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The UAS-CD8-RFP is a genetic construct that expresses the red fluorescent protein (RFP) under the control of the Upstream Activating Sequence (UAS) promoter. This construct can be used to visualize the expression pattern of specific genes in Drosophila (fruit fly) models.

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Lab products found in correlation

Uas cd8 gfp, by Bloomington Drosophila Stock Center (2 mentions) Uas tdp 43, by Bloomington Drosophila Stock Center (1 mentions) Uas nlsgfp, by Bloomington Drosophila Stock Center (1 mentions) Uas mcherrycaax, by Bloomington Drosophila Stock Center (1 mentions) Uas mcherry nls, by Bloomington Drosophila Stock Center (1 mentions) Htl gal4, by Bloomington Drosophila Stock Center (1 mentions) Uas lifeact gfp, by Bloomington Drosophila Stock Center (1 mentions) Engrailed en gal4, by Bloomington Drosophila Stock Center (1 mentions) W1118, by Bloomington Drosophila Stock Center (1 mentions) Uas myrrfp, by Bloomington Drosophila Stock Center (1 mentions) Ru486, by Merck Group (1 mentions)

4 protocols using uas cd8 rfp

1

Axonal Transport and TDP-43 Quantification in Drosophila Larvae

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3rd instar Drosophila larvae genotypes
OK371>Gal4/ UAS-Venus-TDP-43 WT, OK371>Gal4/ UAS-Venus-TDP-43
M337V, and OK371>Gal4/UAS-YFP-TDP-43 A315T were used for axonal transport
imaging. For TDP-43 quantification at the cell body and synaptic terminal,
UAS-CD8-RFP (Bloomington Stock Center, Indiana U, Bloomington, IN; stock #27391)
was co-expressed, resulting in the genotypes: OK371>Gal4, UAS-CD8-RFP/
UAS-TDP-43 WT or OK371>Gal4, UAS-CD8-RFP/ UAS-TDP-43 M337V,
OK371>Gal4, UAS-CD8-RFP/UAS-TDP-43 A315T, and negative control-
OK371>Gal4, UAS-CD8-RFP/+. OK371-Gal4 driver was used for all
Drosophila transgene expression except for the TBPH rescue
cross where Armadillo Gal4 was used. In TBPH viability rescue cross, transgenes
(UAS-Venus-TBPH, UAS-Venus-TDP-43, and UAS-Venus-TDP43 M337V) were expressed by
Armadillo Gal4 in TBPH Δ23 homozygote mutants. Controls in this analysis
included W1118, homozygotes and heterozygotes for the null TBPH Δ23
mutation. Third instar larvae were sorted by genotype and placed in separate
vials. The percentage of larvae that became living adults was recorded and
graphed.
Alami N.H., Smith R.B., Carrasco M.A., Williams L.A., Winborn C.S., Han S.S., Kiskinis E., Winborn B., Freibaum B.D., Kanagaraj A., Clare A.J., Badders N.M., Bilican B., Chaum E., Chandran S., Shaw C.E., Eggan K.C., Maniatis T, & Taylor J.P. (2014). Axonal transport of TDP-43 mRNA granules in neurons is impaired by ALS-causing mutations. Neuron, 81(3), 536-543.
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2

Drosophila Genetics and Imaging Protocol

Cited 3 times
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All flies were raised at 25 °C with 12 h/12 h light/dark cycle unless noted. This study: htl-LexA, pyr-Gal4/CyO, htl>FRT>stop>FRT>Gal4, UAS-Pyr:GFP, UAS-Ths:GFP and LexO-Htl:mCherry. All new transgenic injections were performed by Rainbow Transgenic Flies, Inc. Bloomington Drosophila Stock Center: UAS-CD8:GFP, UAS-CD8:RFP, UAS-mCherryCAAX, LexO-CD2:GFP, UAS-Eb1:GFP, UAS-Lifeact:GFP, UAS-nls:GFP, UAS-nls:mCherry, htl-Gal4, ths-Gal4/CyO, UAS-Dia:GFP, UAS-ΔDAD-Dia:GFP, UAS-pyrRNAi, UAS-diaRNAi, hs-Flp, {nos-Cas9}ZH-2A, and w1118. Vienna Drosophila Resource Center: htl:GFPfTRG, UAS-htlRNAi, and UAS-thsRNAi. Other sources: LexO-nsyb:GFP1–10, UAS-CD4:GFP1120 (link). LexO-mCherryCAAX15 (link). dpp-Gal4/CyO, LexO-Fz:mCherry and 1151-Gal4 from Huang et al.36 (link) (also see Supplementary Table 3).
Patel A., Wu Y., Han X., Su Y., Maugel T., Shroff H, & Roy S. (2022). Cytonemes coordinate asymmetric signaling and organization in the Drosophila muscle progenitor niche. Nature Communications, 13, 1185.
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3

Generation and Analysis of Drosophila Transgenic Lines

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Engrailed (en)-GAL4 and UAS-CD8-RFP were obtained from the Bloomington Stock Center. UAS-dispHA, UAS-dispTM4HA and UAS-GFP transgenic lines were generated using the PhiC31/attB system (Bischof et al., 2007 (link)). Transgenes were targeted to the ZH-51D landing site. Both male and female flies were assessed for phenotype. Male wings are shown in Fig. 6 and female wings in Fig. S3.
Bodeen W.J., Marada S., Truong A, & Ogden S.K. (2017). A fixation method to preserve cultured cell cytonemes facilitates mechanistic interrogation of morphogen transport. Development (Cambridge, England), 144(19), 3612-3624.
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4

Drosophila Genetics and GeneSwitch Expression

Cited 1 time
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Flies were grown and maintained at 25°C in vials containing standard cornmeal/agar medium supplemented with yeast under 12∶12 h light∶dark cycles. GeneSwitch expression was induced by transferring 1–4 day old adult males to food containing RU486 (mifepristone, Sigma, USA) in 80% ethanol to a final concentration of 200 µg/ml (or 500 µg/ml in the case of UAS-pdf rescue experiments) or with the same amount of ethanol (vehicle) in control treatments. All stocks used in this study were described previously: pdf-GeneSwitch (pdf-GS) was generated in our laboratory [30] (link), UAS-Mmp1 (chromosomes II and III) and UAS-Mmp2 (chromosomes II and III) were gently provided by A. Page-McCaw [24] (link), UAS-Mmp1RNAiB and UAS-Mmp2RNAiB by D. Bohmann [65] (link) and UAS-pdf by P. Taghert [5] (link). w1118 (#40015), UAS-CD8GFP (#5137), UAS-CD8RFP (#27398), UAS-ANFGFP (#7001) and UAS-myrRFP (#7119) were obtained from the Bloomington Stock Center. UAS-Mmp1RNAi (#101505), UAS-Mmp2RNAi (#107888), UAS-Dicer2 (#60008 and 60009), UAS-pdfRNAi (#4380), UAS-EcRRNAi (#37059) and UAS-Fas2RNAi (#36351) were obtained from the Vienna RNAi Stock Center. Experiments shown in Figure S1 G were carried out with the RNAi lines generated by the Bohmann laboratory [65] (link).
Depetris-Chauvin A., Fernández-Gamba Á., Gorostiza E.A., Herrero A., Castaño E.M, & Ceriani M.F. (2014). Mmp1 Processing of the PDF Neuropeptide Regulates Circadian Structural Plasticity of Pacemaker Neurons. PLoS Genetics, 10(10), e1004700.
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