Cytoplasmic-nuclear translocation of NF-κB p65 was analyzed by confocal microscopy according to the method described previously [22 (link)]. Briefly, RAW 264.7 macrophages were pretreated with or without 4'-HW (15 μM) for 1 h and then treated with LPS (100 ng/ml) for another 30 minutes. Treated cells were fixed and incubated with anti-NF-κB p65 primary antibody, followed by incubation with Alex Fluor®488 donkey anti-rabbit antibody (1:500, Invitrogen) and 5 μg/ml of DAPI, and the fluorescent confocal images were captured using a laser-scanning confocal fluorescence microscope (TCS SP8, Leica Microsystems, Wetzlar, Germany).
Alex fluor 488 donkey anti rabbit antibody
Manufactured by Thermo Fisher Scientific
Sourced in Germany, United States
The Alex Fluor®488 donkey anti-rabbit antibody is a secondary antibody conjugated with the Alex Fluor®488 fluorescent dye. It is designed to bind to rabbit primary antibodies, allowing for the detection and visualization of target proteins or other biomolecules in various experimental techniques.
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Lab products found in correlation
2 protocols using alex fluor 488 donkey anti rabbit antibody
1
Visualizing NF-κB Nuclear Translocation
2
Hypoxia-Induced Angiogenesis Regulation
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The agents used in the present study were apatinib (HengRui Medicine Co. LTD, Jiangsu, China), sunitinib (G1430008, Aladdin, Shanghai, China), antibodies against PECAM1 (CD31) (ab182981, Abcam plc), HypoxyprobeTM-1 Plus Kit (Hypoxyprobe, Inc, USA), Glucagon (sc-13091, Santa Cruz Biotechnology, Inc), Insulin (I2018, Sigma-Aldrich), Pcsk1 (ab5543, Abcam plc), Pcsk2 (ab3533, Abcam plc); Alex Fluor 555 donkey anti-mouse antibody and Alex Fluor 488 donkey anti-rabbit antibody (Invitrogen; Thermo Fisher Scientific, Inc.). All the antibodies were tested previously to ensure the validation for cross-species. CCK-8 kit (Dojindo Laboratories, Kumamoto, Japan) was used in our study.
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