Log In Sign up
The largest database of trusted experimental protocols

Penicillin and streptomycin

Manufactured by Biosera
Visit Supplier
Sourced in United Kingdom

Penicillin and streptomycin are antibiotics commonly used in cell culture media formulations. They provide broad-spectrum antimicrobial protection against a variety of bacterial species that may contaminate cell cultures.

Automatically generated - may contain errors

Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Rpmi 1640 medium, by Biosera (2 mentions) Hela cell, by American Type Culture Collection (1 mentions) Cd14 beads, by Miltenyi Biotec (1 mentions) Dmem medium, by Biosera (1 mentions) Fetal calf serum (fcs), by Biosera (1 mentions) Penicillin streptomycin, by Biosera (1 mentions) Ficoll paque plus, by GE Healthcare (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions) Trypsin, by Merck Group (1 mentions) Agarose, by Thermo Fisher Scientific (1 mentions) Gentamicin, by Thermo Fisher Scientific (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) High glucose dmem, by Thermo Fisher Scientific (1 mentions) Tris base, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Ce no3 3 6h2o, by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

Penicillin/streptomycin (103 citations) Penicillin (79 citations) Streptomycin (76 citations)

5 protocols using penicillin and streptomycin

1

Culturing and Stimulating Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human embryonic kidney (HEK) 293T cells (Unitech Ltd) and HeLa cells (ATCC) were cultured in DMEM medium (Biosera) supplemented with 10% FCS (Biosera), penicillin and streptomycin (100 µg/ml) (Biosera). Human PBMCs were isolated from whole blood by density gradient centrifugation using Ficoll-Paque Plus (GE Healthcare). Human monocytes were subsequently extracted from PBMCs by positive selection using CD14 beads (Miltenyi Biotec) and cultured in RPMI-1640 medium (Biosera) supplemented with 10% FCS and penicillin–streptomycin (100 µg/ml). Following stimulation, supernatants were collected and RANTES levels determined by ELISA (R&D Systems) according to the manufacturer's recommendations.
Wynne C., Lazzari E., Smith S., McCarthy E.M., Ní Gabhann J., Kallal L.E., Higgs R., Cryan S.A., Biron C.A, & Jefferies C.A. (2014). TRIM68 Negatively Regulates IFN-β Production by Degrading TRK Fused Gene, a Novel Driver of IFN-β Downstream of Anti-Viral Detection Systems. PLoS ONE, 9(7), e101503.
+ Open protocol
+ Expand
2

Isolation and Characterization of Human Spermatogonial Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Testicular biopsies obtained from azoospermic patients by testicular sperm extraction (TESE). A small portion of the testicular tissue placed in Hank’s balanced salt solution (HBSS) supplemented with penicillin and streptomycin (Biosera, UK) and minced in small pieces. In order to isolation of spermatogonial stem cells from testis, the tissue was digested with 0.25%trypsin (Sigma Aldrich, USA) for 5 minutesat 37°C. The obtained suspension centrifuged at 1500 rpm for 5 minutes and the supernatant discarded and cell pellet cultured in DMEM/F12 (Gibco, USA) supplemented with 20% FBS (Gibco, USA) and 1% penicillin/streptomycin. After 15 days, human spermatogonial stem cell clusters collected and mechanically isolated and cultured in new cell culture flask. Subsequently, the cells subcultured after confluence phase and in passage one the expression of MSC- related cell surface antigens analyzed by flow cytometry.
Maleki M., Ghanbarvand F., Reza Behvarz M., Ejtemaei M, & Ghadirkhomi E. (2014). Comparison of Mesenchymal Stem Cell Markers in Multiple Human Adult Stem Cells. International Journal of Stem Cells, 7(2), 118-126.
+ Open protocol
+ Expand
3

Cytotoxicity Assessment of 5-FU Using MTT Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ce (NO3)3.6H2 O (Sigma-Aldrich, USA), C35H49O29 (Sigma-Aldrich,
USA), NaOH (Sigma-Aldrich, USA), EDTA (Merck, Germany), Agarose (Fermentas, UK), Tris base
(Merck, Germany), Green viewer (SinaClon, Iran), Loading dye buffer (6X; SinaClon, Iran),
Fetal Bovine Serum (Gibco, Canada), DMEM-high Glucose (Gibco, Canada), Gentamicin (Gibco,
Canada), Triton X-100 (Merck, Germany), 5-FU (Cayman Thermo Fisher Scientific, USA), MTT
powder (Auto Thermo Fisher Scientific, USA), PI (MabTag GmbH, Germany), Annexin (MabTag
GmbH, Germany), Penicillin and Streptomycin (Biosera, USA), DMSO (Merck, Germany), Trypsin
(Gibco, Canada).
This research was approved by University of Zabol with
Ethics committee letter number MAA-131.
Keramati Z., Motalleb G., Rahdar A, & Kerachian M.A. (2023). Anticancer Effect of Fluorouracil and Gum-Based Cerium Oxide Nanoparticles on Human Malignant Colon Carcinoma Cell Line (Caco2). Cell Journal (Yakhteh), 25(3), 194-202.
+ Open protocol
+ Expand
4

Establishment and Maintenance of MCF-7/ADR Cell Line

Check if the same lab product or an alternative is used in the 5 most similar protocols
MCF-7/S cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and the multidrug-resistant variant MCF-7/ADR was established and maintained in our laboratory. All cells were cultured in RPMI-1640 medium (Biosera, UK) supplemented with 10% fetal bovine serum (Gibco), and 1% penicillin and streptomycin (Biosera). MCF-7/ADR cells were grown in the RPMI 1640 complete medium with 1 μg/mL ADR to maintain drug-resistance. The cells were cultured at 37°C in a humidified atmosphere containing 5% CO2.
Bai M., Shen M., Teng Y., Sun Y., Li F., Zhang X., Xu Y., Duan Y, & Du L. (2015). Enhanced therapeutic effect of Adriamycin on multidrug resistant breast cancer by the ABCG2-siRNA loaded polymeric nanoparticles assisted with ultrasound. Oncotarget, 6(41), 43779-43790.
+ Open protocol
+ Expand
5

Culturing MCF-7 Breast Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
MCF-7/S cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, People’s Republic of China) and its multidrug-resistant variant MCF-7/ADR was established and maintained in our laboratory. All cells were cultured in RPMI-1640 (Roswell Park Memorial Institute-1640) medium (Biosera, East Sussex, UK) supplemented with 10% fetal bovine serum (Biosera), and 1% penicillin and streptomycin (Biosera). MCF-7/ADR cells were grown in the RPMI-1640 complete medium with 1 μg/mL Adriamycin (ADR) so as to maintain its drug resistance. Cell cultures were maintained at 37°C in a humidified atmosphere containing 5% CO2.
Teng Y., Bai M., Sun Y., Wang Q., Li F., Xing J., Du L., Gong T, & Duan Y. (2015). Enhanced delivery of PEAL nanoparticles with ultrasound targeted microbubble destruction mediated siRNA transfection in human MCF-7/S and MCF-7/ADR cells in vitro. International Journal of Nanomedicine, 10, 5447-5457.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!