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Anti bcl2 bcl 10c4

Manufactured by BioLegend

Anti-BCL2 (BCL/10C4) is a monoclonal antibody that binds to the BCL2 protein. BCL2 is a member of the BCL2 family of proteins and plays a role in regulating apoptosis or programmed cell death. The Anti-BCL2 (BCL/10C4) antibody can be used in various applications to detect and study the BCL2 protein.

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2 protocols using anti bcl2 bcl 10c4

1

Immunophenotyping Murine Lymphocytes

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The following antibodies were used: from eBioscience: Anti-BrdU (clone Bu20A), anti-CD127 (A7R34), anti-CD4 (RM4–5), anti-CD8α (53–6.7), anti-EGR2 (erongr2), anti-FOXP3 (FKJ-16s), anti-TCR Vα2 (B20.1), anti-TCRβ (H57-597), anti-Vβ6 TCR (RR4-7), anti-Vβ8.1/Vβ8.2 TCR (Kj16-1333); from BD Biosciences: anti-active caspase 3 (C92-105), anti-CD4 (Gk1.5), anti-CD24 (M1/69), anti-CD16/CD32 (2.4G2), anti-CD44 (IM7), anti-CD45.1 (A20), anti-CD45.2 (104), anti-CD45R/B220 (RA3-6B2), anti-CD5 (53-7,3), anti-CD62L (MEL-14), anti-CD69 (H1.2F3), anti-RORγt (Q31-378); from Biolegend: anti-BCL2 (BCL/10C4).
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2

Multiparameter flow cytometry analysis

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The following monoclonal antibodies were used for multiparameter flow cytometry: anti-CD4 (RM4-5, Biolegend), anti-CD25 (PC61.5, eBioscience), anti-CCR6 (29-2L17, Biolegend), anti-CD103 (2E7, Biolegend), anti-Ly5.1 (A20, Biolegend), anti-Ly5.2 (104, Biolegend), anti-CTLA4 (UC10-4B9, eBioscience), anti-Ki67 (solA15, eBioscience), anti-Bcl-2 (BCL10C4, Biolegend), anti-ICOS (7E.17G, eBioscience), anti-GITR (DTA-1, eBioscience), anti-Helios (22F6, Biolegend), anti-Neuropilin (FAB566A, R&D systems), anti-PD1 (J43, eBioscience), anti-CXCR5 (2G8, BD Biosciences). The Foxp3 staining kit was used for staining for CTLA-4, Ki67, Helios, Bcl-2 and Bcl6. Stained cells were analyzed on FACS Canto (Becton Dickinson) and were sorted on FACS Aria (Becton Dickinson). Data were analyzed with Flowjo software.
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