Anti syntaxin6

Manufactured by Cell Signaling Technology

Anti-syntaxin6 is a laboratory reagent used for the detection and study of the syntaxin6 protein in biological samples. Syntaxin6 is a member of the SNARE protein family and plays a role in the regulation of vesicle trafficking and membrane fusion within cells.

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Lab products found in correlation

Anti cd8 apc cy7, by BD (1 mentions) Anti cd56 pe cf594, by BD (1 mentions) Anti rab5, by Cell Signaling Technology (1 mentions) Anti rab8, by Cell Signaling Technology (1 mentions) Anti rab27a, by Abcam (1 mentions) Anti vamp3, by Abcam (1 mentions) Anti snap23, by Abcam (1 mentions) Chicken anti goat af647, by Thermo Fisher Scientific (1 mentions) Anti rab7, by Santa Cruz Biotechnology (1 mentions) Anti granzyme b, by BD (1 mentions) Anti rab11a, by Cell Signaling Technology (1 mentions) Anti rab37, by Abcam (1 mentions) Goat anti rabbit af647, by Thermo Fisher Scientific (1 mentions) Anti cd71, by Cell Signaling Technology (1 mentions) Dithiothreitol (dtt), by Sinopharm (1 mentions) Anti flag m2 f3165, by Merck Group (1 mentions) Anti calreticulin, by Cell Signaling Technology (1 mentions) Anti gfp ht801 01, by Transgene (1 mentions) Anti cox 4, by Cell Signaling Technology (1 mentions) Anti abcd3, by Merck Group (1 mentions)

3 protocols using anti syntaxin6

Imaging Flow Cytometry for Immune Cell Analysis

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For imaging flow cytometry experiments: anti-CD8 APC-Cy7 (BD Biosciences, San Jose, CA or Tonbo Biosciences, San Diego, CA) and anti-CD56 PE-CF594 (BD Biosciences) were used for surface staining; all intracellular stains included anti-perforin D48 PE or BV421 (Biolegend, San Diego, CA) and anti-perforin δG9 FITC (Biolegend) and one of the following unconjugated antibodies: anti-CD71 (Cell Signaling Technology, Danvers, MA), anti-granzyme B (BD Biosciences), anti-rab3D (Abcam, Cambridge, MA), anti-rab4 (Pierce Antibodies, Waltham, MA), anti-rab5 (Cell Signaling Technology), anti-rab7 (Santa Cruz Biotechnology, Dallas, TX), anti-rab8 (Cell Signaling Technology), anti-rab11a (Cell Signaling Technology), anti-rab27a (Abcam), anti-rab35 (Abcam), anti-rab37 (Abcam), anti-syntaxin6 (Cell Signaling Technology), anti-syntaxin7 (R&D systems, Minneapolis, MN), anti-vti1b (Abcam), anti-VAMP3 (Abcam), anti-VAMP4 (Abcam), or anti-SNAP23 (Abcam). The unconjugated antibodies were detected using goat anti-rabbit AF647, chicken anti-goat AF647, or donkey anti-sheep AF647 secondary antibodies (Invitrogen, Carlsbad, CA). For confocal microscopy, perforin D48 FITC (Abcam), perforin δG9 AF647 (Biolegend), and goat anti-rabbit or donkey anti-sheep AF568 secondary antibodies (Invitrogen) were used, along with the selected rab or SNARE antibody.

Lesteberg K.E., Orange J.S, & Makedonas G. (2017). Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunologic research, 65(5), 1031-1045.

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Characterization of CD59 and DAF in HAP1 cells

Cited 2 times

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HAP1 cells were a gift from T.R. Brummelkamp (Netherlands Cancer Institute, Amsterdam, Netherlands; Carette et al., 2011 (link)). They were cultured in Iscove’s modified Dulbecco’s medium containing 10% FCS with antibiotics needed for selection. HEK293 and its derivative HEK293FF6 cells (Hirata et al., 2015 (link)) were cultured in DMEM containing 10% FCS.
Mouse monoclonal anti-CD59 (clone 5H8; Maeda et al., 2007 (link)), anti-DAF (clone IA10; Maeda et al., 2007 (link)), anti-Flag (F3165; M2; Sigma-Aldrich), anti-GFP (HT801-01; TransGen Biotech), anti-HA (H3663; HA-7; Sigma-Aldrich), anti-ERp57 (K0135-3; MBL), rabbit monoclonal antisyntaxin 6 (2869S; Cell Signaling Technology), anticalreticulin (12238S; Cell Signaling Technology), anti-myc (AM933; Beyotime), and polyclonal anticalnexin (C4731; Sigma-Aldrich) were used as primary antibodies. Phycoerythrin (PE)-conjugated goat anti–mouse IgG (12-4010-87; Thermo Fisher Scientific), HRP-conjugated anti–mouse IgG (HS211-01; TransGen Biotech), and anti–rabbit IgG (HS101-01; TransGen Biotech) were used as the secondary antibodies. DNJ (Cayman Chemical), DMJ (Cayman Chemical), KIF (Cayman Chemical), TG (Sigma-Aldrich), dithiothreitol (Sinopharm), and TM (Sigma-Aldrich) were used for drug treatments.

Liu Y.S., Guo X.Y., Hirata T., Rong Y., Motooka D., Kitajima T., Murakami Y., Gao X.D., Nakamura S., Kinoshita T, & Fujita M. (2018). N-Glycan–dependent protein folding and endoplasmic reticulum retention regulate GPI-anchor processing. The Journal of Cell Biology, 217(2), 585-599.

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Comprehensive Antibody Validation Protocol

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The following antibodies were used: LACC1 E-12 (anti-FAMIN; sc-376231), E-7 (sc-374553), H-6 (sc-376064) from Santa Cruz; anti-β actin (ab6276) and anti-PMP70 (ab3421) from Abcam; anti-β-tubulin (2128), anti-calnexin (2679), anti-calreticulin (12238), anti-catalase (12980), anti-caveolin-2 (8522), anti-CENP-A (2186), anti-COX-IV (4850), anti-EEA1 (3288), anti-fibrillarin (2639), anti-histoneH3 (4499), anti-LAMP1 (9091), anti-LC3B (2868), anti-NUP98 (2598), anti-PDI (2446), anti-Rab5 (3547) and anti-syntaxin-6 (2869) from Cell Signaling; anti-ABCD3 (HPA032027) from Sigma Aldrich.

Assadi G., Vesterlund L., Bonfiglio F., Mazzurana L., Cordeddu L., Schepis D., Mjösberg J., Ruhrmann S., Fabbri A., Vukojevic V., Percipalle P., Salomons F.A., Laurencikiene J., Törkvist L., Halfvarson J, & D’Amato M. (2016). Functional Analyses of the Crohn’s Disease Risk Gene LACC1. PLoS ONE, 11(12), e0168276.

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