Polymerase 2

Manufactured by Santa Cruz Biotechnology

Polymerase II is a key enzyme involved in the transcription of genetic information from DNA to RNA. It is responsible for the synthesis of messenger RNA (mRNA) and other types of RNA molecules, which serve as the templates for protein production within cells. Polymerase II plays a central role in gene expression and cellular function.

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Lab products found in correlation

Iq sybr green supermix, by Bio-Rad (1 mentions) Chip it kit, by Active Motif (1 mentions) Pgc 1α, by Novus Biologicals (1 mentions)

Close spelling variants of this product

RNA polymerase II Anti-RNA Polymerase II (sc-899; Anti-RNA polymerase II RNA polymerase II antibody Anti‐N‐terminal RNA polymerase II A‐10 Anti-RNA Polymerase II antibody RNA polymerase II (Pol II)

2 protocols using polymerase 2

ChIP-qPCR for NFAT, NFκB, and Pol II

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Chromatin immunoprecipitation was performed essentially as described [27] (link). Briefly, HeyA8 cells grown under adherent and spheroid culture conditions and adherent HeyA8 cells treated with 20µM U0126 were cross-linked in 1% formaldehyde. Chromatin was sheared to an average fragment size of 150–200 bp using both MNase digestion and sonication, and immunoprecipitation was performed using antibodies to NFAT (Bethyl), NFκB (Abcam), and Polymerase II (Santa Cruz) with appropriate isotype controls. Quantitative PCR was performed on two independent immunoprecipitations in duplicate using iQ SYBR Green Supermix (Bio-Rad). Fold changes in occupancy of NFAT, NFκB, and Pol II at the CXCR4 promoter were determined using the comparative Ct method.

Huang K., Kiefer C, & Kamal A. (2014). Novel Role for NFAT3 in ERK-Mediated Regulation of CXCR4. PLoS ONE, 9(12), e115249.

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Hypoxia-Induced TERT Promoter Analysis

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Cultured HAECs were exposed to 1% oxygen for 30 min at 37°C and processed using a ChIP-IT® kit (Active Motif #53008, Carlsbad, CA) according to the manufacturer’s instructions. Briefly, the cells were fixed in 1% formalin and homogenized in lysis buffer. Lysed cells were sheared with sonication ten times each with a pulse of 20 seconds and 30-seconds of rest on ice between shearing steps. Sheared samples were incubated with Protein G Beads and either non-immune IgG (Sigma #NI01), Polymerase II (Santa Cruz #sc56767), or PGC1α (Novus Bio #NBP-04676, Centennial, CO) antibodies on an end-to-end rotator overnight at 4°C. The beads binding target chromatin were washed on a magnetic bar with washing buffer. Elucidated chromatin targets were amplified with quantitavive PCR using a primer pair (5’-CAGAAGTTTCTCGCCCCCTT-3’ and 5’- GAGGCCAACATCTGGTCAC-3’) specific for the TERT promoter.

Kant S., Tran K.V., Kvandova M., Caliz A.D., Yoo H.J., Learnard H., Dolan A.C., Craige S.M., Hall J.D., Jiménez J.M., St. Hilaire C., Schulz E., Kröller-Schön S, & Keaney JF J.r. (2021). PGC1α Regulates the Endothelial Response to Fluid Shear Stress via Telomerase Reverse Transcriptase Control of Heme Oxygenase-1. Arteriosclerosis, thrombosis, and vascular biology, 42(1), 19-34.

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