Mouse anti lamp1 h4a3

Recombinant human sIL-6R and goat anti-IL-6 were purchased from R&D Systems. The mouse anti-STAT3, rabbit anti-phosphor-STAT3 (Tyr705) (pSTAT3), and horseradish peroxidase (HRP)-linked anti-rabbit and anti-mouse antibodies were from Cell Signaling Technology, and recombinant human IL-6 and mouse anti-IL-6R (B-R6) were from Millipore. Rabbit anti-IL-6R (C-20) was obtained from Santa Cruz Biotechnology, mouse anti-cd49b was from BD Biosciences, mouse anti-LAMP-1 (H4A3) was from the Developmental Studies Hybridoma Bank, and NT was from Sigma. The murine monoclonal anti-SorLA (31 (link)) and the rabbit polyclonal anti-SorLA have previously been described (32 (link)). Secondary antibodies conjugated with Alexa Fluor dyes were all from Invitrogen.

Rabbit anti-LAMTOR4 (cat. 13140) (1:200) was from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-α-tubulin (DM1A; cat. T6199) (1:1,000) and D-biotin (cat. 47868) were from Sigma-Aldrich (St. Louis, MO, USA). Rabbit anti-TfR (cat. ab84036) (1:200) and rabbit anti-PMP70 (cat. ab3421) (1:500) were from Abcam (Cambridge, UK). Rabbit anti-Sec31A (cat. 17913-1-AP) (1:500) was from Proteintech (Rosemont, IL, USA). Mouse anti-calnexin (clone C8.B6; cat. MAB3126) (1:1,000) was from EMD Millipore (Burlington, MA, USA). Mouse anti-pan-neurofascin (external) (clone A12/18; cat. 75–172) (1:50) was from the University of California, Davis/NIH NeuroMab Facility (Davis, CA, USA). Mix-n-Stain-CF640R (cat. 92245) was from Biotium (Fremont, CA, USA) and was used to label the antibody against neurofascin. Mouse anti-LAMP1 (H4A3) (1:500) was from the Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA, USA). Mouse anti-EEA1 (cat. 610457) (1:1,000) was from BD Biosciences (San Jose, CA, USA). All secondary antibodies (donkey anti-rabbit and anti-mouse conjugated with Alexa Fluor 488, 555, or 647) (1:1,000) were from Invitrogen (Carlsbad, CA, USA). NeutrAvidin Protein (cat. 31050), LysoTracker Blue DND-22 (cat. L7525), AF647-dextran (cat. D22914), MitoTracker Red CMXRos (cat. M7512), and DQ Red BSA (cat. D12051) were from Thermo Fisher Scientific (Waltham, MA, USA).