Evolution 60 uv visible spectrophotometer

All animals were sacrificed at a commercial slaughterhouse according to protocols approved by the Institutional Animal Care and Use Committee at the Institute of Animal Science, Chinese Academy of Agricultural Sciences (Approval number: PJ2011-012-03). Longissimus dorsi (LD) muscle samples were collected from Landrace fetuses on the following days post-coitum (dpc): 33, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100, and 105 dpc. LD muscle samples were collected from piglets on the following days after birth (dab): 0, 10, 20, 30, 40, 60, 80, 100, 140, 160, and 180 dab. Three biological replicates were collected at each time point, and totally 78 samples were collected. All samples were immediately frozen in liquid nitrogen and stored at –80 °C until further processing. Total RNA was extracted using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions. RNA quantity and quality was determined by the Evolution 60 UV-Visible Spectrophotometer (Thermo Scientific). RNA preparations with an A₂₆₀/A₂₈₀ ratio of 1.8–2.1 and an A₂₆₀/A₂₃₀ ratio > 2.0 were selected for this assay. RNA integrity was determined by analyzing the 28S/18S ribosomal RNA ratio on 1.5% agar gels. Only RNA preparations that resolved with three clear bands on these gels were used for the transcriptome sequencing and qPCR analysis.

All chemicals and reagents purchased from sigma Aldrich and Daejung chemicals South Korea, and TCI chemicals, Japan without further purifications. Absorption spectra and drug release study were recorded in 1 cm quartz cuvettes using Evolution™ 60 UV–visible Spectrophotometer (Thermo Fischer Scientific, USA). Emission spectra were obtained on a Jasco-FP 6500 spectrofluorometer. Particle size was analyzed by Dynamic Light Scattering Spectrophotometer (Otsuka Electronics Co., Ltd, Japan). Zeta potential was measured by electrophoretic light scattering (ELS) spectrometer (Otsuka Electronics Co., Ltd, Japan). The morphology of the Ru-1@TPP-PEG-Biotin SAN was analyzed using Energy-Filtering Transmission Electron Microscope (EF-TEM, Carl Zeiss, LIBRA 120, Germany). Fourier transform infrared (FTIR) spectroscopy experiments performed using a JASCO, FT/IR-4200 instrument. The human breast cancer cell line MCF-7 and hepatoma cell line HepG2 were supplied from the Korean Cell Line Bank (KCLB, Korea). Quantum dot conjugation kit (Invitrogen, USA) was used to conjugate the antibody with a quantum dot.