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Lincode non targeting control sirnas

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Lincode® non-targeting control siRNAs are a set of small interfering RNA (siRNA) molecules designed to have no known targets in the human, mouse or rat genomes. These control siRNAs are intended to be used as negative controls in RNA interference (RNAi) experiments to help distinguish specific target gene effects from off-target effects.

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Lab products found in correlation

Lincode smartpool sirna targeting, by Horizon Discovery (2 mentions) Lincode sirna library, by Horizon Discovery (2 mentions) Dot1l, by Horizon Discovery (2 mentions)

Spelling variants of this product

Control siRNA (209 citations) SiRNAs (161 citations) Non-targeting control siRNA (92 citations) Non-targeting siRNA (74 citations) SiRNA targeting (10 citations)

3 protocols using lincode non targeting control sirnas

1

Kcnq1ot1 Regulation of Myogenic Differentiation

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8 × 105 C2.7 muscle cells were transfected using a mixture of four chemically synthesized siRNAs duplexes (Kcnq1ot1 MOUSE SMARTpool Dharmacon) and with a non-targeting pool (Lincode Non-targeting Control siRNAs Dharmacon) as negative control, at a final concentration of 100 nM. Kcnq1ot1 expression levels were analyzed 24 h after transfection in proliferation conditions, while 24 h after the shift to differentiation conditions (DMEM containing 0.5% FCS) samples were collected to analyze p57 and p21 expression levels.
Polymorphic fibroblasts (C57BL/6 × SD7) expressing MyoD were transfected 48 h after infection, with 200 nM siRNAs. Samples were collected 24 h after transfection to check the reduction of Kcnq1ot1 expression and 24 h after the shift to differentiation medium to determine p57 and p21 expression levels.
Andresini O., Rossi M.N., Matteini F., Petrai S., Santini T, & Maione R. (2019). The long non-coding RNA Kcnq1ot1 controls maternal p57 expression in muscle cells by promoting H3K27me3 accumulation to an intragenic MyoD-binding region. Epigenetics & Chromatin, 12, 8.
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2

Knockdown of MAYA and Other Targets

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Lincode siRNA library (G-301005-01), Lincode® SMARTpool® siRNA targeting MAYA (R-036866), Lincode® non-targeting control siRNAs (D-001320), and ON-TARGETplus SMARTpool® siRNA targeting ROR1 (L-003171), BCAR3 (L-011469), LLGL2 (L-019812), HER3 (L-003127), EGFR (L-003114), NSUN6 (L-018822), and DOT1L (L-014900) from GE Healthcare Dharmacon were used in this study. The knockdown efficiency and specificity of all siRNAs were validated either by RT-qPCR or immunoblotting. The shRNAs targeting MAYA were designed using the shERWOOD algorithm and cloned into UltramiR scaffold in pZIP lentiviral vector (transOMIC technologies). For in vivo inducible knockdown of MAYA in xenograft experiments, shRNA sequences were constructed into pZIP-TRE3GS lentiviral vector (transOMIC technologies). LNAs targeting MAYA or a scrambled sequence were designed and synthesized from Exiqon. Supplementary Table 7 contains detailed sequence information of siRNAs, shRNAs and LNAs.
Li C., Wang S., Xing Z., Lin A., Liang K., Song J., Hu Q., Yao J., Chen Z., Park P.K., Hawke D.H., Zhou J., Zhou Y., Zhang S., Liang H., Hung M.C., Gallick G.E., Han L., Lin C, & Yang L. (2017). A ROR1-HER3-LncRNA signaling axis modulates the Hippo-YAP pathway to regulate bone metastasis. Nature cell biology, 19(2), 106-119.
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3

Knockdown of MAYA and Other Targets

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Lincode siRNA library (G-301005-01), Lincode® SMARTpool® siRNA targeting MAYA (R-036866), Lincode® non-targeting control siRNAs (D-001320), and ON-TARGETplus SMARTpool® siRNA targeting ROR1 (L-003171), BCAR3 (L-011469), LLGL2 (L-019812), HER3 (L-003127), EGFR (L-003114), NSUN6 (L-018822), and DOT1L (L-014900) from GE Healthcare Dharmacon were used in this study. The knockdown efficiency and specificity of all siRNAs were validated either by RT-qPCR or immunoblotting. The shRNAs targeting MAYA were designed using the shERWOOD algorithm and cloned into UltramiR scaffold in pZIP lentiviral vector (transOMIC technologies). For in vivo inducible knockdown of MAYA in xenograft experiments, shRNA sequences were constructed into pZIP-TRE3GS lentiviral vector (transOMIC technologies). LNAs targeting MAYA or a scrambled sequence were designed and synthesized from Exiqon. Supplementary Table 7 contains detailed sequence information of siRNAs, shRNAs and LNAs.
Li C., Wang S., Xing Z., Lin A., Liang K., Song J., Hu Q., Yao J., Chen Z., Park P.K., Hawke D.H., Zhou J., Zhou Y., Zhang S., Liang H., Hung M.C., Gallick G.E., Han L., Lin C, & Yang L. (2017). A ROR1-HER3-LncRNA signaling axis modulates the Hippo-YAP pathway to regulate bone metastasis. Nature cell biology, 19(2), 106-119.
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