H3 ab1791

Rabbit polyclonal antibodies were raised against H4R3me2a and N‐acH4 by Eurogentec (Belgium). Additional details and characterization of these antibodies are provided in 46, 47. Other antibodies used were as follows: H4 (62‐141‐13; Millipore), H3 (ab1791; Abcam), Naa40⁵¹ (a gift from Qwei Zhai), and IgG (NB810‐56910; Novus Biologicals).

Atorvastatin (344423) and 2,3,5-triphenyltetrazolium chloride monohydrate (TTC) (T8877) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Atorvastatin was dissolved with DMSO and then diluted with ultrapure water to the desired concentration. The final concentration of DMSO in the atorvastatin solution administered to the animals is 0.5%. The total RNA extraction kit (LS1040) was purchased from Promega Biotechnology Co., Ltd. (Beijing, China). SYBR (Q711) was purchased from Vazyme Biotech Co., Ltd. (Nanjing, China). Recombinant mouse sclerostin (SOST) protein (1589-ST), which can inhibit Wnt/β-catenin signaling pathway by antagonizing the binding of Wnt to its receptor and reducing the expression of downstream β-catenin [41 (link), 42 (link)], was purchased from R&D Systems (Minnesota, MN, USA). XAV939 (S1180), which can inhibit Wnt/β-catenin signaling pathway by degrading β-catenin, was purchased from American Selleck Biotechnology Co., Ltd. (China Branch, Shanghai). β-actin (20068) was purchased from Zen Bioscience Co., Ltd. (Chengdu, China). H3 (ab1791) and β-catenin (ab32572) were purchased from Abcam (Cambridge, UK). GSK3β (12456) and p-GSK3β (9322) were purchased from CST (Boston, USA).

Chemicals and reagents were obtained from Sigma (St. Louis, MO, USA), RPI (IL, USA) or Fisher Scientific (Pittsburgh, PA, USA). We used the following antibodies in this study: purified monoclonal Npm antibody [39 (link)], B4, H1A, Nap1 [40 (link),41 (link)]. Anti- β-tubulin antibody (clone E7) is from the Developmental Studies Hybridoma Bank at the University of Iowa. The following antibodies were from Millipore: H2A (07–146), H2A.Z (07–594), H4 (07–108), and H2A/H4S1ph (07–179). The following antibodies were from Abcam: H3 (ab1791) and H4R3me2s (ab5823). H2A/H4R3me1 and H2A/H4R3me2s antibodies were used as described [42 (link)].

All antibodies used in the experiments were purchased. Actin (AF5003, 1:1000) and LMNB1 (AF1408, 1:1000) were purchased from Beyotime; P21 (10355-1-AP, 1:1000) was purchased from Proteintech (Wuhan, China); PHGDH (66350, 1:1000), yH2AX (9718, 1:800) and Ki67 (9129, 1:400) were purchased from Cell Signaling Technology (Danvers, MA, USA); PSAT1 (53804, 1:1000) was purchased from Signalway Antibody (Greenbelt, MD, USA); PSPH (DF12711, 1:1000) was purchased from Affinity Biosciences (Cincinnati, OH, USA); H3 (ab1791, 1:1000), H3K4me3 (ab213224, 1:1000), H3K9me3 (ab176916, 1:1000), H3K27me3 (ab192985, 1:1000), H3K36me3 (ab282572, 1:1000) were purchased from Abcam (Waltham, MA, USA).