Yeast cells were imaged with a Yokogawa spinning-disc confocal microscope equipped with Nikon PlanApo 100X/1.45NA or ApoTIRF 60X/1.49NA objective lens and a Hamamatsu cooled back-thinned CCD-camera or EM-CCD camera 46 (link). Cells were grown overnight to OD ~0.5, then placed between 2% agarose YE5S pad and glass coverslip for imaging. Images were acquired at ~20°C and processed with MetaMorph 7.7 (www.MolecularDevices.com ). Precise details of imaging conditions are provided for each figure below.
Planapo 100x 1.45na
Manufactured by Hamamatsu Photonics
The PlanApo 100X/1.45NA is an objective lens designed for high-resolution microscopy applications. It features a numerical aperture of 1.45 and a magnification of 100X, providing high-quality, detailed images. The lens is optimized for use with advanced microscopy techniques such as fluorescence imaging.
Automatically generated - may contain errors
2 protocols using planapo 100x 1.45na
1
Yeast Cell Imaging with Confocal Microscopy
2
Yeast Cell Imaging with Confocal Microscopy
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