

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Dental Enamel Evaluation in Wdr72 Mutant Mice

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Heterozygous mutant (Wdr72^+/−) mice were mated to generate three mouse Wdr72 genotypes (+/+, +/−, and −/−), which were then evaluated for their physical appearance, activity, growth rate, size difference, food intake, and fertility. For gross evaluation of the dental enamel, mice were put under anesthesia using isofluorane, and the incisors were inspected under a dissection microscope. Day 14 and 7-week-old mice were sacrificed and fixative-perfused with 4% paraformaldehyde (PFA). The mandibles were removed and sliced through the mental symphysis with a razor blade to generate hemimandibles. These were carefully dissected free of soft tissues under a stereoscopic microscope using tissue forceps and a spoon excavator. The hemimandibles were submerged in 1% NaClO for 5 min, rinsed, air dried, and photographed using a Nikon SMZ1000 dissection microscope equipped with a Nikon digital camera DXM1200 (Melville, NY).

Wang S.K., Hu Y., Yang J., Smith C.E., Nunez S.M., Richardson A.S., Pal S., Samann A.C., Hu J.C, & Simmer J.P. (2015). Critical roles for WDR72 in calcium transport and matrix protein removal during enamel maturation. Molecular Genetics & Genomic Medicine, 3(4), 302-319.

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