Milk yield was recorded at every milking in the milking parlor (rotary milking parlor, milk meter MM25, DeLaval France, Elancourt, France). Milk fat, protein, and lactose contents were determined from milk samples collected at each milking, 2 or 3 d a week during cTDM, pTDM1, pTDM2, and pTDM3 and on the 24h-MI day. Somatic cell count was measured once a day on the same days. Milk fat, protein, and lactose contents were determined by infrared analysis (Milkoscan, Foss Electric, Hillerød, Denmark), and SCC with a Fossomatic cell counter (Foss Electric).
Milkoscan
Manufactured by Foss
Sourced in Denmark
The MilkoScan is a laboratory instrument designed for the analysis of milk and dairy products. It provides accurate and reliable measurements of various milk components, such as fat, protein, lactose, and solids, using infrared spectroscopy technology.
Automatically generated - may contain errors
Lab products found in correlation
Fossomatic, by Foss (5 mentions)
Fossomatic cell counter, by Foss (1 mentions)
Infinium ovine snp50 v1 beadchip, by Illumina (1 mentions)
Milkoscan 6000, by Foss (1 mentions)
Foodscan, by Foss (1 mentions)
Fossomatic 7dc, by Foss (1 mentions)
Pan mouse igg, by Thermo Fisher Scientific (1 mentions)
Technicon ra 1000, by Bayer (1 mentions)
35 protocols using milkoscan
1
Milk Quality Monitoring Protocol
2
Quantitative Analysis of Cannabinoids in Cow Milk
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The milk yield of each cow was recorded for 3 consecutive days (d18, 19, and 20) during the experimental period. Milk samples were collected from each cow while the cows were milked in a parallel milking parlor. According to the real milk yield of cows three times a day, milk samples (50 mL) were mixed with potassium dichromate and stored at 4°C until used for subsequent determination of milk components. The protein, fat, lactose, and milk urea nitrogen (MUN) concentrations and SCC of milk samples were analyzed by a 4-channel spectrophotometer (MilkoScan; Foss Electric, Hillerød, Denmark) at the Heilongjiang Academy of Agricultural Reclamation (Harbin, China). CBD and THC in milk were analyzed by liquid chromatography-mass spectrometry (Triple Quad 5500 + QTRAP Ready; AB Sciex Ltd., USA), according to the methods of Escrivá et al. (27 (link)). The LOD and LOQ of cannabinoids in milk were 1.5 ng/ml and 5 ng/ml.
3
Dairy Goat Milk Yield and Composition
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Goats were milked twice a day at 8:00 am and at 18:00 pm and milk yield were recorded daily. The milk sample was taken to assay the milk fat and milk protein concentrations using MilkoScan (FT1, Foss, Hillerod, Denmark).
4
Milk Yield and Composition Analysis
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Individual milk yield was measured twice a day at 04:00 and 15:00. On the third day of the occupation period, an individual milk sample was collected in both the morning and afternoon milking to determine milk fat, protein, and lactose concentration by mid infrared spectrophotometry (Milko-Scan, Foss Electric, Hillerød, Denmark).
5
Milk Composition and Somatic Cell Count in Sarda Ewes
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A total of 2,358 test-day records measured on 509 pluriparous ewes (3-7 records per animal, with an average value, ± SD, of 5.2 ± 1.1) farmed in 32 flocks located in Sardinia (Italy) was available. Animals were sampled during only one lactation, in the period from January to July 2015. Milk composition (fat, protein, lactose, NaCl, and urea contents) was determined by Fourier transform mid-infrared spectroscopy using a MilkoScan 6000 instrument (MilkoScan, Foss Electric). The SCC was determined using the Fossomatic (Foss Electric).
All animals were officially recorded in the Sarda Herd Book. A complete pedigree was retrieved. All ewes were genotyped with the Illumina Infinium Ovine SNP50 v1 BeadChip, for 45,813 markers (after quality control). Markers were mapped on the OAR 4.0 release. Details about the genotyping procedure are reported by Cesarani et al. (2019) (link).
All animals were officially recorded in the Sarda Herd Book. A complete pedigree was retrieved. All ewes were genotyped with the Illumina Infinium Ovine SNP50 v1 BeadChip, for 45,813 markers (after quality control). Markers were mapped on the OAR 4.0 release. Details about the genotyping procedure are reported by Cesarani et al. (2019) (link).
6
Dairy Cow Milk Composition Analysis
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Cows were milked at 0600 and 1530 h in a tandem milking parlor, and the milk yield of each cow was recorded daily. Milk samples were collected at the morning and afternoon milking from d 34 to 37 postpartum and analyzed for fat, protein, lactose, ash, MUN, and SCC using MilkoScan (Foss Electric, Hillerød, Denmark).
7
Milk Composition Analysis in Dairy Cows
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The experiment lasted for 8 weeks, following a week for the cows to adapt to the diets. Milk sampling devices (Waikato Milking Systems NZ Ltd., Hamilton, New Zealand) were attached to the milking machines to measure milk weight and collect samples. Milk production was recorded for all three milking times. A 50-mL aliquot of milk was collected weekly at each milking, proportional to yield (4:3:3, composite). The composited milk sample, with added Bronopol tablets (milk preservative, D & F Control Systems, San Ramon, CA, USA), was stored at 4°C for later analysis of fat, protein and lactose by infrared analysis (Laporte and Paquin, 1999 (link)) using a four-channel spectrophotometer (Milk-o-Scan, Foss Electric A/S, Hillerød, Denmark).
8
Milk Composition and Quality in Sarda Ewes
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The study was carried out on 991 Sarda ewes in 47 flocks (about 18 ewes per herd) located in the 4 historical provinces of Sardinia, Italy. All the ewes involved in the experiment were officially recorded in the herd book of Sarda breed. The individual milk samples (1 per animal from the morning milking) were collected between April and July 2014. Preservative was added to all milk samples, stored at room temperature, and processed 24 h after collection. Chemical composition of milk (fat percentage, protein percentage, lactose percentage, pH, urea, NaCl) and cryoscopy index were determined by Fourier transform mid-infrared (FT-MIR) spectroscopy equipment (MilkoScan, Foss Electric, Hillerød, Denmark) . Calibration algorithms were developed according to FIL-IDF rules (ISO 9622:2013; IDF, 2013) . Somatic cell count values (Fossomatic, Foss Electric) were also measured and converted to SCS by logarithm transformation as proposed by Ali and Shook (1980) .
9
Monthly Milk Lactose Analysis
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Milk samples from 1481 animals (50 ml each) were monthly collected. The samples were maintained frozen at -20• C until performed the chemical analysis of milk constituents. Percentage of lactose was determined by Infrared Milk Analyzer (Bentley, I50®) and the automated method of infrared absorption spectrophotometry (Milk-o-Scan; Foss Electric, Hillerφd, Denmark) at Cattle Information System/Egypt (CISE) and Animal Production Research Institute (APRI) of Agricultural Research Center (ARC). Records for chemical composition of milk were checked carefully to remove abnormal phenotypic values and exclude animals that have less than three times chemical analyses per parity. After quality check, a total number of 60,318 monthly measures were remained. The average lactose percentage was 5.1±0.6. Yield of lactose was calculated by multiplying lactose percentage by milk yield at the same day. The average lactose yield was 0.44±0.15 kg/day.
10
Impacts of R. sphaeroides on Milk Production
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Six Holstein lactating cows (body weight 612±27 kg, milk yield 28.4±2.3 kg/d, and parity 2nd) equipped with permanent ruminal cannulae were used in the in vivo experiment, to examine if supplementing diets with R. sphaeroides cultures affected milk production and milk composition, particularly the concentration of CoQ10 in milk. Cows were randomly allocated to one of the two dietary treatments (n = 3): control TMR (C-TMR), identical to that used in the in vitro study, and TMR supplemented (S-TMR) with 0.5% of R. sphaeroides culture (TMR dry matter basis, v/w). Cows were allowed to adapt to the experimental diets for 20 days and at the end of the adaptation to the experimental diets an aliquot of milk samples (30 mL) were then collected at the same time, over three consecutive days and pooled per cow, immediately stored at −20°C, and freeze-dried prior to the CoQ10 analysis. Milk composition was analyzed using 50 mL of milk and Milko-Scan (FOSS-4000, FOSS, Denmark).
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